FastQCFastQC Report
Mon 15 Aug 2016
SRR2920814_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920814_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210510
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGGT350.002161828816.2857153
ATCCCCC508.6570435E-515.20000114
CTATGGT400.00525906814.254
CTTGGAC400.00525906814.253
GATAAAG551.9475131E-413.8181822
TCCCCCC551.9475131E-413.81818215
GGATAAA604.0659722E-412.6666671
GTGACAC905.331003E-712.66666719
GGCGAGA701.08228705E-412.21428619
TTTAGAA550.003053320612.0909092
GATTAAG550.003053320612.0909091
CCTAGAG657.972981E-411.6923072
CCCCCCT657.972981E-411.69230716
ACTGTAC657.972981E-411.6923073
AGCCCAG951.3446765E-511.013
CCCTAGA700.001482145310.8571431
AGAGGTG700.001482145310.8571435
GCATCCC700.001482145310.85714312
GGCATCC803.7415762E-410.687511
TGGCGAG1252.1994856E-710.63999918
Produced by FastQC (version 0.11.5)