FastQCFastQC Report
Mon 15 Aug 2016
SRR2920812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67830
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC3680.5425328025947221No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2080.30664897537962554No Hit
CCCTCTCTGATTACAACATCCAGAA1630.24030664897537962No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1520.22408963585434172No Hit
AGTCCACCCTGCACCTGGTCCTTCG1430.21082117057349253No Hit
CTCTCAGGCGAAGGACCAGGTGCAG1340.19755270529264335No Hit
GGGTCGACTCTTTCTGGATGTTGTA1330.19607843137254902No Hit
TCTCTATGGTGTCACTGGGCTCCAC1230.18133569217160547No Hit
GGATAAAGAGGGCATCCCCCCTGAC1090.16069585729028452No Hit
GGACAAGGAGGGCATCCCCCCTGAC1080.15922158337019018No Hit
GCCTCAGAGGTGGCATGCAGATCTT1060.15627303553000146No Hit
CCTCCAGGGTGATGGTCTTGCCTGT960.14153029632905795No Hit
TCTCTATGGTGTCACTGGGCTCGAC900.13268465280849182No Hit
TCACAAAGATCTGCATGCCACCTCT870.12826183104820876No Hit
GGGTTGACTCCTTCTGGATGTTGTA850.12531328320802004No Hit
TGCCTGCAAAGATCAGCCTCTGCTG770.11351909184726522No Hit
CCATCGAGAACGTGAAGGCCAAGAT750.11057054400707653No Hit
GGGTTGACTCTTTCTGGATGTTGTA720.10614772224679346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTA552.7217538E-819.019
GATGTTG552.7217538E-819.017
CCTAGAG502.097986E-719.02
AGGTGGA607.3698175E-817.4166687
CTGGATG607.3698175E-817.41666814
CTAGAGG607.3698175E-817.4166683
TAGAGGT555.7070065E-717.2727284
CCCTAGA555.7070065E-717.2727281
GTTGACT504.4339577E-617.0999983
GGTTGAC504.4339577E-617.0999982
TTGACTC504.4339577E-617.0999984
AAGGAGG350.002141550416.2857155
AGCCCAG651.8355058E-716.07692313
TCTGGAT651.8355058E-716.07692313
TTCTGGA651.8355058E-716.07692312
AGTGACA651.8355058E-716.07692318
GCCCAGT704.257272E-714.92857214
GAGCCCA704.257272E-714.92857212
TGGATGT704.257272E-714.92857215
ATGTTGT704.257272E-714.92857218
Produced by FastQC (version 0.11.5)