FastQCFastQC Report
Mon 15 Aug 2016
SRR2920811_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920811_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171758
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC11010.641018176737037No Hit
CCCTCTCTGATTACAACATCCAGAA5530.32196462464630465No Hit
GGGTCGACTCTTTCTGGATGTTGTA5390.313813621490702No Hit
AGTCCACCCTGCACCTGGTCCTTCG4410.2567565994014835No Hit
GGACAAGGAGGGCATCCCCCCTGAC4060.23637909151247688No Hit
CTCTCAGGCGAAGGACCAGGTGCAG3930.22881030286798867No Hit
GGATAAAGAGGGCATCCCCCCTGAC3190.18572642904551753No Hit
TCTCTATGGTGTCACTGGGCTCCAC3160.1839797855121741No Hit
TCACAAAGATCTGCATGCCACCTCT3000.1746643533343425No Hit
GCCTCAGAGGTGGCATGCAGATCTT2990.17408213882322804No Hit
GGGTTGACTCCTTCTGGATGTTGTA2960.1723354952898846No Hit
CCATCGAGAACGTGAAGGCCAAGAT2860.16651335017873986No Hit
TCTCTATGGTGTCACTGGGCTCGAC2830.16476670664539644No Hit
CCTCCAGGGTGATGGTCTTGCCTGT2590.15079355837864902No Hit
CTTTATCCTGGATCTTGGCCTTCAC2550.14846470033419112No Hit
CTGTAGGACCTGGAATATGGCGAGA2530.1473002713119622No Hit
GGGTTGACTCTTTCTGGATGTTGTA2420.14089591168970295No Hit
GTCCTTCAGTGTGCATTTCTCATTT2240.13041605048964242No Hit
GAGTCCACCCTGCACCTGGTCCTTC2010.11702511673400949No Hit
CCCTGGAGGTGGAGCCCAGTGACAC2000.11644290222289501No Hit
GGCGAAGGACCAGGTGCAGGGTTGA1970.11469625868955158No Hit
CCTCCAGGGTGATGGTCTTGCCAGT1930.11236740064509368No Hit
CTGAAGGACCTGGAATATGGCGAGA1850.10770968455617788No Hit
GCTCCACCTCCAGGGTGATGGTCTT1780.10363418297837654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTG307.684573E-419.05
CATTAGC250.00601073619.03
CCCTAGA1200.016.6251
TGTTGTA1200.016.62519
ATTAGCT350.00215964816.2857154
GGGCTCG601.4483594E-615.83333417
GTCGACT551.1166978E-515.5454553
GGTCGAC551.1166978E-515.5454552
CCTAGAG1300.015.3461532
CTAGAGG1250.015.23
TAGAGGT1250.015.24
GGCTCGA653.330133E-614.61538418
TATGGTG1058.54925E-1114.47619155
ATGGTGT1450.014.4137936
CGACTCT602.542127E-514.2500015
GCAAGCA400.005253847714.257
TCACTGG1400.014.2512
TGGGCTC1400.014.2516
CTATGGT1152.3646862E-1114.0434784
TCTCTAT1152.3646862E-1114.0434781
Produced by FastQC (version 0.11.5)