##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84095
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88062310482193	33.0	32.0	34.0	32.0	34.0
2	32.92152922290267	33.0	32.0	34.0	32.0	34.0
3	33.129222902669596	33.0	33.0	34.0	32.0	35.0
4	33.2292050656995	33.0	33.0	34.0	32.0	35.0
5	33.13393186277424	34.0	33.0	34.0	32.0	35.0
6	36.005434330221775	37.0	37.0	37.0	33.0	37.0
7	35.98504072774838	37.0	37.0	37.0	33.0	37.0
8	35.95288661632677	37.0	37.0	37.0	33.0	37.0
9	35.93685712586955	37.0	37.0	37.0	33.0	37.0
10	35.9174267197812	37.0	37.0	37.0	33.0	37.0
11	35.85699506510494	37.0	35.0	37.0	33.0	37.0
12	35.82528093227897	37.0	35.0	37.0	33.0	37.0
13	37.44913490695047	38.0	38.0	38.0	37.0	38.0
14	37.48187169272846	38.0	38.0	38.0	37.0	38.0
15	37.472608359593316	38.0	38.0	38.0	37.0	38.0
16	37.440002378262676	38.0	38.0	38.0	37.0	38.0
17	37.358404185742316	38.0	38.0	38.0	37.0	38.0
18	37.40450680777692	38.0	38.0	38.0	37.0	38.0
19	37.4013556097271	38.0	38.0	38.0	37.0	38.0
20	37.39756228075391	38.0	38.0	38.0	37.0	38.0
21	37.385242880076106	38.0	38.0	38.0	37.0	38.0
22	37.415220881146325	38.0	38.0	38.0	37.0	38.0
23	38.078744277305425	39.0	38.0	39.0	37.0	39.0
24	38.08782924073964	39.0	38.0	39.0	37.0	39.0
25	37.9584517509959	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	5.0
24	12.0
25	29.0
26	73.0
27	140.0
28	275.0
29	374.0
30	634.0
31	911.0
32	1305.0
33	1912.0
34	3264.0
35	8513.0
36	49908.0
37	16738.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.09881681431714	18.935727451096973	12.05541352042333	26.910042214162555
2	16.776264938462454	21.24026398715738	38.141387716273265	23.8420833581069
3	18.740709911409713	24.538914323086985	30.580890659373328	26.13948510612997
4	12.986503359296034	18.47672275402818	37.09851953148225	31.43825435519353
5	12.523931268208575	35.14002021523277	33.616742969260955	18.719305547297697
6	29.404839764551994	35.59664664962245	20.39954813009097	14.598965455734586
7	25.87549794874844	31.136214994946194	23.61614840359118	19.372138652714195
8	24.220227124085856	35.40638563529342	22.35091265830311	18.022474582317617
9	25.39508888756763	17.643141684999108	21.413877162732625	35.547892264700636
10	15.83090552351507	28.07539092692788	34.181580355550274	21.912123194006778
11	32.660681372257564	21.966823235626375	26.663892026874368	18.70860336524169
12	23.361674296926097	23.840894226767347	31.882989476187646	20.914442000118914
13	27.037279267495094	22.4876627623521	26.936203103632796	23.538854866520005
14	22.305725667399965	21.94541887151436	26.442713597716867	29.306141863368808
15	23.02396099649206	29.52732029252631	23.897972531066056	23.55074617991557
16	23.274867709138476	27.652060170045782	26.79350734288602	22.27956477792972
17	22.919317438611095	26.773292110113562	27.35715559783578	22.950234853439564
18	22.50193233842678	27.637790593971108	28.405969439324576	21.454307628277544
19	24.10369225280932	26.138295974790417	26.71978119983352	23.03823057256674
20	23.311730780664725	27.517688328675906	27.38093822462691	21.789642666032464
21	23.422319995243473	26.93144657827457	26.279802604197634	23.366430822284322
22	22.869373922349723	27.31672513229086	27.560497056899933	22.25340388845948
23	22.913371781913312	26.64605505678102	26.987335751233726	23.453237410071942
24	22.465069266900528	27.355966466496223	27.675842796836907	22.503121469766334
25	23.70176586003924	26.515250609429813	27.12646411796183	22.656519412569118
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	6.0
7	11.0
8	11.0
9	11.0
10	51.5
11	92.0
12	92.0
13	92.0
14	154.5
15	217.0
16	217.0
17	217.0
18	408.5
19	600.0
20	600.0
21	600.0
22	1176.0
23	1752.0
24	1752.0
25	1752.0
26	2710.5
27	3669.0
28	3669.0
29	3669.0
30	4873.5
31	6078.0
32	6078.0
33	6078.0
34	7000.5
35	7923.0
36	7923.0
37	7923.0
38	8806.5
39	9690.0
40	9690.0
41	9690.0
42	10283.0
43	10876.0
44	10876.0
45	10876.0
46	10900.5
47	10925.0
48	10925.0
49	10925.0
50	10447.5
51	9970.0
52	9970.0
53	9970.0
54	9033.0
55	8096.0
56	8096.0
57	8096.0
58	7186.0
59	6276.0
60	6276.0
61	6276.0
62	5285.0
63	4294.0
64	4294.0
65	4294.0
66	3310.5
67	2327.0
68	2327.0
69	2327.0
70	1617.5
71	908.0
72	908.0
73	908.0
74	591.0
75	274.0
76	274.0
77	274.0
78	186.5
79	99.0
80	99.0
81	99.0
82	56.0
83	13.0
84	13.0
85	13.0
86	8.5
87	4.0
88	4.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	84095.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.98674118556394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70439590991937	68.2906236993876
2	6.485743412313491	9.9863249895951
3	2.0851997157950017	4.815981925203639
4	0.9591918692657008	2.9538022474582317
5	0.49890333940873005	1.9204471133836731
6	0.3120076611782151	1.4412271835424222
7	0.2332334496926261	1.2569118259111718
8	0.1096660591269964	0.6754266008680658
9	0.1142998362732075	0.7919614721445983
>10	0.4649223070031819	6.110945953980617
>50	0.03089184764140743	1.631488197871455
>100	0.0015445923820703719	0.12485879065342767
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCG	105	0.12485879065342767	No Hit
CTGAAGGACCTGGAATATGGCGAGA	97	0.11534573993697604	No Hit
GTTTCTCATTGTAACTCATTGATAT	92	0.10940008323919376	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	88	0.10464355788096795	No Hit
TTGTAGAACAGTGTATATCAATGAG	88	0.10464355788096795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGAGTT	30	7.6399953E-4	18.999998	11
AATGAGT	40	2.7223397E-4	16.625002	10
TGTGCAT	45	6.668681E-4	14.777778	10
ACAATGA	45	6.668681E-4	14.777778	18
GTGCATT	55	1.9256755E-4	13.818182	11
ATCAATG	50	0.0014790721	13.299999	7
GTGTGCA	50	0.0014790721	13.299999	9
TCAATGA	55	0.0030271376	12.090909	8
GCATTTC	60	0.005797683	11.083333	13
ACACTGT	90	9.2710325E-5	10.555555	6
TGCATTT	80	0.0044409037	9.500001	12
CAATGAG	80	0.0044409037	9.500001	9
TGTTCTA	130	0.0036184806	7.307692	10
GAATATG	140	0.0074354224	6.785714	13
GGAATAT	140	0.0074354224	6.785714	12
AATATGG	210	3.0378898E-5	6.7857137	14
ATATGGC	190	4.613673E-4	6.5	15
>>END_MODULE