##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53650
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.964827586206894	33.0	32.0	34.0	32.0	34.0
2	33.03616029822926	33.0	33.0	34.0	32.0	34.0
3	33.269170549860206	34.0	33.0	34.0	32.0	35.0
4	33.32775396085741	34.0	33.0	34.0	32.0	35.0
5	33.21444547996272	34.0	33.0	34.0	32.0	35.0
6	36.03287977632805	37.0	37.0	37.0	33.0	37.0
7	36.00626281453868	37.0	37.0	37.0	33.0	37.0
8	35.923355079217146	37.0	37.0	37.0	33.0	37.0
9	35.92244175209692	37.0	37.0	37.0	33.0	37.0
10	35.90948741845293	37.0	37.0	37.0	33.0	37.0
11	35.85537744641193	37.0	35.0	37.0	33.0	37.0
12	35.830158434296365	37.0	35.0	37.0	33.0	37.0
13	37.462758620689655	38.0	38.0	38.0	37.0	38.0
14	37.485181733457594	38.0	38.0	38.0	37.0	38.0
15	37.47183597390494	38.0	38.0	38.0	37.0	38.0
16	37.45696178937558	38.0	38.0	38.0	37.0	38.0
17	37.39342031686859	38.0	38.0	38.0	37.0	38.0
18	37.433830382106244	38.0	38.0	38.0	37.0	38.0
19	37.418900279589934	38.0	38.0	38.0	37.0	38.0
20	37.41558247903075	38.0	38.0	38.0	37.0	38.0
21	37.411332712022364	38.0	38.0	38.0	37.0	38.0
22	37.431761416589005	38.0	38.0	38.0	37.0	38.0
23	38.121640260950606	39.0	38.0	39.0	37.0	39.0
24	38.128667287977635	39.0	38.0	39.0	37.0	39.0
25	37.985219012115564	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	2.0
23	3.0
24	8.0
25	22.0
26	41.0
27	90.0
28	149.0
29	259.0
30	370.0
31	572.0
32	758.0
33	1142.0
34	1961.0
35	5134.0
36	32224.0
37	10913.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.34855545200373	18.93942218080149	12.421248835041938	26.290773532152844
2	17.269338303821062	20.479030754892825	37.847157502329914	24.4044734389562
3	18.736253494874184	23.945945945945944	31.017707362534946	26.30009319664492
4	12.684063373718546	18.262814538676608	36.98229263746505	32.07082945013979
5	12.093196644920782	35.15750232991613	33.444547996272135	19.304753028890957
6	30.14538676607642	36.19198508853681	19.399813606710158	14.262814538676608
7	26.44547996272134	31.06430568499534	23.15377446411929	19.336439888164026
8	25.58807082945014	35.338303821062446	20.881640260950608	18.191985088536814
9	25.301025163094128	17.46877912395154	21.364398881640263	35.86579683131407
10	15.616029822926375	27.841565703634668	34.2143522833178	22.328052190121156
11	33.222739981360675	20.687791239515377	26.823858341099722	19.26561043802423
12	23.589934762348555	24.1547064305685	31.903075489282383	20.35228331780056
13	27.161230195712953	22.14165890027959	27.088536812674747	23.608574091332713
14	22.75489282385834	21.80242311276794	25.796831314072694	29.645852749301028
15	22.868592730661696	29.81174277726002	23.170549860205032	24.149114631873253
16	22.85927306616962	27.526561043802424	26.93010251630941	22.684063373718548
17	23.54333643988816	26.18825722273998	26.874184529356942	23.394221808014912
18	22.436160298229264	27.483690587138867	28.11183597390494	21.968313140726934
19	24.713886300093197	25.47064305684995	26.821994408201306	22.993476234855546
20	24.059645852749302	27.21901211556384	26.225535880708296	22.495806150978563
