FastQCFastQC Report
Mon 15 Aug 2016
SRR2920816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270899
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTG350.002163983416.28571514
TCAGGAC551.1214623E-515.5454553
TGGCGAG551.9507651E-413.81818218
GTTTTTA500.001493979113.3000011
CTAGAGG604.0726757E-412.6666663
ATGGCGA604.0726757E-412.66666617
GGACTGT604.0726757E-412.6666666
TATGGCG657.9859584E-411.69230716
TAGAGGT855.2851083E-511.1764714
CAAGAGG600.00585445911.0833334
TAGAAGA700.001484526210.8571434
GTGACAC1053.4312707E-610.85714319
CCTAGAG803.7490562E-410.6875012
CCCTAGA909.447783E-510.5555561
CAGGATA1002.3783725E-510.4500014
TAGATGA800.004495169.5000014
AGTGACA1304.18916E-69.518
AGAGGTG1151.1012921E-49.0869565
AGGACCT1054.461096E-49.047625
ACTGTAC950.00181336279.03
Produced by FastQC (version 0.11.5)