##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920818_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160848
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375248681985475	32.0	32.0	33.0	29.0	34.0
2	31.749496418979408	33.0	32.0	33.0	29.0	34.0
3	31.93071098179648	33.0	32.0	33.0	30.0	34.0
4	32.01595294936835	33.0	32.0	33.0	31.0	34.0
5	32.00452601213568	33.0	32.0	33.0	31.0	34.0
6	35.36389013229881	37.0	34.0	37.0	33.0	37.0
7	35.389417338108025	37.0	35.0	37.0	33.0	37.0
8	35.34688650154183	37.0	34.0	37.0	33.0	37.0
9	35.44284666268776	37.0	34.0	37.0	33.0	37.0
10	35.41142569382274	37.0	34.0	37.0	33.0	37.0
11	35.36495946483637	37.0	34.0	37.0	33.0	37.0
12	35.36790634636427	37.0	34.0	37.0	33.0	37.0
13	37.06733686461753	38.0	38.0	38.0	37.0	38.0
14	37.05544364866209	38.0	38.0	38.0	37.0	38.0
15	36.99573510394907	38.0	38.0	38.0	36.0	38.0
16	37.02512931463245	38.0	38.0	38.0	36.0	38.0
17	37.02903362180444	38.0	38.0	38.0	36.0	38.0
18	37.03991967571869	38.0	38.0	38.0	36.0	38.0
19	37.006503033920225	38.0	38.0	38.0	36.0	38.0
20	37.040472993136376	38.0	38.0	38.0	37.0	38.0
21	37.0629041082264	38.0	38.0	38.0	37.0	38.0
22	37.09941062369442	38.0	38.0	38.0	37.0	38.0
23	37.64952004376803	39.0	38.0	39.0	37.0	39.0
24	37.55839674723963	39.0	38.0	39.0	36.0	39.0
25	37.45252039192281	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	9.0
19	17.0
20	64.0
21	126.0
22	191.0
23	329.0
24	454.0
25	691.0
26	882.0
27	1016.0
28	1329.0
29	1559.0
30	1971.0
31	2484.0
32	3234.0
33	4674.0
34	8803.0
35	26151.0
36	99306.0
37	7556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2812344573759	17.89639908485029	11.89135084054511	26.931015617228688
2	17.571247388839154	19.397194867203822	37.0648065254153	25.966751218541727
3	19.472421167810605	22.723938127922015	29.17474883119467	28.628891873072714
4	13.740301402566399	16.04309658808316	35.53603401969561	34.68056798965483
5	14.537948870983787	34.97525614244504	33.66905401372725	16.81774097284393
6	33.92581816373222	33.81328956530389	17.555704764746842	14.70518750621705
7	28.58350741072317	29.98172187406744	22.083582015318807	19.351188699890578
8	26.277603700387942	33.97741967571869	20.67479856759176	19.0701780563016
9	26.11782552471899	15.947975728638216	19.357405749527505	38.57679299711529
10	15.93367651447329	26.728961504028646	33.38804337013826	23.949318611359793
11	35.51427434596638	20.915398388540734	24.35218342783249	19.2181438376604
12	24.380160151198645	22.979458867999604	30.10917139162439	22.531209589177358
13	28.694170894260417	20.608897841440367	25.385457077489303	25.311474186809907
14	23.423356212075998	19.929996021088233	25.18464637421665	31.462001392619122
15	24.477146125534667	28.004700089525514	22.273823734208694	25.244330050731122
16	25.11936735302895	25.876603998806324	24.365239232070028	24.638789416094696
17	24.2527106336417	25.45695314831394	25.868521834278326	24.42181438376604
18	24.113448721774596	26.047572863821745	26.06622401273252	23.772754401671143
19	25.32701681090222	25.366805928578533	25.277280413806825	24.028896846712424
20	25.226922311747735	25.540883318412416	25.242464935840047	23.989729433999802
21	24.355913657614643	25.201432408236347	25.32950363075699	25.113150303392022
22	24.69536456779071	25.357480354123147	25.144235551576642	24.802919526509502
23	24.63381577638516	24.730180045757486	25.437058589475782	25.198945588381576
24	24.64127623594947	25.564508107032722	25.530936038993335	24.26327961802447
25	25.065279021187703	24.989431015617228	25.50420272555456	24.441087237640506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	3.0
3	6.0
4	6.0
5	6.0
6	21.0
7	36.0
8	36.0
9	36.0
10	84.0
11	132.0
12	132.0
13	132.0
14	271.5
15	411.0
16	411.0
17	411.0
18	685.0
19	959.0
20	959.0
21	959.0
22	1661.0
23	2363.0
24	2363.0
25	2363.0
26	3310.5
27	4258.0
28	4258.0
29	4258.0
30	5704.0
31	7150.0
32	7150.0
33	7150.0
34	8594.0
35	10038.0
36	10038.0
37	10038.0
38	12090.5
39	14143.0
40	14143.0
41	14143.0
42	16228.5
43	18314.0
44	18314.0
45	18314.0
46	19738.5
47	21163.0
48	21163.0
49	21163.0
50	21977.5
51	22792.0
52	22792.0
53	22792.0
54	21832.5
55	20873.0
56	20873.0
57	20873.0
58	18611.5
59	16350.0
60	16350.0
61	16350.0
62	13855.5
63	11361.0
64	11361.0
65	11361.0
66	8868.5
67	6376.0
68	6376.0
69	6376.0
70	4561.0
71	2746.0
72	2746.0
73	2746.0
74	1876.0
75	1006.0
76	1006.0
77	1006.0
78	643.0
79	280.0
80	280.0
81	280.0
82	178.5
83	77.0
84	77.0
85	77.0
86	44.5
87	12.0
88	12.0
89	12.0
90	7.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	160848.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.292370370406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.5313243191618	56.210208136155394
2	8.487696858436825	11.42314481179325
3	3.0685737147578935	6.194747967671423
4	1.6006309981853217	4.308410158249574
5	0.8921391723574988	3.001707980411415
6	0.5564566588093101	2.2467172547804846
7	0.43357611751244857	2.0423455278387257
8	0.2727333295104384	1.4682297777416322
9	0.18881537944051174	1.1435251000445694
>10	0.9350856872155944	10.357607988831749
>50	0.029970695102135483	1.3707511318768713
>100	0.0029970695102135483	0.23260416460490313
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTCAG	25	0.0060091047	19.0	1
TCAGAAC	45	6.7158113E-4	14.777778	3
CTCAAGA	40	0.005251917	14.25	2
CTGACCA	40	0.005251917	14.25	4
CTGAAGT	55	0.0030479275	12.090909	4
GATGCCC	55	0.0030479275	12.090909	11
GCTCAAG	55	0.0030479275	12.090909	1
GGACAGT	75	0.0026255648	10.133334	6
CTTCTGG	95	0.0018050281	9.0	1
TAGAACA	85	0.0073745484	8.941176	4
AGAACAG	85	0.0073745484	8.941176	5
AATATGG	125	0.0024949836	7.6	14
>>END_MODULE