##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920815_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84095
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44571020869255	32.0	32.0	33.0	29.0	34.0
2	31.556977228134848	33.0	32.0	33.0	29.0	34.0
3	31.787573577501636	33.0	32.0	33.0	30.0	34.0
4	31.971817587252513	33.0	32.0	34.0	30.0	34.0
5	31.904572210000595	33.0	32.0	34.0	30.0	34.0
6	35.159843034663176	37.0	35.0	37.0	32.0	37.0
7	34.859920328200246	37.0	34.0	37.0	32.0	37.0
8	34.92005470004162	37.0	34.0	37.0	32.0	37.0
9	35.091705808906596	37.0	34.0	37.0	32.0	37.0
10	35.09073072120816	37.0	34.0	37.0	32.0	37.0
11	35.08933943754088	37.0	34.0	37.0	32.0	37.0
12	35.12752244485404	37.0	34.0	37.0	32.0	37.0
13	36.622641060705156	38.0	38.0	38.0	34.0	38.0
14	36.62348534395624	38.0	38.0	38.0	34.0	38.0
15	36.564492538200845	38.0	38.0	38.0	34.0	38.0
16	36.597621737320885	38.0	38.0	38.0	34.0	38.0
17	36.597039062964505	38.0	38.0	38.0	34.0	38.0
18	36.62930019620667	38.0	38.0	38.0	34.0	38.0
19	36.55079374516915	38.0	38.0	38.0	34.0	38.0
20	36.656329151554786	38.0	38.0	38.0	34.0	38.0
21	36.63630417979666	38.0	38.0	38.0	34.0	38.0
22	36.68064688744872	38.0	38.0	38.0	35.0	38.0
23	37.1938284083477	39.0	38.0	39.0	35.0	39.0
24	37.09611748617635	39.0	38.0	39.0	34.0	39.0
25	37.00706344015696	39.0	38.0	39.0	34.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	8.0
19	26.0
20	48.0
21	125.0
22	206.0
23	326.0
24	462.0
25	618.0
26	755.0
27	873.0
28	1038.0
29	1203.0
30	1404.0
31	1724.0
32	2204.0
33	3041.0
34	4944.0
35	12341.0
36	46548.0
37	6199.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.522147571199234	18.33402699328141	11.791426363041797	27.352399072477557
2	16.561032166002736	21.390094535941493	37.99036803614959	24.058505261906177
3	18.737142517391046	24.320114156608597	30.693858136631192	26.248885189369165
4	12.705868363160711	18.482668410725964	37.23883702954991	31.57262619656341
5	12.62619656341043	34.66436767941019	34.06504548427374	18.644390272905643
6	29.77822700517272	35.154289791307455	20.576728699684878	14.49075450383495
7	25.943278435103156	30.786610381116596	23.801652892561982	19.468458291218266
8	24.2927641357988	35.45632915155479	22.20346037219811	18.047446340448303
9	25.16796480171235	17.82032225459302	21.45906415363577	35.55264879005886
10	15.947440394791604	27.60568404780308	34.2053629823414	22.241512575063915
11	32.72370533325406	21.261668351269396	27.20970331173078	18.804923003745763
12	23.24989595100779	23.951483441346095	31.962661275937926	20.83595933170819
13	26.73167251322909	22.48528449967299	27.02776621677864	23.75527677031928
14	22.346156132944884	22.021523277245972	26.648433319460135	28.983887270349012
15	23.077471906772104	29.310898388727036	23.50437005767287	24.10725964682799
16	23.14168499910815	27.499851358582557	26.710268149117073	22.648195493192222
17	22.57209108746061	27.043224924192877	27.190677210297874	23.194006778048635
18	22.476960580296094	27.583090552351507	28.367917236458766	21.572031630893633
19	24.23568583150009	26.051489387002796	26.96117486176348	22.751649919733634
20	23.37356561032166	27.526012248052794	27.2418098578988	21.85861228372674
21	23.295082941910934	26.795885605565132	26.514061478090255	23.39496997443368
22	22.784945597241215	27.633034068612876	27.518877460015457	22.06314287413045
23	23.10125453356323	26.73642903858731	27.18235329092098	22.979963136928475
24	22.707652060170048	27.094357571793804	27.782864617396992	22.41512575063916
25	23.91343123848029	26.235804744634045	27.286996848801948	22.563767168083714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	3.5
3	5.0
4	5.0
5	5.0
6	12.0
7	19.0
8	19.0
9	19.0
10	48.0
11	77.0
12	77.0
13	77.0
14	156.5
15	236.0
16	236.0
17	236.0
18	416.0
19	596.0
20	596.0
21	596.0
22	1164.0
23	1732.0
24	1732.0
25	1732.0
26	2722.0
27	3712.0
28	3712.0
29	3712.0
30	4893.5
31	6075.0
32	6075.0
33	6075.0
34	6979.0
35	7883.0
36	7883.0
37	7883.0
38	8707.5
39	9532.0
40	9532.0
41	9532.0
42	10169.5
43	10807.0
44	10807.0
45	10807.0
46	10800.5
47	10794.0
48	10794.0
49	10794.0
50	10464.5
51	10135.0
52	10135.0
53	10135.0
54	9136.5
55	8138.0
56	8138.0
57	8138.0
58	7164.0
59	6190.0
60	6190.0
61	6190.0
62	5303.0
63	4416.0
64	4416.0
65	4416.0
66	3431.5
67	2447.0
68	2447.0
69	2447.0
70	1675.5
71	904.0
72	904.0
73	904.0
74	601.0
75	298.0
76	298.0
77	298.0
78	186.5
79	75.0
80	75.0
81	75.0
82	43.0
83	11.0
84	11.0
85	11.0
86	10.5
87	10.0
88	10.0
89	10.0
90	5.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	84095.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.6871395445627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.27002494987066	69.35132885427196
2	6.145627649967091	9.548724656638326
3	1.9913976519569574	4.64117961828884
4	0.8786028072431158	2.7302455556216185
5	0.4607307403835852	1.7896426660324631
6	0.3290933859882751	1.5339794280278258
7	0.2005173654161118	1.0904334383732683
8	0.12092268601429643	0.751531006599679
9	0.10561601689856272	0.7384505618645579
>10	0.468384074941452	6.172780783637553
>50	0.027552004408320705	1.4887924371246803
>100	0.0015306669115733725	0.16291099351923422
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	137	0.16291099351923422	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	95	0.11296747725786313	No Hit
GTTTCTCATTGTAACTCATTGATAT	89	0.1058326892205244	No Hit
CTGAAGGACCTGGAATATGGCGAGA	88	0.10464355788096795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCT	35	0.0021473193	16.285715	5
AAGTGTG	75	0.0026032988	10.133334	7
AAAGTGT	80	0.0044409037	9.500001	6
CTCATTT	135	6.566035E-6	9.148149	19
ATTTCTC	160	6.1509E-6	8.312501	15
GGAATAT	115	0.0010833312	8.26087	12
TTCTCAT	185	5.1013158E-6	7.702703	17
TCTCATT	180	2.963451E-5	7.388889	18
TGGAATA	130	0.0036184806	7.307692	11
AATATGG	135	0.0052262573	7.0370374	14
TTTCTCA	190	6.050974E-5	7.0	16
>>END_MODULE