##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920817_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66127
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203850167102697	32.0	32.0	33.0	29.0	34.0
2	31.519938905439535	32.0	32.0	33.0	29.0	34.0
3	31.982624344065208	33.0	32.0	33.0	31.0	34.0
4	32.1064618083385	33.0	32.0	33.0	32.0	34.0
5	32.061094560466984	33.0	32.0	33.0	32.0	34.0
6	35.249429128797615	37.0	34.0	37.0	32.0	37.0
7	34.92443328746201	37.0	34.0	37.0	32.0	37.0
8	34.95032286358069	37.0	34.0	37.0	32.0	37.0
9	35.168221755107595	37.0	34.0	37.0	32.0	37.0
10	35.31831173348254	37.0	34.0	37.0	33.0	37.0
11	35.267530660698355	37.0	34.0	37.0	33.0	37.0
12	35.323876782554784	37.0	34.0	37.0	33.0	37.0
13	36.95682550244227	38.0	38.0	38.0	36.0	38.0
14	36.899813994283726	38.0	38.0	38.0	36.0	38.0
15	36.88942489452115	38.0	38.0	38.0	36.0	38.0
16	36.90616540898574	38.0	38.0	38.0	36.0	38.0
17	36.86905500022684	38.0	38.0	38.0	36.0	38.0
18	36.895640207479545	38.0	38.0	38.0	36.0	38.0
19	36.86829887942898	38.0	38.0	38.0	36.0	38.0
20	36.945665159465875	38.0	38.0	38.0	36.0	38.0
21	36.92756362756514	38.0	38.0	38.0	36.0	38.0
22	36.918928728053594	38.0	38.0	38.0	36.0	38.0
23	37.427994616419916	39.0	38.0	39.0	36.0	39.0
24	37.37896774388676	39.0	38.0	39.0	35.0	39.0
25	37.166029004793806	39.0	38.0	39.0	34.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	6.0
20	19.0
21	39.0
22	72.0
23	116.0
24	146.0
25	223.0
26	308.0
27	424.0
28	511.0
29	675.0
30	919.0
31	1202.0
32	1649.0
33	2571.0
34	5056.0
35	13601.0
36	36296.0
37	2292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.931434966050176	18.594522660940314	12.4336503999879	27.04039197302161
2	16.778320504483794	20.15364374612488	38.161416667926865	24.906619081464456
3	18.555204379451663	23.672629939359112	30.11326689551923	27.65889878567
4	12.751221135088542	17.18965021851891	36.88054803635429	33.17858061003826
5	13.584466254328792	34.9796603505376	34.2205150694875	17.215358325646104
6	31.324572413688813	34.99780724968621	19.199419299227245	14.478201037397733
7	27.078198012914545	30.402105040301237	23.33388782191843	19.185809124865788
8	25.13194307922634	35.084005020642095	21.35587581472016	18.428176085411405
9	25.429854673582653	17.015742435011415	20.554387768989972	37.00001512241596
10	15.4959396313155	27.501625659715394	34.138854023318764	22.86358068565034
11	33.813722080239536	21.488953075143286	25.85177007878779	18.84555476582939
12	24.236696054561676	23.5667730276589	31.04027099369395	21.15625992408547
13	27.459282895035308	21.96228469460281	26.406762744416046	24.17166966594583
14	22.809139988204517	20.983864382173696	26.192024437824184	30.014971191797603
15	23.38984076095997	28.96245104117834	23.336912305109863	24.310795892751827
16	23.672629939359112	26.662331574092278	26.247977376865727	23.417061109682884
17	23.25071453415398	26.260075309631464	26.957218685257157	23.5319914709574
18	23.149394347240914	26.464227925053308	27.77836587173167	22.608011855974112
19	24.25333071211457	25.741376442300425	26.311491523885856	23.693801321699155
20	23.9856639496726	26.56252362877493	26.37198118771455	23.07983123383792
21	23.172077971176673	26.126998049208343	26.2283182361214	24.47260574349358
22	23.509307847021642	26.60940311824217	26.356858771757373	23.52443026297881
23	23.418573351278603	25.977286131232326	26.583695011114976	24.0204455063741
24	23.409499901704297	26.70618658036808	26.71223554675095	23.172077971176673
25	23.83141530690943	25.812451797299136	26.961755410044308	23.39437748574712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	7.0
7	13.0
8	13.0
9	13.0
10	42.0
11	71.0
12	71.0
13	71.0
14	145.5
15	220.0
16	220.0
17	220.0
18	378.0
19	536.0
20	536.0
21	536.0
22	871.5
23	1207.0
24	1207.0
25	1207.0
26	1835.0
27	2463.0
28	2463.0
29	2463.0
30	3295.5
31	4128.0
32	4128.0
33	4128.0
34	4716.0
35	5304.0
36	5304.0
37	5304.0
38	6058.0
39	6812.0
40	6812.0
41	6812.0
42	7416.5
43	8021.0
44	8021.0
45	8021.0
46	8428.5
47	8836.0
48	8836.0
49	8836.0
50	8600.0
51	8364.0
52	8364.0
53	8364.0
54	7830.0
55	7296.0
56	7296.0
57	7296.0
58	6505.5
59	5715.0
60	5715.0
61	5715.0
62	4765.0
63	3815.0
64	3815.0
65	3815.0
66	2915.5
67	2016.0
68	2016.0
69	2016.0
70	1455.5
71	895.0
72	895.0
73	895.0
74	606.0
75	317.0
76	317.0
77	317.0
78	196.0
79	75.0
80	75.0
81	75.0
82	48.0
83	21.0
84	21.0
85	21.0
86	11.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	66127.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.25919820950595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.65575716466	71.95699184901781
2	6.020010174665083	9.663223796633751
3	2.001017466508394	4.81800172395542
4	0.8648465321349839	2.776475569737021
5	0.43524956192414227	1.7466390430535181
6	0.26567181052512573	1.2793563899768627
7	0.17334614587455013	0.9738835876419617
8	0.1413147928325137	0.9073449574303991
9	0.08478887569950823	0.6124578462655194
>10	0.3429238972735666	4.532188062364844
>50	0.015073577902134795	0.733437173922906
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAAGGACCTGGAATATGGCGAGA	79	0.11946708606166921	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	68	0.10283242850877855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATATG	50	0.0014732333	13.299999	13
ATGGCGA	70	1.06076724E-4	12.214286	17
CGTGAAA	55	0.0030153578	12.090909	9
TGGCGAG	75	2.0171748E-4	11.4	18
TATGGCG	75	0.002590697	10.133333	16
ATATGGC	90	0.0010841237	9.5	15
AATATGG	115	1.0680871E-4	9.086956	14
>>END_MODULE