##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920812_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67830
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900427539436826	33.0	32.0	33.0	30.0	34.0
2	31.89622585876456	33.0	32.0	34.0	30.0	34.0
3	32.18637770897833	33.0	32.0	34.0	32.0	34.0
4	32.36765443019313	33.0	32.0	34.0	32.0	34.0
5	32.20765148164529	33.0	32.0	34.0	31.0	34.0
6	34.99155241043786	37.0	34.0	37.0	32.0	37.0
7	35.33523514669025	37.0	35.0	37.0	33.0	37.0
8	35.113666519239274	37.0	34.0	37.0	32.0	37.0
9	35.29099218634823	37.0	34.0	37.0	33.0	37.0
10	35.34617425917735	37.0	34.0	37.0	33.0	37.0
11	35.30563172637476	37.0	34.0	37.0	33.0	37.0
12	35.28359133126935	37.0	34.0	37.0	33.0	37.0
13	36.898378298687895	38.0	38.0	38.0	36.0	38.0
14	37.05453339230429	38.0	38.0	38.0	37.0	38.0
15	37.03374613003096	38.0	38.0	38.0	37.0	38.0
16	36.910496830311075	38.0	38.0	38.0	36.0	38.0
17	36.902373581011354	38.0	38.0	38.0	36.0	38.0
18	36.99578357658853	38.0	38.0	38.0	36.0	38.0
19	37.00190181335692	38.0	38.0	38.0	36.0	38.0
20	36.92739200943535	38.0	38.0	38.0	36.0	38.0
21	36.93141677723721	38.0	38.0	38.0	36.0	38.0
22	36.967271118973905	38.0	38.0	38.0	36.0	38.0
23	37.560828541943096	39.0	38.0	39.0	36.0	39.0
24	37.401105705440074	39.0	38.0	39.0	36.0	39.0
25	37.37521745540322	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	9.0
19	20.0
20	28.0
21	76.0
22	86.0
23	147.0
24	241.0
25	287.0
26	395.0
27	442.0
28	588.0
29	717.0
30	864.0
31	1156.0
32	1436.0
33	2152.0
34	3630.0
35	10532.0
36	39568.0
37	5456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.17720772519534	15.243992333775616	10.501253132832082	28.077546808196963
2	20.038331121922454	19.445673006044522	33.48370927318296	27.032286598850064
3	19.859943977591037	22.24826772814389	30.185758513931887	27.706029780333186
4	13.303847854931448	16.30989237800383	36.7359575409111	33.65030222615361
5	13.505823381984372	31.670352351466903	32.22910216718266	22.594722099366063
6	30.92142120005897	33.24487689812767	19.827509951348958	16.006191950464395
7	27.126640129736106	30.106147722246796	22.434026242075777	20.333185905941324
8	27.13106295149639	30.977443609022558	21.33716644552558	20.554326993955478
9	26.209641751437417	18.084918177797434	21.245761462479727	34.45967860828542
10	17.586613592805545	25.294117647058822	32.50773993808049	24.61152882205514
11	33.60017691287041	20.2874834144184	25.872033023735806	20.240306648975377
12	25.317706029780336	23.234556980687014	29.43682736252396	22.010909627008697
13	27.13253722541648	22.170131210378887	26.280406899601942	24.416924664602682
14	24.903435058233818	20.902255639097746	24.53781512605042	29.656494176618015
15	24.110275689223055	27.36104968303111	23.20654577620522	25.322128851540615
16	23.852277753206547	25.765885301489018	26.348223499926288	24.03361344537815
17	24.745687748783723	24.207577767949285	25.034645437122215	26.012089046144776
18	23.119563614919652	26.952675807164972	26.59885006634233	23.32891051157305
19	25.76735957540911	24.176618015627305	26.031254607106003	24.024767801857585
20	25.10098776352646	25.41205956066637	25.434173669467786	24.052779006339378
21	24.148606811145513	25.204186937933066	25.630252100840334	25.016954150081084
22	22.886628335544746	26.581158779301195	26.94088161580422	23.591331269349844
23	24.593837535014003	24.474421347486363	26.165413533834585	24.766327583665046
24	23.405572755417957	27.530591183841956	26.250921421200058	22.812914639540026
