##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920816_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270899
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3630799670726	32.0	32.0	33.0	29.0	34.0
2	31.739253374874032	33.0	32.0	33.0	29.0	34.0
3	31.85322574095881	33.0	32.0	34.0	30.0	34.0
4	31.968744808950937	33.0	32.0	34.0	31.0	34.0
5	32.005031395464734	33.0	32.0	34.0	31.0	34.0
6	34.94685104042466	37.0	34.0	37.0	32.0	37.0
7	34.88792871143858	37.0	34.0	37.0	32.0	37.0
8	34.7446022318281	37.0	34.0	37.0	32.0	37.0
9	34.9577591648548	37.0	34.0	37.0	32.0	37.0
10	34.944248594494624	37.0	34.0	37.0	32.0	37.0
11	34.891753014961296	37.0	34.0	37.0	32.0	37.0
12	34.908748278878846	37.0	34.0	37.0	32.0	37.0
13	36.596192676975555	38.0	38.0	38.0	34.0	38.0
14	36.59133846931882	38.0	38.0	38.0	34.0	38.0
15	36.51336106814717	38.0	38.0	38.0	34.0	38.0
16	36.558503353648405	38.0	38.0	38.0	34.0	38.0
17	36.56730737285852	38.0	38.0	38.0	34.0	38.0
18	36.578872568743336	38.0	38.0	38.0	34.0	38.0
19	36.55319141082101	38.0	38.0	38.0	34.0	38.0
20	36.59149350865083	38.0	38.0	38.0	34.0	38.0
21	36.62510382098125	38.0	38.0	38.0	34.0	38.0
22	36.654967349454964	38.0	38.0	38.0	34.0	38.0
23	37.155600426727304	39.0	38.0	39.0	35.0	39.0
24	37.055020505797366	39.0	38.0	39.0	34.0	39.0
25	36.94359890586529	39.0	38.0	39.0	34.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	2.0
17	3.0
18	15.0
19	62.0
20	185.0
21	372.0
22	738.0
23	1089.0
24	1489.0
25	1959.0
26	2531.0
27	2895.0
28	3555.0
29	4116.0
30	4686.0
31	5745.0
32	7039.0
33	9808.0
34	16112.0
35	42168.0
36	149891.0
37	16435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.03264685362441	16.977175995481712	12.028099033219023	26.962078117674853
2	18.089398631962467	18.999331854307325	36.33863543239362	26.572634081336588
3	19.466295556646575	22.13850918608042	28.884565834499202	29.5106294227738
4	14.049885750777966	15.679644443131943	35.21792254677943	35.05254725931067
5	15.259930822926627	34.30466705303453	33.104588795085995	17.330813328952857
6	34.123049549832224	33.54386690242489	17.456321359621114	14.876762188121774
7	29.40948471570585	29.561570917574446	21.75607883380891	19.272865532910792
8	26.8314021092732	33.105327077619336	20.16618739825544	19.89708341485203
9	26.215305335198728	15.71212887459902	19.44082480924625	38.631740980956
10	16.370676894340697	26.3367528119336	32.61141606281308	24.68115423091263
11	35.8210993765204	20.924403559998375	23.74722682623413	19.50727023724709
12	24.69038276257941	22.628728788219966	29.52724077977401	23.153647669426615
13	28.94953469743336	20.16397255065541	24.929586303382443	25.956906448528787
14	23.91186383116955	19.52314331171396	24.82401190111444	31.74098095600205
15	25.14885621578522	27.309809190879257	21.938803760811222	25.6025308325243
16	25.76126157719298	25.30869438425391	23.69185563623343	25.238188402319683
17	24.746123093846784	24.929586303382443	25.453397760789077	24.8708928419817
18	24.674509688112543	25.399503135855063	25.492157593789567	24.43382958224283
19	25.579644073990675	24.790050904580674	25.070967408517564	24.559337612911087
20	25.567462412190523	24.911867522582217	24.96465472371622	24.556015341511042
21	24.872738548315056	24.61286309657843	25.009689958250124	25.50470839685639
22	25.14590308565185	24.528698887777363	25.040697824650515	25.284700201920273
23	25.066537713317512	24.53866570197749	24.93696912871587	25.457827455989136
24	24.726927747979875	25.324936599987446	25.121539762051537	24.82659588998114
25	25.281377930520232	24.6763553944459	24.97535982044969	25.066906854584182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	1.0
5	1.0
6	20.5
7	40.0
8	40.0
9	40.0
10	92.5
11	145.0
12	145.0
13	145.0
14	282.5
15	420.0
16	420.0
17	420.0
18	817.0
19	1214.0
20	1214.0
21	1214.0
22	2072.5
23	2931.0
24	2931.0
25	2931.0
26	4235.0
27	5539.0
28	5539.0
29	5539.0
30	7428.0
31	9317.0
32	9317.0
33	9317.0
34	11924.5
35	14532.0
36	14532.0
37	14532.0
38	18790.0
39	23048.0
40	23048.0
41	23048.0
42	26960.0
43	30872.0
44	30872.0
45	30872.0
46	33902.5
47	36933.0
48	36933.0
49	36933.0
50	38808.5
51	40684.0
52	40684.0
53	40684.0
54	38983.0
55	37282.0
56	37282.0
57	37282.0
58	33393.5
59	29505.0
60	29505.0
61	29505.0
62	24897.5
63	20290.0
64	20290.0
65	20290.0
66	15728.5
67	11167.0
68	11167.0
69	11167.0
70	7931.5
71	4696.0
72	4696.0
73	4696.0
74	3207.0
75	1718.0
76	1718.0
77	1718.0
78	1084.0
79	450.0
80	450.0
81	450.0
82	275.0
83	100.0
84	100.0
85	100.0
86	55.5
87	11.0
88	11.0
89	11.0
90	6.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	270899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.65608078516497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.22470646502941	53.61414718844719
2	7.714918816421637	9.8220299085825
3	2.7458916214170475	5.243780966406937
4	1.4459259776988165	3.68167923383058
5	0.8905780236095794	2.834535330819197
6	0.6139936466097903	2.3450657502102503
7	0.44339427369012635	1.975731919398876
8	0.29555472551412976	1.5051084387011762
9	0.26728309691856356	1.5312774968961445
>10	1.29815206828354	14.462320809798484
>50	0.04977689720174407	2.069597535358294
>100	0.009824387605607382	0.9147254215503824
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAC	25	0.0060195075	19.000002	1
CCCTAGA	85	1.6958438E-8	14.529412	1
AGAAGTG	40	0.0052642245	14.250001	5
CCTAGAG	90	3.5950507E-8	13.722222	2
TAGAGGT	85	2.6713133E-7	13.411765	4
GTATTGG	50	0.0014939791	13.300001	1
GATTAAG	50	0.0014939791	13.300001	1
GACCAGC	65	5.420141E-5	13.153846	6
GTCTTGA	60	4.0726757E-4	12.666666	1
GTGACAC	150	1.8189894E-12	12.666666	19
CTAGAGG	85	3.9094775E-6	12.294118	3
AGGGTGT	55	0.0030572147	12.090909	8
CAAGGCC	55	0.0030572147	12.090909	4
GAGCCCA	165	0.0	12.090909	12
TAGCACT	65	7.9859584E-4	11.692307	4
AGTGACA	165	7.2759576E-12	11.515151	18
CCAGTGA	165	7.2759576E-12	11.515151	16
GTTGGGC	60	0.005854459	11.083333	15
TATGGCG	70	0.0014845262	10.857143	16
TGGCGAG	70	0.0014845262	10.857143	18
>>END_MODULE