FastQCFastQC Report
Mon 15 Aug 2016
SRR2920811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171758
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC10930.6363604606481211No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5710.3324444858463652No Hit
CCCTCTCTGATTACAACATCCAGAA5470.3184713375796179No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4790.27888075082383357No Hit
CTCTCAGGCGAAGGACCAGGTGCAG4760.2771341072904901No Hit
GGGTCGACTCTTTCTGGATGTTGTA4640.2701475331571164No Hit
AGTCCACCCTGCACCTGGTCCTTCG4260.24802338173476635No Hit
GCCTCAGAGGTGGCATGCAGATCTT3690.21483715460124128No Hit
TCTCTATGGTGTCACTGGGCTCCAC3620.21076165302343994No Hit
GGACAAGGAGGGCATCCCCCCTGAC3520.2049395079122952No Hit
GGATAAAGAGGGCATCCCCCCTGAC3320.1932952176900057No Hit
TCACAAAGATCTGCATGCCACCTCT3200.18630864355663201No Hit
TCTCTATGGTGTCACTGGGCTCGAC2960.1723354952898846No Hit
GGGTTGACTCCTTCTGGATGTTGTA2680.1560334889786793No Hit
GGGTTGACTCTTTCTGGATGTTGTA2560.1490469148453056No Hit
CCATCGAGAACGTGAAGGCCAAGAT2540.14788248582307664No Hit
CTGTAGGACCTGGAATATGGCGAGA2400.139731482667474No Hit
CCTCCAGGGTGATGGTCTTGCCTGT2370.1379848391341306No Hit
CTTTATCCTGGATCTTGGCCTTCAC2280.13274490853410031No Hit
GCTCCACCTCCAGGGTGATGGTCTT2130.12401169086738317No Hit
TGCCTGCAAAGATCAGCCTCTGCTG2070.12051840380069632No Hit
GTCCTTCAGTGTGCATTTCTCATTT2070.12051840380069632No Hit
GGCGAAGGACCAGGTGCAGGGTTGA1970.11469625868955158No Hit
CCCTGGAGGTGGAGCCCAGTGACAC1950.11353182966732263No Hit
CCTCCAGGGTGATGGTCTTGCCAGT1880.10945632808952131No Hit
CTGAAGGACCTGGAATATGGCGAGA1860.10829189906729235No Hit
GAGTCCACCCTGCACCTGGTCCTTC1830.10654525553394892No Hit
CCTCTCAGGCGAAGGACCAGGTGCA1740.10130532493391865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAAT250.00601073619.08
GAGAACG250.00601073619.06
AGAGTAC802.1827873E-1117.81255
ACGCAGA751.6734703E-1017.7333341
CGCAGAG751.6734703E-1017.7333342
TAGAGGT1100.017.2727284
CCCTAGA1250.016.721
CTAGAGG1200.016.6253
TCGAGAA402.7432197E-416.6254
GGCTCGA402.7432197E-416.62518
ATCGAGA402.7432197E-416.6253
ACTCCTA350.00215964816.2857156
CCTAGAG1250.015.962
TCACAAA508.642249E-515.21
CCATCGA508.642249E-515.21
CAGAGTA952.9649527E-1015.04
GGGCTCG456.719109E-414.77777817
CATCGAG456.719109E-414.7777782
TGGGCTC1058.54925E-1114.476191516
TCCTATT400.005253847714.258
Produced by FastQC (version 0.11.5)