##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920810_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124547
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960167647554737	33.0	32.0	33.0	30.0	34.0
2	32.27359952467743	33.0	32.0	34.0	32.0	34.0
3	32.48902020923828	33.0	32.0	34.0	32.0	34.0
4	32.542799103952724	33.0	32.0	34.0	32.0	34.0
5	32.43252747958602	33.0	32.0	34.0	32.0	34.0
6	35.44520542445824	37.0	34.0	37.0	33.0	37.0
7	35.403269448481296	37.0	35.0	37.0	33.0	37.0
8	35.44476382409853	37.0	35.0	37.0	33.0	37.0
9	35.45368415136455	37.0	34.0	37.0	33.0	37.0
10	35.45162870241756	37.0	34.0	37.0	33.0	37.0
11	35.47051313961798	37.0	34.0	37.0	33.0	37.0
12	35.431451580527835	37.0	34.0	37.0	33.0	37.0
13	37.029065332765946	38.0	38.0	38.0	37.0	38.0
14	37.15826956891776	38.0	38.0	38.0	37.0	38.0
15	37.14969449284206	38.0	38.0	38.0	37.0	38.0
16	37.04870450512658	38.0	38.0	38.0	37.0	38.0
17	37.03898126811565	38.0	38.0	38.0	37.0	38.0
18	37.10074911479201	38.0	38.0	38.0	37.0	38.0
19	37.10706801448449	38.0	38.0	38.0	37.0	38.0
20	37.047676780653084	38.0	38.0	38.0	37.0	38.0
21	37.0694677511301	38.0	38.0	38.0	37.0	38.0
22	37.11392486370607	38.0	38.0	38.0	37.0	38.0
23	37.68062659076493	39.0	38.0	39.0	37.0	39.0
24	37.54845961765438	39.0	38.0	39.0	36.0	39.0
25	37.50413097063759	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	5.0
17	14.0
18	22.0
19	25.0
20	51.0
21	91.0
22	187.0
23	241.0
24	351.0
25	469.0
26	626.0
27	663.0
28	856.0
29	1143.0
30	1345.0
31	1748.0
32	2298.0
33	3197.0
34	6082.0
35	16993.0
36	76198.0
37	11940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.00951448047725	16.05096871060724	11.050446819273045	27.88906998964246
2	19.364577227873813	20.128144395288526	34.185488209270396	26.321790167567265
3	19.465743855733177	22.9158470296354	30.985892875781833	26.63251623884959
4	12.861008294057665	16.975920736749984	37.26464708102162	32.89842388817073
5	12.297365653126931	32.06982103141785	31.478879459159998	24.153933856295215
6	29.37525592748119	34.04497900391017	20.72952379423029	15.850241274378346
7	26.139529655471428	31.340778983034518	22.526435803351344	19.993255558142707
8	27.132729009931992	31.399391394413357	21.546885914554345	19.920993681100306
9	26.076902695368016	19.129324672613553	21.67454856399592	33.11922406802251
10	17.399857082065406	25.18727869800156	32.977911952917374	24.434952267015664
11	33.02769235710214	19.75720009313753	27.50527913157282	19.70982841818751
12	25.440195267649962	23.288397151276225	29.902767629890725	21.368639951183088
13	26.42215388568171	22.886942278818438	26.89908227416156	23.79182156133829
14	24.66779609304118	21.565352838687403	24.605972042682684	29.16087902558873
15	23.509197331128007	28.123519635157813	23.416862710462716	24.950420323251464
16	23.16234032132448	26.074493966133268	27.42900270580584	23.334163006736414
17	24.390792231045307	24.944799955037052	25.171220503103246	25.493187310814392
18	22.918255758870146	27.234698547536272	27.184115233606594	22.662930459986992
19	25.30530643050415	24.47429484451653	26.37478221073169	23.845616514247634
20	24.7408608798285	26.098581258480735	25.970918609039156	23.18963925265161
21	23.798244839297613	25.589536480204263	25.956466233630675	24.65575244686745
22	22.617164604526803	27.102218439625204	27.297325507639687	22.983291448208305
23	24.33217981966647	25.067645146009138	26.430985892875782	24.169189141448612
24	22.683003203609882	27.755786971986478	27.26761784707781	22.29359197732583
25	23.612772688222115	25.860117064240807	26.503247769918183	24.02386247761889
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	2.5
3	4.0
4	4.0
5	4.0
6	10.0
7	16.0
8	16.0
9	16.0
10	61.5
11	107.0
12	107.0