25	23.96579684505381	25.58602388323751	26.22143594279817	24.226743328910512
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	10.5
7	18.0
8	18.0
9	18.0
10	54.5
11	91.0
12	91.0
13	91.0
14	129.0
15	167.0
16	167.0
17	167.0
18	259.5
19	352.0
20	352.0
21	352.0
22	653.5
23	955.0
24	955.0
25	955.0
26	1465.5
27	1976.0
28	1976.0
29	1976.0
30	2655.5
31	3335.0
32	3335.0
33	3335.0
34	3986.0
35	4637.0
36	4637.0
37	4637.0
38	5467.5
39	6298.0
40	6298.0
41	6298.0
42	7108.5
43	7919.0
44	7919.0
45	7919.0
46	8385.0
47	8851.0
48	8851.0
49	8851.0
50	8672.0
51	8493.0
52	8493.0
53	8493.0
54	8156.0
55	7819.0
56	7819.0
57	7819.0
58	7175.5
59	6532.0
60	6532.0
61	6532.0
62	5958.5
63	5385.0
64	5385.0
65	5385.0
66	4204.5
67	3024.0
68	3024.0
69	3024.0
70	2136.0
71	1248.0
72	1248.0
73	1248.0
74	858.0
75	468.0
76	468.0
77	468.0
78	322.0
79	176.0
80	176.0
81	176.0
82	118.0
83	60.0
84	60.0
85	60.0
86	37.5
87	15.0
88	15.0
89	15.0
90	11.0
91	7.0
92	7.0
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	67830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.36311366651924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.72439371718846	57.38021524399234
2	10.731365172479755	14.887218045112782
3	2.860847201853387	5.953118089340999
4	1.2688898807626092	3.520566121185316
5	0.6928946417564666	2.4030664897537966
6	0.4569703925694489	1.901813356921716
7	0.2805585666007779	1.3622291021671828
8	0.2231715870688006	1.238390092879257
9	0.11689940275032412	0.729765590446705
>10	0.5929987884970987	7.05734925549167
>50	0.03825798635465154	1.9711042311661506
>100	0.012752662118217177	1.5951643815420906
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	350	0.5159958720330238	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	191	0.28158631873802153	No Hit
CCCTCTCTGATTACAACATCCAGAA	180	0.26536930561698363	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	136	0.20050125313283207	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	118	0.1739643225711337	No Hit
CCTCCAGGGTGATGGTCTTGCCTGT	107	0.15774730945009582	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	97	0.14300457024915228	No Hit
GGGTTGACTCTTTCTGGATGTTGTA	96	0.14153029632905795	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	95	0.1400560224089636	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	92	0.13563320064868054	No Hit
TCACAAAGATCTGCATGCCACCTCT	91	0.13415892672858618	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	90	0.13268465280849182	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	83	0.12236473536783134	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	79	0.11646763968745392	No Hit
CTTTATCCTGGATCTTGGCCTTCAC	79	0.11646763968745392	No Hit
CCCTGGAGGTGGAGCCCAGTGACAC	72	0.10614772224679346	No Hit
GGGTTGACTCCTTCTGGATGTTGTA	70	0.10319917440660474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCA	30	7.619136E-4	19.0	18
GTTGACT	25	0.005974075	19.0	3
TGACCGG	30	7.619136E-4	19.0	16
GACCGGC	30	7.619136E-4	19.0	17
CTAGAGG	30	7.619136E-4	19.0	3
CACTGGG	40	2.712584E-4	16.624998	13
TTCGTGA	35	0.0021415504	16.285715	3
CTGACCG	35	0.0021415504	16.285715	15
AAGACCC	35	0.0021415504	16.285715	9
CTTCGTG	35	0.0021415504	16.285715	2
TAGAGGT	35	0.0021415504	16.285715	4
TCTTCGT	35	0.0021415504	16.285715	1
CCCTAGA	35	0.0021415504	16.285715	1
GTCACTG	50	8.5197884E-5	15.2	11
TGTTGTA	45	6.6451123E-4	14.777779	19
TCACTGG	45	6.6451123E-4	14.777779	12
CATCCAG	40	0.005210526	14.249999	17
GAAGACC	40	0.005210526	14.249999	8
TCGTGAA	40	0.005210526	14.249999	4
TGAAGAC	40	0.005210526	14.249999	7
>>END_MODULE