##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920820_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58783
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19837367946515	33.0	32.0	33.0	32.0	34.0
2	32.59920385145365	33.0	32.0	34.0	32.0	34.0
3	32.83877991936444	33.0	32.0	34.0	32.0	34.0
4	32.955939642413625	33.0	33.0	34.0	32.0	34.0
5	33.03997754452818	33.0	33.0	34.0	32.0	34.0
6	35.764829967847845	37.0	35.0	37.0	33.0	37.0
7	35.824643179150435	37.0	37.0	37.0	33.0	37.0
8	35.6562271404998	37.0	35.0	37.0	33.0	37.0
9	35.76683735093479	37.0	35.0	37.0	33.0	37.0
10	35.80431417246483	37.0	35.0	37.0	33.0	37.0
11	35.70879335862409	37.0	34.0	37.0	33.0	37.0
12	35.741081605226	37.0	34.0	37.0	33.0	37.0
13	37.48229930422061	38.0	38.0	38.0	37.0	38.0
14	37.44887127230662	38.0	38.0	38.0	37.0	38.0
15	37.44604732660803	38.0	38.0	38.0	37.0	38.0
16	37.48439174591294	38.0	38.0	38.0	37.0	38.0
17	37.46634230985149	38.0	38.0	38.0	37.0	38.0
18	37.4862460235102	38.0	38.0	38.0	37.0	38.0
19	37.4597927972373	38.0	38.0	38.0	37.0	38.0
20	37.48024088597043	38.0	38.0	38.0	37.0	38.0
21	37.503240732864946	38.0	38.0	38.0	37.0	38.0
22	37.488933875440175	38.0	38.0	38.0	37.0	38.0
23	38.13013966623003	39.0	38.0	39.0	37.0	39.0
24	38.04562543592535	39.0	38.0	39.0	37.0	39.0
25	38.00139496112822	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	0.0
19	4.0
20	8.0
21	16.0
22	32.0
23	54.0
24	64.0
25	78.0
26	95.0
27	132.0
28	164.0
29	240.0
30	311.0
31	402.0
32	621.0
33	1054.0
34	2244.0
35	7735.0
36	39944.0
37	5582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76916795672218	17.69218991885409	12.518925539696852	27.019716584726876
2	18.352244696595953	19.003793613799907	36.702788221084326	25.941173468519814
3	19.930932412432163	22.836534372182435	28.450402327203445	28.782130888181957
4	14.027865199122195	15.885545140601876	35.19895207798173	34.887637582294204
5	14.866543048160183	34.84851062381981	32.841127536872904	17.443818791147102
6	33.33446744807172	34.13742068285048	17.823180171137913	14.70493169793988
7	28.261572223261826	29.710970858921797	22.518415188064576	19.5090417297518
8	26.769644284912303	33.41782488134324	20.01428984570369	19.79824098804076
9	25.640066005477774	15.919568582753518	19.72679175952231	38.713573652246396
10	16.373781535477942	26.63525168841332	32.65399860503887	24.336968171069866
11	35.24828606910161	20.919313406937377	24.183862681387474	19.648537842573532
12	24.449245530170288	23.095112532534916	29.17340047292585	23.28224146436895
13	28.6919687664801	20.347719578789786	25.383189017232873	25.577122637497236
14	23.756868482384363	19.76761989010428	24.632972117789155	31.8425395097222
15	24.578534610346527	27.346341629382643	22.309171018831975	25.765952741438852
16	24.944286613476685	25.805079699913243	24.600649847745096	24.649983838864976
17	24.74014596056683	24.990218260381404	25.352567919296394	24.91706785975537
18	24.718030723168262	25.488661687902965	25.529489818484937	24.263817770443836
19	25.14842726638654	24.85072214755967	25.359372607726723	24.641477978327067
20	25.070173349437763	25.31344096082201	24.93918309715394	24.67720259258629
21	24.583638126669275	25.05826514468469	25.088886242621168	25.269210486024875
22	24.420325604341393	25.13822023374105	25.50056989265604	24.94088426926152
23	24.942585441369104	24.549614684517632	25.197761257506425	25.310038616606846
24	24.308048245240972	25.633261317047445	25.54820271166834	24.51048772604324
25	24.697616657877276	24.75545650953507	25.774458601976765	24.77246823061089
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	8.0
7	14.0
8	14.0
9	14.0
10	21.0
11	28.0
12	28.0
13	28.0
14	73.5
15	119.0
16	119.0
17	119.0
18	214.0
19	309.0
20	309.0
21	309.0
22	510.5
23	712.0
24	712.0
25	712.0
26	1068.0
27	1424.0
28	1424.0
29	1424.0
30	1909.0
31	2394.0
32	2394.0
33	2394.0
34	2927.5
35	3461.0
36	3461.0
37	3461.0
38	4302.5
39	5144.0
40	5144.0
41	5144.0
42	5935.5
43	6727.0
44	6727.0
45	6727.0
46	7282.5
47	7838.0
48	7838.0
49	7838.0
50	8088.5
51	8339.0
52	8339.0
53	8339.0
54	8166.0
55	7993.0
56	7993.0
57	7993.0
58	7108.5
59	6224.0
60	6224.0
61	6224.0
62	5225.0
63	4226.0
64	4226.0
65	4226.0
66	3279.0
67	2332.0
68	2332.0
69	2332.0
70	1674.0
71	1016.0
72	1016.0
73	1016.0
74	680.0
75	344.0
76	344.0
77	344.0
78	228.5
79	113.0
80	113.0
81	113.0
82	67.5
83	22.0
84	22.0
85	22.0
86	12.0
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	58783.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.92019801643333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06466737438076	66.20111256655836
2	8.072363765038924	12.418556385349506
3	2.6826786978060864	6.190565299491349
4	1.3512915782024062	4.157664630930711
5	0.6546355272469921	2.5177347192215436
6	0.32952937013446565	1.5208478641784189
7	0.22779547062986555	1.2265450895667114
8	0.15702406227883933	0.9662657571066465
9	0.10394550601556972	0.7195958015072385
>10	0.3538570417551309	3.975639215419424
>50	0.0022116065109695684	0.1054726706700917
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGATAAAGAGGGCATCCCCCCTGAC	62	0.1054726706700917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE