##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920812_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67830
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6594132389798	33.0	32.0	34.0	32.0	34.0
2	32.92444346159517	33.0	32.0	34.0	32.0	34.0
3	33.12914639540026	33.0	33.0	34.0	32.0	34.0
4	33.209818664307825	33.0	33.0	34.0	32.0	34.0
5	32.97075040542533	33.0	33.0	34.0	32.0	34.0
6	35.774907857879995	37.0	35.0	37.0	33.0	37.0
7	35.85931003980539	37.0	37.0	37.0	33.0	37.0
8	35.81076219961669	37.0	35.0	37.0	33.0	37.0
9	35.73409995577178	37.0	35.0	37.0	33.0	37.0
10	35.70392156862745	37.0	35.0	37.0	33.0	37.0
11	35.69128704113224	37.0	34.0	37.0	33.0	37.0
12	35.59181777974348	37.0	34.0	37.0	33.0	37.0
13	37.270440807902105	38.0	38.0	38.0	37.0	38.0
14	37.410216718266255	38.0	38.0	38.0	37.0	38.0
15	37.45120153324488	38.0	38.0	38.0	37.0	38.0
16	37.420993660622145	38.0	38.0	38.0	37.0	38.0
17	37.41388766032729	38.0	38.0	38.0	37.0	38.0
18	37.39352793749079	38.0	38.0	38.0	37.0	38.0
19	37.386392451717526	38.0	38.0	38.0	37.0	38.0
20	37.382736252395695	38.0	38.0	38.0	37.0	38.0
21	37.3843284682294	38.0	38.0	38.0	37.0	38.0
22	37.41509656494176	38.0	38.0	38.0	37.0	38.0
23	38.05845496093174	39.0	38.0	39.0	37.0	39.0
24	38.04605631726375	39.0	38.0	39.0	37.0	39.0
25	37.99523809523809	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	4.0
24	10.0
25	33.0
26	54.0
27	125.0
28	213.0
29	370.0
30	503.0
31	770.0
32	1094.0
33	1650.0
34	2894.0
35	8108.0
36	41854.0
37	10148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.218929677134014	16.181630546955624	10.710600029485478	27.888839746424885
2	19.737579242223205	19.607843137254903	33.46601798614183	27.188559634380066
3	20.06781660032434	21.839893852277754	30.249152292495946	27.84313725490196
4	13.510246203744655	16.081379920389207	36.79050567595459	33.61786819991154
5	13.234556980687012	32.49742002063984	31.400560224089634	22.867462774583515
6	31.24871001031992	33.37166445525578	19.492849771487542	15.886775762936752
7	27.082411912133274	30.554326993955478	22.293970219666814	20.069290874244437
8	27.87409700722394	31.02314610054548	21.25902992776058	19.843726964469997
9	25.882352941176475	18.015627303553	21.527347781217752	34.57467197405278
10	17.285861713106296	25.590446704997788	32.73182957393484	24.39186200796108
11	33.60017691287041	20.084033613445378	25.898569954297507	20.417219519386702
12	25.481350434910805	22.9500221141088	29.51643815420905	22.052189296771342
13	27.231313578062803	22.227627893262568	26.417514374170718	24.123544154503907
14	24.58499189149344	20.40542532802595	25.098039215686274	29.91154356479434
15	23.805100987763527	27.159074155978182	23.547103051747015	25.48872180451128
16	23.737284387439185	25.805690697331563	26.632758366504493	23.824266548724754
17	24.81940144478844	23.970219666814092	25.438596491228072	25.771782397169396
18	23.446852425180598	26.89075630252101	26.65339820138582	23.008993070912574
19	25.749668288367978	24.235588972431078	26.352646321686567	23.662096417514373
20	25.15553589856995	25.31475748194014	26.00324340262421	23.526463216865693
21	24.284240011794193	24.85330974495061	26.283355447442137	24.579094795813063
22	23.26699100692909	26.208167477517325	27.094206103494027	23.430635412059562
23	24.47589562140646	24.27834291611381	26.725637623470437	24.52012383900929
24	23.191803037004274	26.983635559486952	27.111897390535162	22.71266401297361
25	24.262125902992775	24.938817632316084	26.659295297066194	24.139761167624947
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	10.0