21	22.93942218080149	26.749301025163092	26.521901211556383	23.789375582479032
22	22.40633737185461	27.623485554520038	27.448275862068964	22.521901211556383
23	23.056849953401677	26.3336439888164	26.939422180801493	23.67008387698043
24	22.417520969245107	27.260018639328987	27.712954333643992	22.60950605778192
25	23.274930102516308	26.320596458527497	27.53774464119292	22.86672879776328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	1.0
5	1.0
6	9.5
7	18.0
8	18.0
9	18.0
10	61.5
11	105.0
12	105.0
13	105.0
14	149.0
15	193.0
16	193.0
17	193.0
18	322.0
19	451.0
20	451.0
21	451.0
22	785.5
23	1120.0
24	1120.0
25	1120.0
26	1658.5
27	2197.0
28	2197.0
29	2197.0
30	2987.5
31	3778.0
32	3778.0
33	3778.0
34	4237.0
35	4696.0
36	4696.0
37	4696.0
38	5342.0
39	5988.0
40	5988.0
41	5988.0
42	6337.0
43	6686.0
44	6686.0
45	6686.0
46	6752.5
47	6819.0
48	6819.0
49	6819.0
50	6534.5
51	6250.0
52	6250.0
53	6250.0
54	5829.5
55	5409.0
56	5409.0
57	5409.0
58	4843.0
59	4277.0
60	4277.0
61	4277.0
62	3702.5
63	3128.0
64	3128.0
65	3128.0
66	2387.0
67	1646.0
68	1646.0
69	1646.0
70	1119.0
71	592.0
72	592.0
73	592.0
74	405.5
75	219.0
76	219.0
77	219.0
78	136.5
79	54.0
80	54.0
81	54.0
82	34.5
83	15.0
84	15.0
85	15.0
86	10.0
87	5.0
88	5.0
89	5.0
90	3.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	53650.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.04846225535881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4321706328878	72.38956197576887
2	5.839891957341778	9.349487418452936
3	1.6741955013272483	4.020503261882572
4	0.7078656917990034	2.266542404473439
5	0.3655753737251432	1.4631873252562908
6	0.22819354538257344	1.0959925442684064
7	0.14436734503795465	0.8089468779123952
8	0.11176826712615844	0.7157502329916122
9	0.06752666138872072	0.48648648648648646
>10	0.39817445163693943	5.949673811742777
>50	0.03027057234666791	1.4538676607642125
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAGGACCTGGAATATGGCGAGA	71	0.13233923578751167	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	69	0.12861136999068032	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	66	0.12301957129543337	No Hit
GATATACACTGTTCTACAAATCCCG	65	0.12115563839701769	No Hit
CCCTAGAGGTGGAGCCCAGTGACAC	64	0.11929170549860205	No Hit
CTGTAGGACCTGGAATATGGCGAGA	64	0.11929170549860205	No Hit
CCCTCTCTGATTACAACATCCAGAA	62	0.11556383970177074	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	58	0.10810810810810811	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	55	0.10251630941286113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGACA	30	7.5907505E-4	19.0	18
GCCCAGT	45	3.4373013E-5	16.88889	14
GAGGTGG	35	0.0021336984	16.285715	6
GTGACAC	35	0.0021336984	16.285715	19
GAGCCCA	45	6.613065E-4	14.777779	12
GGTGGAG	40	0.005191727	14.25	8
CAGTGAC	40	0.005191727	14.25	17
GGAGCCC	40	0.005191727	14.25	11
AGCCCAG	50	0.0014669098	13.299999	13
TATGGCG	85	5.1042905E-5	11.17647	16
CTGGAAT	60	0.005751345	11.083334	10
TGGCGAG	90	9.127852E-5	10.555556	18
ATGGCGA	95	1.5777622E-4	10.0	17
ATATGGC	120	1.6810358E-4	8.708334	15
AATATGG	130	3.9927382E-4	8.038462	14
>>END_MODULE