FastQCFastQC Report
Mon 15 Aug 2016
SRR2920817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66127
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGGACCTGGAATATGGCGAGA790.11946708606166921No Hit
GTCCTAAAGTGTGTATTTCTCATTT680.10283242850877855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATATG500.001473233313.29999913
ATGGCGA701.06076724E-412.21428617
CGTGAAA550.003015357812.0909099
TGGCGAG752.0171748E-411.418
TATGGCG750.00259069710.13333316
ATATGGC900.00108412379.515
AATATGG1151.0680871E-49.08695614
Produced by FastQC (version 0.11.5)