FastQCFastQC Report
Mon 15 Aug 2016
SRR2920818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160848
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT400.00525191714.256
ATCAGGA400.00525191714.252
ACTGTTC400.00525191714.258
CCACAAC550.003047927512.0909093
TTTATTA550.003047927512.0909092
ACACTGT700.00147884710.8571426
CACTGTT750.002625564810.1333347
TACACTG951.6231182E-410.05
TGTTCTA950.00180502819.010
GTTCTAC1000.00287540168.54999911
TTCTACA1200.00167014477.91666612
Produced by FastQC (version 0.11.5)