FastQCFastQC Report
Mon 15 Aug 2016
SRR2920819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53650
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGGACCTGGAATATGGCGAGA710.13233923578751167No Hit
GTCCTTCAGTGTGCATTTCTCATTT690.12861136999068032No Hit
GGACAAGGAGGGCATCCCCCCTGAC660.12301957129543337No Hit
GATATACACTGTTCTACAAATCCCG650.12115563839701769No Hit
CCCTAGAGGTGGAGCCCAGTGACAC640.11929170549860205No Hit
CTGTAGGACCTGGAATATGGCGAGA640.11929170549860205No Hit
CCCTCTCTGATTACAACATCCAGAA620.11556383970177074No Hit
CTCTCAGGCGAAGGACCAGGTGCAG580.10810810810810811No Hit
AGTCCACCCTGCACCTGGTCCTTCG550.10251630941286113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGACA307.5907505E-419.018
GCCCAGT453.4373013E-516.8888914
GAGGTGG350.002133698416.2857156
GTGACAC350.002133698416.28571519
GAGCCCA456.613065E-414.77777912
GGTGGAG400.00519172714.258
CAGTGAC400.00519172714.2517
GGAGCCC400.00519172714.2511
AGCCCAG500.001466909813.29999913
TATGGCG855.1042905E-511.1764716
CTGGAAT600.00575134511.08333410
TGGCGAG909.127852E-510.55555618
ATGGCGA951.5777622E-410.017
ATATGGC1201.6810358E-48.70833415
AATATGG1303.9927382E-48.03846214
Produced by FastQC (version 0.11.5)