FastQCFastQC Report
Mon 15 Aug 2016
SRR2920812_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920812_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67830
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC3500.5159958720330238No Hit
GGGTCGACTCTTTCTGGATGTTGTA1910.28158631873802153No Hit
CCCTCTCTGATTACAACATCCAGAA1800.26536930561698363No Hit
GGACAAGGAGGGCATCCCCCCTGAC1360.20050125313283207No Hit
AGTCCACCCTGCACCTGGTCCTTCG1180.1739643225711337No Hit
CCTCCAGGGTGATGGTCTTGCCTGT1070.15774730945009582No Hit
CTCTCAGGCGAAGGACCAGGTGCAG970.14300457024915228No Hit
GGGTTGACTCTTTCTGGATGTTGTA960.14153029632905795No Hit
GGATAAAGAGGGCATCCCCCCTGAC950.1400560224089636No Hit
TCTCTATGGTGTCACTGGGCTCCAC920.13563320064868054No Hit
TCACAAAGATCTGCATGCCACCTCT910.13415892672858618No Hit
TCTCTATGGTGTCACTGGGCTCGAC900.13268465280849182No Hit
CCATCGAGAACGTGAAGGCCAAGAT830.12236473536783134No Hit
GCCTCAGAGGTGGCATGCAGATCTT790.11646763968745392No Hit
CTTTATCCTGGATCTTGGCCTTCAC790.11646763968745392No Hit
CCCTGGAGGTGGAGCCCAGTGACAC720.10614772224679346No Hit
GGGTTGACTCCTTCTGGATGTTGTA700.10319917440660474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCA307.619136E-419.018
GTTGACT250.00597407519.03
TGACCGG307.619136E-419.016
GACCGGC307.619136E-419.017
CTAGAGG307.619136E-419.03
CACTGGG402.712584E-416.62499813
TTCGTGA350.002141550416.2857153
CTGACCG350.002141550416.28571515
AAGACCC350.002141550416.2857159
CTTCGTG350.002141550416.2857152
TAGAGGT350.002141550416.2857154
TCTTCGT350.002141550416.2857151
CCCTAGA350.002141550416.2857151
GTCACTG508.5197884E-515.211
TGTTGTA456.6451123E-414.77777919
TCACTGG456.6451123E-414.77777912
CATCCAG400.00521052614.24999917
GAAGACC400.00521052614.2499998
TCGTGAA400.00521052614.2499994
TGAAGAC400.00521052614.2499997
Produced by FastQC (version 0.11.5)