FastQCFastQC Report
Mon 15 Aug 2016
SRR2920809_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920809_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123739
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC4810.3887214217021311No Hit
CCCTCTCTGATTACAACATCCAGAA3100.2505273196001261No Hit
GGGTCGACTCTTTCTGGATGTTGTA3080.24891101431238333No Hit
GGATAAAGAGGGCATCCCCCCTGAC2150.1737528184323455No Hit
AGTCCACCCTGCACCTGGTCCTTCG1820.14708378118459015No Hit
GGACAAGGAGGGCATCCCCCCTGAC1770.14304301796523328No Hit
CTCTCAGGCGAAGGACCAGGTGCAG1640.1325370335949054No Hit
GCCTCAGAGGTGGCATGCAGATCTT1430.11556582807360655No Hit
TCTCTATGGTGTCACTGGGCTCCAC1410.11394952278586379No Hit
CCATCGAGAACGTGAAGGCCAAGAT1370.11071691221037828No Hit
CTTTATCCTGGATCTTGGCCTTCAC1340.10829245427876417No Hit
TCTCTATGGTGTCACTGGGCTCGAC1300.10505984370327867No Hit
TCACAAAGATCTGCATGCCACCTCT1280.10344353841553593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGGT502.1332744E-719.04
CCTAGAG502.1332744E-719.02
CTAGAGG555.8020123E-717.2727283
AGGGTGA453.496596E-516.888896
CCCCCCT651.8719038E-716.07692316
TCCCCCC651.8719038E-716.07692315
GATAAAG508.611091E-515.22
CCCTAGA704.3409273E-714.9285721
TGCCAGT456.7003013E-414.77777819
AGAGGTG1101.0913936E-1114.6818185
AAAGAGG602.5307821E-514.2500015
GTTGACT551.9373038E-413.8181823
GGGTGAT551.9373038E-413.8181827
AAAGTGT551.9373038E-413.8181826
GGTTGAC551.9373038E-413.8181822
GATGTTG1051.3187673E-913.57142917
GGAGCCC1009.735231E-913.29999911
GGCGAGA500.001485985913.29999919
AGTGACA1153.619789E-1013.21739118
GTGACAC1153.619789E-1013.21739119
Produced by FastQC (version 0.11.5)