##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920813_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66673
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.998875106864848	33.0	32.0	34.0	30.0	34.0
2	32.14635609617086	33.0	32.0	34.0	31.0	34.0
3	32.36449537293957	33.0	32.0	34.0	32.0	34.0
4	32.44554767297107	33.0	32.0	34.0	32.0	34.0
5	32.41234082762138	33.0	32.0	34.0	32.0	34.0
6	35.31726485983832	37.0	35.0	37.0	33.0	37.0
7	35.47498987596178	37.0	35.0	37.0	33.0	37.0
8	35.36983486568776	37.0	35.0	37.0	33.0	37.0
9	35.35302146296102	37.0	35.0	37.0	33.0	37.0
10	35.415790499902506	37.0	35.0	37.0	33.0	37.0
11	35.37206965338293	37.0	34.0	37.0	33.0	37.0
12	35.34208750168734	37.0	34.0	37.0	33.0	37.0
13	36.82142696443838	38.0	38.0	38.0	36.0	38.0
14	36.97295756903094	38.0	38.0	38.0	36.0	38.0
15	36.956084172003656	38.0	38.0	38.0	36.0	38.0
16	36.8459946305101	38.0	38.0	38.0	36.0	38.0
17	36.838315360040795	38.0	38.0	38.0	36.0	38.0
18	36.90303421174988	38.0	38.0	38.0	36.0	38.0
19	36.902704243096906	38.0	38.0	38.0	36.0	38.0
20	36.84140506651868	38.0	38.0	38.0	36.0	38.0
21	36.84092511211435	38.0	38.0	38.0	36.0	38.0
22	36.893480119388656	38.0	38.0	38.0	36.0	38.0
23	37.464085911838374	39.0	38.0	39.0	36.0	39.0
24	37.30460606242407	39.0	38.0	39.0	35.0	39.0
25	37.296701813327736	39.0	38.0	39.0	35.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	6.0
17	8.0
18	18.0
19	49.0
20	57.0
21	123.0
22	149.0
23	221.0
24	244.0
25	343.0
26	386.0
27	475.0
28	573.0
29	694.0
30	851.0
31	1009.0
32	1329.0
33	1873.0
34	3156.0
35	8573.0
36	38503.0
37	8033.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	46.71006254405831	16.16246456586624	9.972552607502287	27.15492028257315
2	18.520240577145174	20.930511601397868	35.04867037631425	25.500577445142714
3	19.291167339102785	23.81473759992801	30.41111094446028	26.482984116508934
4	13.02626250506202	18.376254255845694	37.40044695753903	31.197036281553252
5	13.254240847119526	31.952964468375505	33.48881856223659	21.303976122268384
6	28.69377409146131	33.68679975402337	21.408966148215917	16.210460006299403
7	25.69855863692949	30.792074752898476	23.555262250086244	19.954104360085793
8	24.764147405996432	32.38492343227393	23.02131297526735	19.82961618646229
9	25.24110209530095	18.76621720936511	22.42586954239347	33.566811152940474
10	17.398347157020083	26.21001004904534	32.966868147525986	23.42477464640859
11	31.750483704048115	21.606947340002698	26.73646003629655	19.90610891965263
12	24.042715941985513	23.661752133547314	30.558096980786825	21.737434943680352
13	26.394492523210296	22.652348026937442	27.052929971652695	23.900229478199574
14	23.46077122673346	21.543953324434177	26.836950489703476	28.158324959128883
15	23.30328618781216	27.86285302896225	24.350186732260433	24.48367405096516
16	22.94182052704993	27.033431823976724	26.700463455971686	23.324284193001667
17	23.17129872662097	26.07502287282708	26.568475994780492	24.185202405771452
18	22.43036911493408	27.562881526255005	27.552382523660253	22.454366835150662
19	24.07871252231038	25.65956234157755	26.869947355001273	23.391777781110797
20	23.53576410241027	26.73496017878302	26.878946500082492	22.850329218724223
21	23.52376524230198	26.497982691644296	26.61347172018658	23.364780345867143
22	22.56085671861173	27.513386228308313	27.292907173818488	22.63284987926147
23	23.1682990115939	26.283503067208613	26.71696188862058	23.831236032576903
24	22.172393622605853	27.75486328798764	27.828356306150916	22.24438678325559
25	23.712747289007545	25.989530994555516	26.96743809338113	23.33028362305581
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	3.0
3	4.0
4	4.0
5	4.0
6	16.0
7	28.0
8	28.0
9	28.0
10	71.5
11	115.0
12	115.0
13	115.0
14	171.0
15	227.0
16	227.0
17	227.0
18	385.0
19	543.0
20	543.0
21	543.0
22	892.0
23	1241.0
24	1241.0
25	1241.0
26	1863.0
27	2485.0
28	2485.0
29	2485.0
30	3329.5
31	4174.0
32	4174.0
33	4174.0
34	4878.5
35	5583.0
36	5583.0
37	5583.0
38	6351.5
39	7120.0
40	7120.0
41	7120.0
42	7723.5
43	8327.0
44	8327.0
45	8327.0
46	8533.0
47	8739.0
48	8739.0
49	8739.0
50	8436.0
51	8133.0
52	8133.0
53	8133.0
54	7590.5
55	7048.0
56	7048.0
57	7048.0
58	6201.5
59	5355.0
60	5355.0
61	5355.0
62	4617.5
63	3880.0
64	3880.0
65	3880.0
66	3026.0
67	2172.0
68	2172.0
69	2172.0
70	1579.5
71	987.0
72	987.0
73	987.0
74	665.5
75	344.0
76	344.0
77	344.0
78	231.5
79	119.0
80	119.0
81	119.0
82	77.5
83	36.0
84	36.0
85	36.0
86	22.5
87	9.0
88	9.0
89	9.0
90	5.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	66673.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.68552487513686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.78281422744082	68.28551287627675
2	8.970302314055887	14.116658917402846
3	2.0205100834890013	4.7695468930451606
4	0.8329838740421638	2.621750933661302
5	0.38885288399222295	1.5298546638069384
6	0.26876596393580116	1.268879456451637
7	0.14677290229118217	0.8084231997960194
8	0.14105447752659067	0.8879156480134388
9	0.0800579467042812	0.566946140116689
>10	0.3602607601692654	4.634559716826901
>50	0.005718424764591514	0.31497007784260495
>100	0.0019061415881971712	0.19498147675970784
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	130	0.19498147675970784	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	81	0.12148845859643333	No Hit
CCCTCTCTGATTACAACATCCAGAA	74	0.11098945600167985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE