##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920814_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	210510
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.078457080423732	32.0	32.0	33.0	29.0	34.0
2	31.56555508051874	33.0	32.0	33.0	29.0	34.0
3	31.950515414944658	33.0	32.0	34.0	30.0	34.0
4	32.06309914018336	33.0	32.0	34.0	31.0	34.0
5	32.28173958481782	33.0	32.0	34.0	32.0	34.0
6	35.088019571516796	37.0	34.0	37.0	32.0	37.0
7	34.961417509857014	37.0	34.0	37.0	32.0	37.0
8	35.0650277896537	37.0	34.0	37.0	32.0	37.0
9	35.1318749703102	37.0	34.0	37.0	33.0	37.0
10	35.109220464586	37.0	34.0	37.0	32.0	37.0
11	35.065593083463966	37.0	34.0	37.0	32.0	37.0
12	35.05840102607952	37.0	34.0	37.0	32.0	37.0
13	36.77476604436844	38.0	38.0	38.0	36.0	38.0
14	36.76929837062372	38.0	38.0	38.0	36.0	38.0
15	36.68601016578785	38.0	38.0	38.0	35.0	38.0
16	36.712070685478125	38.0	38.0	38.0	35.0	38.0
17	36.71796589235666	38.0	38.0	38.0	35.0	38.0
18	36.7261602774215	38.0	38.0	38.0	35.0	38.0
19	36.70814213101515	38.0	38.0	38.0	35.0	38.0
20	36.72912925751746	38.0	38.0	38.0	35.0	38.0
21	36.773792218896965	38.0	38.0	38.0	36.0	38.0
22	36.803919053726666	38.0	38.0	38.0	36.0	38.0
23	37.34349437081374	39.0	38.0	39.0	36.0	39.0
24	37.23323357560211	39.0	38.0	39.0	35.0	39.0
25	37.11134387915063	39.0	38.0	39.0	34.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	35.0
20	105.0
21	261.0
22	510.0
23	775.0
24	1066.0
25	1285.0
26	1733.0
27	1959.0
28	2338.0
29	2790.0
30	3424.0
31	4023.0
32	4987.0
33	7097.0
34	11935.0
35	32379.0
36	120698.0
37	13102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56035342739062	16.940287872310105	11.205643437366396	27.293715262932878
2	18.130255094769847	19.163935204978387	36.06622013206023	26.639589568191536
3	19.77815780723006	22.128639969597643	28.828559213339034	29.264643009833264
4	13.920478837109876	15.707567336468575	34.83444966984941	35.53750415657213
5	14.570804237328394	34.50002375184077	33.043560875967884	17.885611134862952
6	34.12854496223458	33.53522397985844	17.52173293430241	14.81449812360458
7	29.267018193910026	29.65179801434611	21.736734596931264	19.3444491948126
8	26.968790081231298	33.78509334473422	19.885991164315232	19.360125409719252
9	26.221557170680725	15.729419029974823	19.3078713600304	38.74115243931405
10	16.123699586717972	26.890884043513374	32.919101230345355	24.066315139423306
11	36.3293905277659	20.314949408579164	23.87677545009738	19.47888461355755
12	24.70903995059617	22.526720820863616	29.700726806327488	23.06351242221272
13	29.16346016816303	20.236568334045888	24.97126027267113	25.62871122511995
14	23.971307776352667	19.352999857488957	24.73564201225595	31.94005035390243
15	25.003087739299794	27.551660253669656	22.012730986651466	25.432521020379077
16	25.63773692461166	25.13039760581445	24.192674932307252	25.039190537266638
17	24.62400836064795	24.957009168210536	25.542254524725667	24.876727946415848
18	24.72234098142606	25.26150776685193	25.754595981188544	24.261555270533467
19	25.65056291862619	24.746567859009073	25.1745760296423	24.428293192722435
20	25.78879863189397	25.16840055104271	24.709514987411524	24.3332858296518
21	25.029689800959577	24.45584532801292	24.850125884756068	25.664338986271435
22	25.01686380694504	24.91568096527481	25.00213766566909	25.065317562111062
23	24.854401216094246	24.424017861384257	25.364590755783574	25.356990166737926
24	24.845375516602537	25.287634791696355	25.200703054486723	24.666286637214384
25	25.19500261270248	24.60595696166453	25.459123082038857	24.73991734359413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	9.5
7	17.0
8	17.0
9	17.0
10	56.5
11	96.0
12	96.0
13	96.0
14	185.0
15	274.0
16	274.0
17	274.0
18	549.5
19	825.0
20	825.0
21	825.0
22	1518.5
23	2212.0
24	2212.0
25	2212.0
26	3493.0
27	4774.0
28	4774.0
29	4774.0
30	6510.5
31	8247.0
32	8247.0
33	8247.0
34	10077.5
35	11908.0
36	11908.0
37	11908.0
38	14871.5
39	17835.0
40	17835.0
41	17835.0
42	20709.0
43	23583.0
44	23583.0
45	23583.0
46	25914.5
47	28246.0
48	28246.0
49	28246.0
50	29213.5
51	30181.0
52	30181.0
53	30181.0
54	29296.0
55	28411.0
56	28411.0
57	28411.0
58	25637.0
59	22863.0
60	22863.0
61	22863.0
62	19497.0
63	16131.0
64	16131.0
65	16131.0
66	12671.5
67	9212.0
68	9212.0
69	9212.0
70	6513.5
71	3815.0
72	3815.0
73	3815.0
74	2603.0
75	1391.0
76	1391.0
77	1391.0
78	887.5
79	384.0
80	384.0
81	384.0
82	234.5
83	85.0
84	85.0
85	85.0
86	50.0
87	15.0
88	15.0
89	15.0
90	9.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	210510.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.24075762637432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4212183298811	47.04973145471844
2	10.009093382068947	11.858925502137874
3	3.723961476680273	6.61830897749913
4	1.974124759553079	4.677945856196337
5	1.126781164483042	3.3375684931551852
6	0.8825572020801463	3.137001437990461
7	0.594521534491075	2.4653934289912143
8	0.404925195670065	1.9190460298802015
9	0.29574955242694123	1.5768384798089803
>10	1.5174452684027955	14.911097463838724
>50	0.03771282203953068	1.5450815267912936
>100	0.011909312223009687	0.9030613489921716
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAGGT	35	0.0021618288	16.285715	3
ATCCCCC	50	8.6570435E-5	15.200001	14
CTATGGT	40	0.005259068	14.25	4
CTTGGAC	40	0.005259068	14.25	3
GATAAAG	55	1.9475131E-4	13.818182	2
TCCCCCC	55	1.9475131E-4	13.818182	15
GGATAAA	60	4.0659722E-4	12.666667	1
GTGACAC	90	5.331003E-7	12.666667	19
GGCGAGA	70	1.08228705E-4	12.214286	19
TTTAGAA	55	0.0030533206	12.090909	2
GATTAAG	55	0.0030533206	12.090909	1
CCTAGAG	65	7.972981E-4	11.692307	2
CCCCCCT	65	7.972981E-4	11.692307	16
ACTGTAC	65	7.972981E-4	11.692307	3
AGCCCAG	95	1.3446765E-5	11.0	13
CCCTAGA	70	0.0014821453	10.857143	1
AGAGGTG	70	0.0014821453	10.857143	5
GCATCCC	70	0.0014821453	10.857143	12
GGCATCC	80	3.7415762E-4	10.6875	11
TGGCGAG	125	2.1994856E-7	10.639999	18
>>END_MODULE