FastQCFastQC Report
Mon 15 Aug 2016
SRR2920814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210510
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACT250.006015146619.0000024
TCTCCCC456.7280355E-414.77777910
CTCTCCC400.00525906814.259
ATAGACA500.001492049313.3000016
CAGGACT700.001482145310.8571434
TATGGCG1201.6777736E-59.516
TGGCGAG1252.7175734E-59.11999918
AGCTGCT950.00180986769.07
AGAGGTG950.00180986769.05
AATGTCC850.0073903688.9411768
ATGGCGA1401.0255854E-48.14285817
ATATGGC1906.22478E-57.015
AGGACCT1656.8178243E-46.90909055
TGGAATA1550.00268744566.741935711
CCTGGAA1709.57675E-46.70588259
AATATGG2301.10552864E-46.19565214
GAGGTGG1850.00248734236.1621626
ACCTGGA1900.00335237486.08