FastQCFastQC Report
Mon 15 Aug 2016
SRR2920816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270899
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC250.006019507519.0000021
CCCTAGA851.6958438E-814.5294121
AGAAGTG400.005264224514.2500015
CCTAGAG903.5950507E-813.7222222
TAGAGGT852.6713133E-713.4117654
GTATTGG500.001493979113.3000011
GATTAAG500.001493979113.3000011
GACCAGC655.420141E-513.1538466
GTCTTGA604.0726757E-412.6666661
GTGACAC1501.8189894E-1212.66666619
CTAGAGG853.9094775E-612.2941183
AGGGTGT550.003057214712.0909098
CAAGGCC550.003057214712.0909094
GAGCCCA1650.012.09090912
TAGCACT657.9859584E-411.6923074
AGTGACA1657.2759576E-1211.51515118
CCAGTGA1657.2759576E-1211.51515116
GTTGGGC600.00585445911.08333315
TATGGCG700.001484526210.85714316
TGGCGAG700.001484526210.85714318
Produced by FastQC (version 0.11.5)