13	107.0
14	179.0
15	251.0
16	251.0
17	251.0
18	462.0
19	673.0
20	673.0
21	673.0
22	1280.5
23	1888.0
24	1888.0
25	1888.0
26	3003.0
27	4118.0
28	4118.0
29	4118.0
30	5696.5
31	7275.0
32	7275.0
33	7275.0
34	8543.0
35	9811.0
36	9811.0
37	9811.0
38	11288.0
39	12765.0
40	12765.0
41	12765.0
42	13892.0
43	15019.0
44	15019.0
45	15019.0
46	15594.5
47	16170.0
48	16170.0
49	16170.0
50	15614.5
51	15059.0
52	15059.0
53	15059.0
54	14239.5
55	13420.0
56	13420.0
57	13420.0
58	12268.0
59	11116.0
60	11116.0
61	11116.0
62	10172.5
63	9229.0
64	9229.0
65	9229.0
66	6990.0
67	4751.0
68	4751.0
69	4751.0
70	3323.0
71	1895.0
72	1895.0
73	1895.0
74	1270.0
75	645.0
76	645.0
77	645.0
78	449.5
79	254.0
80	254.0
81	254.0
82	154.5
83	55.0
84	55.0
85	55.0
86	36.5
87	18.0
88	18.0
89	18.0
90	12.0
91	6.0
92	6.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	124547.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.88261459529335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15448767799201	47.399776791090915
2	11.89831326593548	14.250042152761608
3	3.590678716044086	6.450576890651721
4	1.720254216835161	4.1205328109067265
5	0.930519428280282	2.786096814857042
6	0.6248156391622643	2.244935646783945
7	0.3821297363975222	1.6018049411065702
8	0.2989997586549033	1.432390984929384
9	0.21855139309753024	1.1778685957911472
>10	1.0672816497278164	10.999863505343365
>50	0.07776675337212732	3.1329538246605697
>100	0.034860958408195	3.8451347684006842
>500	0.001340806092622885	0.558022272716324
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	695	0.558022272716324	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	414	0.33240463439504764	No Hit
CCCTCTCTGATTACAACATCCAGAA	367	0.2946678763840157	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	256	0.20554489469838694	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	246	0.19751579724923124	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	243	0.1951070680144845	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	235	0.1886837900551599	No Hit
CCTCCAGGGTGATGGTCTTGCCTGT	212	0.1702168659221017	No Hit
TCACAAAGATCTGCATGCCACCTCT	208	0.1670052269424394	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	205	0.16459649770769266	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	197	0.1581732197483681	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	191	0.15335576127887465	No Hit
CTGTAGGACCTGGAATATGGCGAGA	179	0.14372084433988774	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	155	0.12445101046191398	No Hit
GGGTTGACTCCTTCTGGATGTTGTA	153	0.12284519097208284	No Hit
GGGTTGACTCTTTCTGGATGTTGTA	152	0.12204228122716726	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	146	0.11722482275767382	No Hit
CCCTGGAGGTGGAGCCCAGTGACAC	144	0.11561900326784266	No Hit
GAGTCCACCCTGCACCTGGTCCTTC	141	0.11321027403309594	No Hit
CTTTATCCTGGATCTTGGCCTTCAC	136	0.10919572530851807	No Hit
CTGAAGGACCTGGAATATGGCGAGA	130	0.10437826683902462	No Hit
TGCCTGCAAAGATCAGCCTCTGCTG	125	0.10036371811444676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCTG	25	0.006001647	19.0	5
TATAGCT	25	0.006001647	19.0	4
ATCGAGA	40	2.7356116E-4	16.625	3
TCAGGCG	35	0.002155158	16.285715	4
CAGGCGA	35	0.002155158	16.285715	5
GAGCCCA	140	0.0	16.285715	12
TCGAGAA	35	0.002155158	16.285715	4
CTAGAGG	105	0.0	16.285713	3
TAGAGGT	105	0.0	16.285713	4
TGGAGCC	130	0.0	16.076923	10
GTCGACT	60	1.4405468E-6	15.833332	3
GGTCGAC	60	1.4405468E-6	15.833332	2
GGGTCGA	60	1.4405468E-6	15.833332	1
GGAGCCC	135	0.0	15.481481	11
CCTAGAG	125	0.0	15.2	2
CCCTAGA	120	0.0	15.041666	1
GGTGGAG	140	0.0	14.928572	8
GTACATG	45	6.7007344E-4	14.777778	1
CCATCGA	45	6.7007344E-4	14.777778	1
GATATAC	45	6.7007344E-4	14.777778	1
>>END_MODULE