FastQCFastQC Report
Mon 15 Aug 2016
SRR2920810_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920810_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124547
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC6950.558022272716324No Hit
GGGTCGACTCTTTCTGGATGTTGTA4140.33240463439504764No Hit
CCCTCTCTGATTACAACATCCAGAA3670.2946678763840157No Hit
AGTCCACCCTGCACCTGGTCCTTCG2560.20554489469838694No Hit
GGACAAGGAGGGCATCCCCCCTGAC2460.19751579724923124No Hit
GGATAAAGAGGGCATCCCCCCTGAC2430.1951070680144845No Hit
CTCTCAGGCGAAGGACCAGGTGCAG2350.1886837900551599No Hit
CCTCCAGGGTGATGGTCTTGCCTGT2120.1702168659221017No Hit
TCACAAAGATCTGCATGCCACCTCT2080.1670052269424394No Hit
TCTCTATGGTGTCACTGGGCTCCAC2050.16459649770769266No Hit
GCCTCAGAGGTGGCATGCAGATCTT1970.1581732197483681No Hit
TCTCTATGGTGTCACTGGGCTCGAC1910.15335576127887465No Hit
CTGTAGGACCTGGAATATGGCGAGA1790.14372084433988774No Hit
CCATCGAGAACGTGAAGGCCAAGAT1550.12445101046191398No Hit
GGGTTGACTCCTTCTGGATGTTGTA1530.12284519097208284No Hit
GGGTTGACTCTTTCTGGATGTTGTA1520.12204228122716726No Hit
GTCCTTCAGTGTGCATTTCTCATTT1460.11722482275767382No Hit
CCCTGGAGGTGGAGCCCAGTGACAC1440.11561900326784266No Hit
GAGTCCACCCTGCACCTGGTCCTTC1410.11321027403309594No Hit
CTTTATCCTGGATCTTGGCCTTCAC1360.10919572530851807No Hit
CTGAAGGACCTGGAATATGGCGAGA1300.10437826683902462No Hit
TGCCTGCAAAGATCAGCCTCTGCTG1250.10036371811444676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCTG250.00600164719.05
TATAGCT250.00600164719.04
ATCGAGA402.7356116E-416.6253
TCAGGCG350.00215515816.2857154
CAGGCGA350.00215515816.2857155
GAGCCCA1400.016.28571512
TCGAGAA350.00215515816.2857154
CTAGAGG1050.016.2857133
TAGAGGT1050.016.2857134
TGGAGCC1300.016.07692310
GTCGACT601.4405468E-615.8333323
GGTCGAC601.4405468E-615.8333322
GGGTCGA601.4405468E-615.8333321
GGAGCCC1350.015.48148111
CCTAGAG1250.015.22
CCCTAGA1200.015.0416661
GGTGGAG1400.014.9285728
GTACATG456.7007344E-414.7777781
CCATCGA456.7007344E-414.7777781
GATATAC456.7007344E-414.7777781
Produced by FastQC (version 0.11.5)