##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920819_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53650
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.022479030754894	33.0	32.0	34.0	32.0	34.0
2	32.292264678471575	33.0	32.0	34.0	32.0	34.0
3	32.41726001863933	33.0	32.0	34.0	32.0	34.0
4	32.49502329916123	33.0	32.0	34.0	32.0	34.0
5	32.448331780055916	33.0	32.0	34.0	32.0	34.0
6	35.49986952469711	37.0	35.0	37.0	33.0	37.0
7	35.3830568499534	37.0	35.0	37.0	33.0	37.0
8	35.42203168685927	37.0	35.0	37.0	33.0	37.0
9	35.48477166821994	37.0	35.0	37.0	33.0	37.0
10	35.46309412861137	37.0	35.0	37.0	33.0	37.0
11	35.49079217148183	37.0	34.0	37.0	33.0	37.0
12	35.50389561975769	37.0	34.0	37.0	33.0	37.0
13	37.11856477166822	38.0	38.0	38.0	37.0	38.0
14	37.1003541472507	38.0	38.0	38.0	37.0	38.0
15	37.07013979496738	38.0	38.0	38.0	37.0	38.0
16	37.08918918918919	38.0	38.0	38.0	37.0	38.0
17	37.11183597390494	38.0	38.0	38.0	37.0	38.0
18	37.111090400745574	38.0	38.0	38.0	37.0	38.0
19	37.095153774464116	38.0	38.0	38.0	37.0	38.0
20	37.13053122087605	38.0	38.0	38.0	37.0	38.0
21	37.162572227399814	38.0	38.0	38.0	37.0	38.0
22	37.160130475302886	38.0	38.0	38.0	37.0	38.0
23	37.77330848089469	39.0	38.0	39.0	37.0	39.0
24	37.69884436160298	39.0	38.0	39.0	37.0	39.0
25	37.59260018639329	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	7.0
19	5.0
20	20.0
21	45.0
22	72.0
23	102.0
24	140.0
25	206.0
26	255.0
27	323.0
28	396.0
29	462.0
30	587.0
31	787.0
32	1022.0
33	1336.0
34	2407.0
35	6794.0
36	33163.0
37	5517.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.845293569431504	18.643056849953403	12.315004659832246	27.196644920782852
2	16.710158434296364	20.58713886300093	37.86020503261883	24.842497670083876
3	18.808946877912394	23.867660764212488	30.993476234855542	26.32991612301957
4	12.501397949673812	17.90121155638397	37.81174277726002	31.7856477166822
5	12.49394221808015	34.723205964585276	34.003727865796826	18.779123951537745
6	30.20130475302889	35.90680335507922	19.65703634669152	14.234855545200373
7	26.06523765144455	31.351351351351354	23.45573159366263	19.12767940354147
8	25.14631873252563	34.88350419384902	21.340167753960856	18.63000931966449
9	25.407269338303824	17.526561043802424	21.041938490214353	36.0242311276794
10	15.798695246971109	27.699906803355077	34.28331780055918	22.218080149114634
11	33.392357875116495	20.971109040074555	26.704566635601118	18.93196644920783
12	23.780055917986953	23.809878844361602	31.643988816402608	20.766076421248837
13	27.041938490214353	22.512581547064308	26.907735321528424	23.53774464119292
14	22.76794035414725	21.517241379310345	25.994408201304754	29.72041006523765
15	23.010251630941287	29.72972972972973	23.45573159366263	23.804287045666356
16	22.76794035414725	27.157502329916124	27.602982292637464	22.471575023299163
17	23.373718546132338	26.19198508853681	26.838769804287043	23.595526561043805
18	22.87604846225536	27.1071761416589	28.195712954333647	21.821062441752098
19	24.434296365330848	25.58061509785648	26.76421248835042	23.220876048462255
20	23.750232991612304	27.159366262814537	27.0121155638397	22.078285181733456
21	23.586206896551722	26.10810810810811	26.3336439888164	23.972041006523764
22	22.64864864864865	27.504193849021437	27.230195712954337	22.616961789375583
23	23.176141658900278	26.063373718546135	27.131407269338304	23.629077353215283
24	22.844361602982293	27.591798695246972	27.63466915191053	21.929170549860206
25	24.08574091332712	26.186393289841565	27.136999068033553	22.590866728797764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	8.0
7	13.0
8	13.0
9	13.0
10	34.5
11	56.0
12	56.0
13	56.0
14	114.0
15	172.0
16	172.0
17	172.0
18	306.5
19	441.0
20	441.0
21	441.0
22	787.0
23	1133.0
24	1133.0
25	1133.0
26	1690.0
27	2247.0
28	2247.0
29	2247.0
30	2985.0
31	3723.0
32	3723.0
33	3723.0
34	4236.5
35	4750.0
36	4750.0
37	4750.0
38	5318.5
39	5887.0
40	5887.0
41	5887.0
42	6302.5
43	6718.0
44	6718.0
45	6718.0
46	6774.0
47	6830.0
48	6830.0
49	6830.0
50	6597.5
51	6365.0
52	6365.0
53	6365.0
54	5972.0
55	5579.0
56	5579.0
57	5579.0
58	4889.5
59	4200.0
60	4200.0
61	4200.0
62	3613.5
63	3027.0
64	3027.0
65	3027.0
66	2344.0
67	1661.0
68	1661.0
69	1661.0
70	1117.5
71	574.0
72	574.0
73	574.0
74	384.0
75	194.0
76	194.0
77	194.0
78	128.5
79	63.0
80	63.0
81	63.0
82	36.0
83	9.0
84	9.0
85	9.0
86	7.0
87	5.0
88	5.0
89	5.0
90	2.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	53650.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.31686859273066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.52448363889533	72.70643056849954
2	5.755395683453238	9.245107176141659
3	1.6523555349268975	3.9813606710158433
4	0.7426317010907404	2.3858341099720413
5	0.36667440241355304	1.472506989748369
6	0.22511023439313066	1.0848089468779123
7	0.15084706428405661	0.848089468779124
8	0.09050823857043397	0.5815470643056849
9	0.09282896263634255	0.6710158434296365
>10	0.37595729867718725	5.841565703634669
>50	0.023207240659085637	1.1817334575955265
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	80	0.14911463187325258	No Hit
CCCTCTCTGATTACAACATCCAGAA	70	0.130475302889096	No Hit
CTGTAGGACCTGGAATATGGCGAGA	70	0.130475302889096	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	68	0.1267474370922647	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	64	0.11929170549860205	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	61	0.1136999068033551	No Hit
CTGAAGGACCTGGAATATGGCGAGA	59	0.10997204100652376	No Hit
GTGTATATCAATGAGTTACAATGAA	54	0.10065237651444547	No Hit
GTCCTACAGTGGACATTTCTAAATT	54	0.10065237651444547	No Hit
GATATACACTGTTCTACAATGCCGG	54	0.10065237651444547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGTGA	35	0.0021336984	16.285715	16
GAGCCCA	35	0.0021336984	16.285715	12
AGTGACA	35	0.0021336984	16.285715	18
GTGACAC	35	0.0021336984	16.285715	19
CAGTGAC	35	0.0021336984	16.285715	17
AGCCCAG	40	0.005191727	14.25	13
TGGCGAG	75	0.002577056	10.133333	18
ATGGCGA	80	0.0043967078	9.5	17
ATATGGC	115	0.009878198	7.4347825	15
AATATGG	115	0.009878198	7.4347825	14
>>END_MODULE