FastQCFastQC Report
Mon 15 Aug 2016
SRR2920815_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920815_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84095
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG1370.16291099351923422No Hit
GTCCTTCAGTGTGCATTTCTCATTT950.11296747725786313No Hit
GTTTCTCATTGTAACTCATTGATAT890.1058326892205244No Hit
CTGAAGGACCTGGAATATGGCGAGA880.10464355788096795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCT350.002147319316.2857155
AAGTGTG750.002603298810.1333347
AAAGTGT800.00444090379.5000016
CTCATTT1356.566035E-69.14814919
ATTTCTC1606.1509E-68.31250115
GGAATAT1150.00108333128.2608712
TTCTCAT1855.1013158E-67.70270317
TCTCATT1802.963451E-57.38888918
TGGAATA1300.00361848067.30769211
AATATGG1350.00522625737.037037414
TTTCTCA1906.050974E-57.016
Produced by FastQC (version 0.11.5)