7	18.0
8	18.0
9	18.0
10	117.5
11	217.0
12	217.0
13	217.0
14	183.0
15	149.0
16	149.0
17	149.0
18	249.5
19	350.0
20	350.0
21	350.0
22	744.5
23	1139.0
24	1139.0
25	1139.0
26	1540.5
27	1942.0
28	1942.0
29	1942.0
30	2613.0
31	3284.0
32	3284.0
33	3284.0
34	3972.0
35	4660.0
36	4660.0
37	4660.0
38	5489.5
39	6319.0
40	6319.0
41	6319.0
42	7115.5
43	7912.0
44	7912.0
45	7912.0
46	8274.0
47	8636.0
48	8636.0
49	8636.0
50	8572.0
51	8508.0
52	8508.0
53	8508.0
54	8179.5
55	7851.0
56	7851.0
57	7851.0
58	7220.5
59	6590.0
60	6590.0
61	6590.0
62	5974.0
63	5358.0
64	5358.0
65	5358.0
66	4176.5
67	2995.0
68	2995.0
69	2995.0
70	2109.5
71	1224.0
72	1224.0
73	1224.0
74	840.5
75	457.0
76	457.0
77	457.0
78	300.5
79	144.0
80	144.0
81	144.0
82	100.5
83	57.0
84	57.0
85	57.0
86	34.5
87	12.0
88	12.0
89	12.0
90	8.5
91	5.0
92	5.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	67830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.60975969335102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69801022820062	56.73890608875129
2	10.623576432162963	14.577620521892968
3	2.838540547509562	5.8425475453339235
4	1.2957153294082255	3.5559486952675807
5	0.767115045769049	2.631578947368421
6	0.49207099574541235	2.0256523662096417
7	0.27504405002363663	1.3209494324045408
8	0.20198547423610813	1.1086539879109538
9	0.15471227813829558	0.9553295002211412
>10	0.595212514504276	7.052926433731388
>50	0.03438050625295458	1.614329942503317
>100	0.02363659804890627	2.5755565384048356
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	368	0.5425328025947221	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	208	0.30664897537962554	No Hit
CCCTCTCTGATTACAACATCCAGAA	163	0.24030664897537962	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	152	0.22408963585434172	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	143	0.21082117057349253	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	134	0.19755270529264335	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	133	0.19607843137254902	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	123	0.18133569217160547	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	109	0.16069585729028452	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	108	0.15922158337019018	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	106	0.15627303553000146	No Hit
CCTCCAGGGTGATGGTCTTGCCTGT	96	0.14153029632905795	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	90	0.13268465280849182	No Hit
TCACAAAGATCTGCATGCCACCTCT	87	0.12826183104820876	No Hit
GGGTTGACTCCTTCTGGATGTTGTA	85	0.12531328320802004	No Hit
TGCCTGCAAAGATCAGCCTCTGCTG	77	0.11351909184726522	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	75	0.11057054400707653	No Hit
GGGTTGACTCTTTCTGGATGTTGTA	72	0.10614772224679346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGTA	55	2.7217538E-8	19.0	19
GATGTTG	55	2.7217538E-8	19.0	17
CCTAGAG	50	2.097986E-7	19.0	2
AGGTGGA	60	7.3698175E-8	17.416668	7
CTGGATG	60	7.3698175E-8	17.416668	14
CTAGAGG	60	7.3698175E-8	17.416668	3
TAGAGGT	55	5.7070065E-7	17.272728	4
CCCTAGA	55	5.7070065E-7	17.272728	1
GTTGACT	50	4.4339577E-6	17.099998	3
GGTTGAC	50	4.4339577E-6	17.099998	2
TTGACTC	50	4.4339577E-6	17.099998	4
AAGGAGG	35	0.0021415504	16.285715	5
AGCCCAG	65	1.8355058E-7	16.076923	13
TCTGGAT	65	1.8355058E-7	16.076923	13
TTCTGGA	65	1.8355058E-7	16.076923	12
AGTGACA	65	1.8355058E-7	16.076923	18
GCCCAGT	70	4.257272E-7	14.928572	14
GAGCCCA	70	4.257272E-7	14.928572	12
TGGATGT	70	4.257272E-7	14.928572	15
ATGTTGT	70	4.257272E-7	14.928572	18
>>END_MODULE