##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66127
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16096299544815	33.0	32.0	33.0	32.0	34.0
2	32.31645167631981	33.0	32.0	33.0	32.0	34.0
3	32.52594250457453	33.0	32.0	34.0	32.0	34.0
4	32.4700198103649	33.0	32.0	34.0	32.0	34.0
5	32.32864034358129	33.0	32.0	34.0	32.0	34.0
6	35.45141923873758	37.0	35.0	37.0	33.0	37.0
7	35.37459736567514	37.0	35.0	37.0	33.0	37.0
8	35.31046319960077	37.0	34.0	37.0	33.0	37.0
9	35.36891133727524	37.0	34.0	37.0	33.0	37.0
10	35.35985301011689	37.0	34.0	37.0	33.0	37.0
11	35.33078772664721	37.0	34.0	37.0	33.0	37.0
12	35.271568345758915	37.0	34.0	37.0	33.0	37.0
13	36.933960409515024	38.0	38.0	38.0	36.0	38.0
14	36.97144887867286	38.0	38.0	38.0	36.0	38.0
15	36.97002737157288	38.0	38.0	38.0	36.0	38.0
16	36.96812194716228	38.0	38.0	38.0	36.0	38.0
17	36.845660622741086	38.0	38.0	38.0	35.0	38.0
18	36.891224462020055	38.0	38.0	38.0	36.0	38.0
19	36.92552210141092	38.0	38.0	38.0	36.0	38.0
20	36.93521557003947	38.0	38.0	38.0	36.0	38.0
21	36.94130990367021	38.0	38.0	38.0	36.0	38.0
22	36.95368003992318	38.0	38.0	38.0	36.0	38.0
23	37.54284936561465	39.0	38.0	39.0	36.0	39.0
24	37.518653500083175	39.0	38.0	39.0	36.0	39.0
25	37.29142407791069	39.0	38.0	39.0	35.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	7.0
24	33.0
25	58.0
26	125.0
27	236.0
28	416.0
29	634.0
30	964.0
31	1341.0
32	1967.0
33	2992.0
34	4902.0
35	11644.0
36	35439.0
37	5367.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.247493459555095	18.570326795408835	12.628729565835437	26.553450179200627
2	17.483025088088073	20.3033556641009	38.12209838643822	24.09152086137281
3	18.851603732212258	24.26089192009315	29.854673582651564	27.032830765043027
4	13.431729853161341	18.326855898498344	35.92027462307378	32.32113962526653
5	13.480121584224294	35.65865682701468	33.91353002555688	16.94769156320414
6	31.255009300285813	35.66773027658899	18.960485127103908	14.116775296021292
7	27.029806281851588	30.851240794229284	23.18115142075098	18.937801503168146
8	25.11379618007773	35.7388056315877	21.056451978768127	18.090946209566443
9	25.266532581245183	17.26375005670906	20.67687933824308	36.79283802380268
10	15.872487788649114	27.932614514494837	33.682157061412134	22.51274063544392
11	33.92562795832262	21.537344806206242	25.832110938043463	18.704916297427676
12	24.02649447275697	23.4972099142559	31.03119754411965	21.445098068867484
13	27.53943169960833	21.794425877478186	26.28124669197151	24.384895730941974
14	22.762260498737277	21.505587732696174	25.981822856019477	29.75032891254707
15	23.392865244151405	29.030501912985617	23.06622105947646	24.510411783386512
16	23.746729777549263	27.10995508642461	26.04836148623104	23.09495364979509
17	23.28852057404691	26.674429506858015	26.612427601433602	23.42462231766147
18	23.234079876601086	26.616964326220753	27.621092745777066	22.52786305140109
19	24.276014336050327	26.20714685378136	26.20563461218564	23.31120419798267
20	23.950882392971103	27.02375731546872	26.63662346696508	22.3887368245951
21	23.708923737656328	26.057434935805347	25.927382158573653	24.306259167964676
22	23.62423820829616	26.56252362877493	26.509595172924826	23.303642990004082
23	23.303642990004082	26.396177053246028	26.55798690398778	23.742193052762108
24	23.373206103407078	26.93755954451283	26.68955192281519	22.9996824292649
25	23.879807037972387	26.182950988249882	26.975365584405765	22.961876389371966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	9.0
7	16.0
8	16.0
9	16.0
10	57.5
11	99.0
12	99.0
13	99.0
14	164.5
15	230.0
16	230.0
17	230.0
18	379.0
19	528.0
20	528.0
21	528.0
22	858.5
23	1189.0
24	1189.0
25	1189.0
26	1869.5
27	2550.0
28	2550.0
29	2550.0
30	3290.5
31	4031.0
32	4031.0
33	4031.0
34	4807.5
35	5584.0
36	5584.0
37	5584.0
38	6206.0
39	6828.0
40	6828.0
41	6828.0
42	7488.0
43	8148.0
44	8148.0
45	8148.0
46	8432.0
47	8716.0
48	8716.0
49	8716.0
50	8628.0
51	8540.0
52	8540.0
53	8540.0
54	7906.0
55	7272.0
56	7272.0
57	7272.0
58	6368.0
59	5464.0
60	5464.0
61	5464.0
62	4578.5
63	3693.0
64	3693.0
65	3693.0
66	2853.0
67	2013.0
68	2013.0
69	2013.0
70	1434.5
71	856.0
72	856.0
73	856.0
74	572.5
75	289.0
76	289.0
77	289.0
78	174.5
79	60.0
80	60.0
81	60.0
82	38.0
83	16.0
84	16.0
85	16.0
86	9.5
87	3.0
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	66127.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.88416229376806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.32702318977756	71.35814417711373
2	6.28679602460956	10.044308678754518
3	1.9725508755324184	4.727267228212379
4	0.9181258873639375	2.933748695691624
5	0.441079034548036	1.7617614590106916
6	0.2801703738760057	1.3428705369969907
7	0.18930430667297682	1.0585691170021323
8	0.14387127307146239	0.9194428901961378
9	0.07572172266919072	0.5444069744582394
>10	0.3521060104117369	4.709120329063771
>50	0.013251301467108378	0.6003599134997807
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGAGA	35	0.0021407832	16.285715	19
TGGCGAG	50	0.0014732333	13.299999	18
ATGGCGA	55	0.0030153578	12.090909	17
TATGGCG	55	0.0030153578	12.090909	16
>>END_MODULE