##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	270899
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.867618558946326	33.0	32.0	34.0	32.0	34.0
2	32.906957943735485	33.0	32.0	34.0	32.0	34.0
3	33.094928368137204	33.0	33.0	34.0	32.0	34.0
4	33.20707717636462	33.0	33.0	34.0	32.0	35.0
5	33.11798862306616	34.0	33.0	34.0	32.0	35.0
6	35.863857009438945	37.0	35.0	37.0	33.0	37.0
7	35.83336224939922	37.0	37.0	37.0	33.0	37.0
8	35.789552563870664	37.0	35.0	37.0	33.0	37.0
9	35.780866669865894	37.0	35.0	37.0	33.0	37.0
10	35.76374220650501	37.0	35.0	37.0	33.0	37.0
11	35.65729663084766	37.0	34.0	37.0	33.0	37.0
12	35.63944495919143	37.0	34.0	37.0	33.0	37.0
13	37.41231972063389	38.0	38.0	38.0	37.0	38.0
14	37.433818508004826	38.0	38.0	38.0	37.0	38.0
15	37.434741361171504	38.0	38.0	38.0	37.0	38.0
16	37.40703730910782	38.0	38.0	38.0	37.0	38.0
17	37.32420570027944	38.0	38.0	38.0	37.0	38.0
18	37.37221990483538	38.0	38.0	38.0	37.0	38.0
19	37.37451965492674	38.0	38.0	38.0	37.0	38.0
20	37.36746167390799	38.0	38.0	38.0	37.0	38.0
21	37.35503637887183	38.0	38.0	38.0	37.0	38.0
22	37.36798954591933	38.0	38.0	38.0	37.0	38.0
23	38.02250654302895	39.0	38.0	39.0	37.0	39.0
24	38.01934300237358	39.0	38.0	39.0	37.0	39.0
25	37.883576535904524	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	1.0
18	1.0
19	1.0
20	1.0
21	0.0
22	8.0
23	17.0
24	46.0
25	142.0
26	252.0
27	548.0
28	913.0
29	1481.0
30	2204.0
31	3158.0
32	4419.0
33	6506.0
34	11242.0
35	30273.0
36	164684.0
37	45000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67716381381991	17.48511437842148	12.079040528019668	26.758681279738944
2	18.293164611165047	19.45226818851306	36.064732612523485	26.189834587798405
3	19.744258930450094	22.219351123481445	28.814798135098318	29.22159181097014
4	14.428255549116093	16.049893133603298	34.79119524250736	34.73065607477325
5	15.166538082458775	34.630987932771994	33.06472153828548	17.137752446483745
6	34.18358871756632	33.669374933093145	17.253662804218546	14.893373545121982
7	29.542744712974205	29.6523796691756	21.554158560939683	19.25071705691051
8	26.718075740405094	33.802265789094825	19.99269100291991	19.48696746758017
9	26.05620544926338	16.128520223404294	19.142189524509135	38.67308480282319
10	16.27543844753949	26.607702501670367	32.51876160487857	24.598097445911577
11	35.89049793465461	21.119310148800842	23.66970716023315	19.320484756311394
12	24.906330403582146	22.547148568285596	29.39767219517237	23.148848832959885
13	28.83473176349857	20.28431260359027	24.998615720249983	25.88233991266118
14	23.998612028837314	19.43270370137948	24.859818603981555	31.708865665801646
15	25.132244858785008	27.46484852288122	22.00008121107867	25.402825407255104
16	25.83988866699397	25.569308118523875	23.674136855433208	24.916666359048946
17	24.62725960597861	25.216778208852748	25.5471596425236	24.608802542645044
18	24.65199207084559	25.447491500522336	25.496587288989623	24.40392913964245
19	25.458565738522477	24.780822372913892	25.21567078505273	24.544941103510904
20	25.71881033152577	24.996400872649954	24.739109409780028	24.545679386044245
21	24.87679910224844	24.60695683631169	25.01153566458348	25.50470839685639
22	25.19869028678585	24.592929468178177	25.18724690751904	25.021133337516932
23	25.16029959505203	24.38214980490884	24.921834336782343	25.535716263256784
24	24.743539104980083	25.382522637588178	25.18798519005238	24.685953067379355
25	25.410577373855205	24.715853509979734	25.095330732117876	24.778238384047192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.5
3	3.0
4	3.0
5	3.0
6	12.5
7	22.0
8	22.0
9	22.0
10	88.0
11	154.0
12	154.0
13	154.0
14	283.5
15	413.0
16	413.0
17	413.0
18	863.5
19	1314.0
20	1314.0
21	1314.0
22	2167.5
23	3021.0
24	3021.0
25	3021.0
26	4442.0
27	5863.0
28	5863.0
29	5863.0
30	7635.5
31	9408.0
32	9408.0
33	9408.0
34	12008.0
35	14608.0
36	14608.0
37	14608.0
38	18929.0
39	23250.0
40	23250.0
41	23250.0
42	26951.0
43	30652.0
44	30652.0
45	30652.0
46	33867.5
47	37083.0
48	37083.0
49	37083.0
50	39058.0
51	41033.0
52	41033.0
53	41033.0
54	39099.0
55	37165.0
56	37165.0
57	37165.0
58	33165.5
59	29166.0
60	29166.0
61	29166.0
62	24510.0
63	19854.0
64	19854.0
65	19854.0
66	15522.0
67	11190.0
68	11190.0
69	11190.0
70	7823.0
71	4456.0
72	4456.0
73	4456.0
74	3064.0
75	1672.0
76	1672.0
77	1672.0
78	1066.5
79	461.0
80	461.0
81	461.0
82	277.0
83	93.0
84	93.0
85	93.0
86	53.0
87	13.0
88	13.0
89	13.0
90	7.5
91	2.0
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	270899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.21499541153366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.26564934315141	51.803719918225354
2	8.096107013089794	10.07398521341334
3	2.911925527455239	5.434962999880663
4	1.5969113455475494	3.974073281434673
5	0.9873194212160982	3.0713036630338806
6	0.6352751202164819	2.371418321359795
7	0.4648336230882597	2.0243735209293847
8	0.3530816534781321	1.7573578760830963
9	0.2462867944396525	1.3790458607385883
>10	1.385577411806992	15.269873356566086
>50	0.047639117673372776	1.9590746645975026
>100	0.009393628837003083	0.8808113237376369
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGATTG	35	0.0021639834	16.285715	14
TCAGGAC	55	1.1214623E-5	15.545455	3
TGGCGAG	55	1.9507651E-4	13.818182	18
GTTTTTA	50	0.0014939791	13.300001	1
CTAGAGG	60	4.0726757E-4	12.666666	3
ATGGCGA	60	4.0726757E-4	12.666666	17
GGACTGT	60	4.0726757E-4	12.666666	6
TATGGCG	65	7.9859584E-4	11.692307	16
TAGAGGT	85	5.2851083E-5	11.176471	4
CAAGAGG	60	0.005854459	11.083333	4
TAGAAGA	70	0.0014845262	10.857143	4
GTGACAC	105	3.4312707E-6	10.857143	19
CCTAGAG	80	3.7490562E-4	10.687501	2
CCCTAGA	90	9.447783E-5	10.555556	1
CAGGATA	100	2.3783725E-5	10.450001	4
TAGATGA	80	0.00449516	9.500001	4
AGTGACA	130	4.18916E-6	9.5	18
AGAGGTG	115	1.1012921E-4	9.086956	5
AGGACCT	105	4.461096E-4	9.04762	5
ACTGTAC	95	0.0018133627	9.0	3
>>END_MODULE