##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51717
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.61763056635149	32.0	30.0	32.0	18.0	33.0
2	30.725312759827524	32.0	32.0	33.0	29.0	33.0
3	31.21491965891293	32.0	32.0	33.0	29.0	33.0
4	31.048862076299862	32.0	32.0	33.0	29.0	33.0
5	31.128874451340952	32.0	32.0	33.0	29.0	33.0
6	34.37799949726396	37.0	33.0	37.0	31.0	37.0
7	34.44563683121604	37.0	33.0	37.0	31.0	37.0
8	34.368022120385945	37.0	33.0	37.0	31.0	37.0
9	34.532764854883304	37.0	33.0	37.0	32.0	37.0
10	34.591875012085	37.0	33.0	37.0	32.0	37.0
11	34.41079335614982	37.0	33.0	37.0	32.0	37.0
12	34.439662006690256	37.0	33.0	37.0	32.0	37.0
13	36.11648007425025	38.0	37.0	38.0	33.0	38.0
14	36.056751164994104	38.0	37.0	38.0	33.0	38.0
15	36.1270181951776	38.0	37.0	38.0	33.0	38.0
16	36.06549103776321	38.0	37.0	38.0	33.0	38.0
17	35.87818318928012	38.0	37.0	38.0	33.0	38.0
18	36.03925208345418	38.0	37.0	38.0	33.0	38.0
19	36.091149912021194	38.0	37.0	38.0	33.0	38.0
20	36.09256144014541	38.0	37.0	38.0	33.0	38.0
21	36.12177813871648	38.0	37.0	38.0	33.0	38.0
22	36.14198426049462	38.0	37.0	38.0	33.0	38.0
23	36.51232670108475	38.0	37.0	39.0	33.0	39.0
24	36.311560995417366	38.0	37.0	39.0	33.0	39.0
25	36.087534079703	38.0	37.0	39.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	7.0
23	32.0
24	76.0
25	138.0
26	259.0
27	464.0
28	691.0
29	1056.0
30	1587.0
31	2295.0
32	3212.0
33	4746.0
34	7731.0
35	13025.0
36	15748.0
37	647.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.106734729392656	18.877738461240984	14.681826092000696	26.333700717365666
2	16.45687104820465	22.261538759015412	36.270471991801536	25.011118200978398
3	19.836804145638766	24.54512055997061	29.056209756946462	26.561865537444167
4	15.107218129435196	18.902875263453023	33.638842160218104	32.35106444689367
5	15.20196453777288	36.022971170021464	32.757120482626604	16.017943809579055
6	33.70071736566313	34.74486145754781	17.081423903165305	14.472997273623761
7	28.717829727169015	30.405862675715916	21.990834735193456	18.885472861921613
8	26.61020554169809	34.77193185993001	19.72465533576967	18.89320726260224
9	26.436181526383972	16.497476651777944	18.877738461240984	38.18860336059709
10	15.77817738847961	27.718158439197943	32.76098768296691	23.74267648935553
11	36.22599918788793	21.9753659338322	22.86868921244465	18.92994566583522
12	24.442639750952296	23.301815650559774	29.22636657192026	23.029178026567667
13	28.816443335847016	20.82294023241874	25.167739814761102	25.19287661697314
14	23.694336485101612	20.126844171162286	24.70174217375331	31.477077169982792
15	24.955043796043856	28.01786646557225	21.718197111201345	25.30889262718255
16	25.25861902275847	26.130672699499197	23.87609490109635	24.734613376645978
17	24.390432546358063	25.975984685886655	24.95697739621401	24.67660537154127
18	24.933774194172127	25.867703076357873	25.299224626331767	23.899298103138232
19	25.465514240965252	25.5215886458998	25.111665409826557	23.90123170330839
20	25.1948102171433	26.059129493203397	24.49484695554653	24.251213334106772
21	25.11553261016687	25.32822862888412	24.93764139451244	24.618597366436568
22	24.55092136048108	25.496451843687762	24.93764139451244	25.014985401318718
23	24.641800568478452	24.933774194172127	25.26828702360926	25.156138213740164
24	24.81002378328209	26.260223910899704	24.748148577837075	24.181603727981127
25	25.33402942939459	25.131001411528125	24.860297387706172	24.674671771371116
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	4.5
7	8.0
8	8.0
9	8.0
10	30.0
11	52.0
12	52.0
13	52.0
14	79.5
15	107.0
16	107.0
17	107.0
18	208.0
19	309.0
20	309.0
21	309.0
22	512.0
23	715.0
24	715.0
25	715.0
26	1014.0
27	1313.0
28	1313.0
29	1313.0
30	1756.5
31	2200.0
32	2200.0
33	2200.0
34	2666.5
35	3133.0
36	3133.0
37	3133.0
38	3996.0
39	4859.0
40	4859.0
41	4859.0
42	5501.0
43	6143.0
44	6143.0
45	6143.0
46	6573.5
47	7004.0
48	7004.0
49	7004.0
50	7245.0
51	7486.0
52	7486.0
53	7486.0
54	7182.5
55	6879.0
56	6879.0
57	6879.0
58	6053.0
59	5227.0
60	5227.0
61	5227.0
62	4274.0
63	3321.0
64	3321.0
65	3321.0
66	2531.0
67	1741.0
68	1741.0
69	1741.0
70	1283.0
71	825.0
72	825.0
73	825.0
74	562.5
75	300.0
76	300.0
77	300.0
78	186.0
79	72.0
80	72.0
81	72.0
82	45.5
83	19.0
84	19.0
85	19.0
86	10.5
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	51717.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.17545487944003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09695770467475	67.30668832298858
2	8.323027454860252	13.013129145155364
3	2.6589166460549096	6.235860548755729
4	1.2490724709374228	3.905872343716766
5	0.635666584219639	2.484676218651507
6	0.35122433836260203	1.647427344973606
7	0.19292604501607716	1.055745692905621
8	0.126143952510512	0.7889088694239805
9	0.08409596834034133	0.5916816520679854
>10	0.2819688350234974	2.970009861360868
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.001933600170156815	0.0
13	0.0	0.0	0.0	0.001933600170156815	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE