FastQCFastQC Report
Mon 15 Aug 2016
SRR2920819_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920819_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53650
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC800.14911463187325258No Hit
CCCTCTCTGATTACAACATCCAGAA700.130475302889096No Hit
CTGTAGGACCTGGAATATGGCGAGA700.130475302889096No Hit
CTCTCAGGCGAAGGACCAGGTGCAG680.1267474370922647No Hit
AGTCCACCCTGCACCTGGTCCTTCG640.11929170549860205No Hit
GTCCTTCAGTGTGCATTTCTCATTT610.1136999068033551No Hit
CTGAAGGACCTGGAATATGGCGAGA590.10997204100652376No Hit
GTGTATATCAATGAGTTACAATGAA540.10065237651444547No Hit
GTCCTACAGTGGACATTTCTAAATT540.10065237651444547No Hit
GATATACACTGTTCTACAATGCCGG540.10065237651444547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTGA350.002133698416.28571516
GAGCCCA350.002133698416.28571512
AGTGACA350.002133698416.28571518
GTGACAC350.002133698416.28571519
CAGTGAC350.002133698416.28571517
AGCCCAG400.00519172714.2513
TGGCGAG750.00257705610.13333318
ATGGCGA800.00439670789.517
ATATGGC1150.0098781987.434782515
AATATGG1150.0098781987.434782514
Produced by FastQC (version 0.11.5)