FastQCFastQC Report
Mon 15 Aug 2016
SRR2920815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84095
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCG1050.12485879065342767No Hit
CTGAAGGACCTGGAATATGGCGAGA970.11534573993697604No Hit
GTTTCTCATTGTAACTCATTGATAT920.10940008323919376No Hit
GTCCTTCAGTGTGCATTTCTCATTT880.10464355788096795No Hit
TTGTAGAACAGTGTATATCAATGAG880.10464355788096795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGTT307.6399953E-418.99999811
AATGAGT402.7223397E-416.62500210
TGTGCAT456.668681E-414.77777810
ACAATGA456.668681E-414.77777818
GTGCATT551.9256755E-413.81818211
ATCAATG500.001479072113.2999997
GTGTGCA500.001479072113.2999999
TCAATGA550.003027137612.0909098
GCATTTC600.00579768311.08333313
ACACTGT909.2710325E-510.5555556
TGCATTT800.00444090379.50000112
CAATGAG800.00444090379.5000019
TGTTCTA1300.00361848067.30769210
GAATATG1400.00743542246.78571413
GGAATAT1400.00743542246.78571412
AATATGG2103.0378898E-56.785713714
ATATGGC1904.613673E-46.515
Produced by FastQC (version 0.11.5)