FastQCFastQC Report
Mon 15 Aug 2016
SRR2920818_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920818_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160848
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAG250.006009104719.01
TCAGAAC456.7158113E-414.7777783
CTCAAGA400.00525191714.252
CTGACCA400.00525191714.254
CTGAAGT550.003047927512.0909094
GATGCCC550.003047927512.09090911
GCTCAAG550.003047927512.0909091
GGACAGT750.002625564810.1333346
CTTCTGG950.00180502819.01
TAGAACA850.00737454848.9411764
AGAACAG850.00737454848.9411765
AATATGG1250.00249498367.614
Produced by FastQC (version 0.11.5)