FastQCFastQC Report
Mon 15 Aug 2016
SRR2920810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124547
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC6890.5532048142468304No Hit
CCCTCTCTGATTACAACATCCAGAA3860.30992316153741156No Hit
GGGTCGACTCTTTCTGGATGTTGTA3320.26656603531197054No Hit
AGTCCACCCTGCACCTGGTCCTTCG3030.24328165270941896No Hit
CTCTCAGGCGAAGGACCAGGTGCAG2910.23364673577043207No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2830.22722345781110742No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2460.19751579724923124No Hit
GGATAAAGAGGGCATCCCCCCTGAC2390.19189542903482218No Hit
GGACAAGGAGGGCATCCCCCCTGAC2360.1894866998000755No Hit
TCTCTATGGTGTCACTGGGCTCCAC2340.18788088031024433No Hit
TCTCTATGGTGTCACTGGGCTCGAC2150.17262559515684842No Hit
CCTCCAGGGTGATGGTCTTGCCTGT2100.16861104643227054No Hit
GCCTCAGAGGTGGCATGCAGATCTT2020.16218776847294597No Hit
TCACAAAGATCTGCATGCCACCTCT1940.15576449051362135No Hit
CCATCGAGAACGTGAAGGCCAAGAT1690.13569174689073202No Hit
CTTTATCCTGGATCTTGGCCTTCAC1670.13408592740090086No Hit
CTGTAGGACCTGGAATATGGCGAGA1620.13007137867632299No Hit
GGGTTGACTCCTTCTGGATGTTGTA1610.12926846893140742No Hit
GTCCTTCAGTGTGCATTTCTCATTT1450.11642191301275823No Hit
CCCTGGAGGTGGAGCCCAGTGACAC1440.11561900326784266No Hit
GGGTTGACTCTTTCTGGATGTTGTA1300.10437826683902462No Hit
GCTCCACCTCCAGGGTGATGGTCTT1290.10357535709410906No Hit
GAGTCCACCCTGCACCTGGTCCTTC1280.10277244734919347No Hit
CCTCTCAGGCGAAGGACCAGGTGCA1280.10277244734919347No Hit
CTGAAGGACCTGGAATATGGCGAGA1270.1019695376042779No Hit
TGCCTGCAAAGATCAGCCTCTGCTG1250.10036371811444676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACGT250.00600164719.07
AGAGTAC359.846531E-519.05
GAGAACG250.00600164719.06
CCCCCCT401.2697685E-519.016
GAACGTG250.00600164719.08
TCCCCCC401.2697685E-519.015
ACGCAGA307.668337E-418.9999981
CGGCAAA307.668337E-418.99999817
TAGAGGT555.802758E-717.2727284
GTCGACT453.4968907E-516.888893
TCGACTC453.4968907E-516.888894
TCACAAA350.00215515816.2857151
CTAGAGG601.4405468E-615.8333323
CCTAGAG551.1117741E-515.5454552
TCTGCAT508.6118074E-515.210
GGTCGAC508.6118074E-515.22
ATCCCCC508.6118074E-515.214
CAGAGTA508.6118074E-515.24
CCATCGA456.7007344E-414.7777781
TAGGACC456.7007344E-414.7777784
Produced by FastQC (version 0.11.5)