##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920809_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123739
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.419657504909527	32.0	32.0	33.0	29.0	34.0
2	31.99737350390742	33.0	32.0	33.0	30.0	34.0
3	32.279224819984	33.0	32.0	34.0	32.0	34.0
4	32.34942095863067	33.0	32.0	34.0	32.0	34.0
5	32.150712386555576	33.0	32.0	34.0	31.0	34.0
6	35.24701993712572	37.0	34.0	37.0	32.0	37.0
7	35.357494403542944	37.0	35.0	37.0	33.0	37.0
8	35.203104922457754	37.0	34.0	37.0	33.0	37.0
9	35.313684448718675	37.0	34.0	37.0	33.0	37.0
10	35.357914642917756	37.0	34.0	37.0	33.0	37.0
11	35.31199540969298	37.0	34.0	37.0	33.0	37.0
12	35.30394620936002	37.0	34.0	37.0	33.0	37.0
13	36.88563831936576	38.0	38.0	38.0	36.0	38.0
14	37.02594978139471	38.0	38.0	38.0	37.0	38.0
15	37.01214653423739	38.0	38.0	38.0	37.0	38.0
16	36.90092048586137	38.0	38.0	38.0	36.0	38.0
17	36.894738118135756	38.0	38.0	38.0	36.0	38.0
18	36.96786785087967	38.0	38.0	38.0	36.0	38.0
19	36.985210806617154	38.0	38.0	38.0	36.0	38.0
20	36.936309490136495	38.0	38.0	38.0	36.0	38.0
21	36.93699641988379	38.0	38.0	38.0	36.0	38.0
22	36.984830974874534	38.0	38.0	38.0	36.0	38.0
23	37.5314169340305	39.0	38.0	39.0	36.0	39.0
24	37.391751994116646	39.0	38.0	39.0	36.0	39.0
25	37.36876005139851	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	7.0
18	10.0
19	41.0
20	80.0
21	128.0
22	231.0
23	328.0
24	429.0
25	513.0
26	725.0
27	922.0
28	1073.0
29	1326.0
30	1575.0
31	2001.0
32	2417.0
33	3644.0
34	6306.0
35	18804.0
36	74998.0
37	8179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.54685264952844	15.184380025699253	10.067965637349582	27.20080168742272
2	20.354132488544437	19.569416271345332	33.22881225805931	26.84763898205093
3	19.818327285657716	22.604837601726214	30.32754426656107	27.249290846055
4	13.102578815086593	16.515407430155406	37.47727070689112	32.90474304786688
5	13.490492084144853	31.741003240692102	32.36812969233629	22.400374982826758
6	30.130355021456452	33.39448355005293	20.323422688077326	16.15173874041329
7	26.891279224819986	30.30653229782041	22.488463621008737	20.313724856350866
8	26.656914958097282	31.130039841925345	21.700514793234145	20.512530406743227
9	26.061306459564083	18.626302135947437	21.533227195952772	33.779164208535704
10	17.56358140925658	25.419633260330208	32.38510089785759	24.63168443255562
11	32.90797565844237	20.872966485909856	26.04433525404278	20.17472260160499
12	24.834530746167335	23.816258414889404	29.551717728444547	21.79749311049871
13	26.904209667121926	22.43027663065	26.33688651112422	24.328627191103855
14	24.27852172718383	21.381294498904953	25.064046097026804	29.27613767688441
15	23.594824590468647	27.773781911927525	23.721704555556453	24.909688942047374
16	23.845351910068775	25.847146008938164	26.353857716645518	23.953644364347536
17	24.312464138226428	25.00101019080484	25.103645576576504	25.58288009439223
18	23.087304730117424	27.05533421152587	26.699747048222466	23.157614010134235
19	25.31295711133919	24.518543062413627	26.242332651791273	23.926167174455912
20	24.645423027501433	25.5440887674864	25.760673676043933	24.04981452896823
21	23.768577408900992	25.449534908153453	26.2302103621332	24.551677320812352
22	23.08326396689807	26.61489102061597	26.885622156312884	23.416222856173075
23	24.378732655023878	25.158599956359755	26.273850604902254	24.188816783714106
24	22.788288251885017	27.373746353211192	26.686008453276656	23.151956941627137
25	23.444508198708572	25.921496052174337	26.432248523101045	24.20174722601605
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	5.5
3	11.0
4	11.0
5	11.0
6	29.5
7	48.0
8	48.0
9	48.0
10	131.0
11	214.0
12	214.0
13	214.0
14	285.0
15	356.0
16	356.0
17	356.0
18	617.5
19	879.0
20	879.0
21	879.0
22	1437.5
23	1996.0
24	1996.0
25	1996.0
26	2885.0
27	3774.0
28	3774.0
29	3774.0
30	4907.5
31	6041.0
32	6041.0
33	6041.0
34	7372.0
35	8703.0
36	8703.0
37	8703.0
38	10255.5
39	11808.0
40	11808.0
41	11808.0
42	13074.0
43	14340.0
44	14340.0
45	14340.0
46	15286.5
47	16233.0
48	16233.0
49	16233.0
50	16071.0
51	15909.0
52	15909.0
53	15909.0
54	15075.0
55	14241.0
56	14241.0
57	14241.0
58	13010.0
59	11779.0
60	11779.0
61	11779.0
62	10551.0
63	9323.0
64	9323.0
65	9323.0
66	7150.5
67	4978.0
68	4978.0
69	4978.0
70	3486.0
71	1994.0
72	1994.0
73	1994.0
74	1381.0
75	768.0
76	768.0
77	768.0
78	515.5
79	263.0
80	263.0
81	263.0
82	160.5
83	58.0
84	58.0
85	58.0
86	39.0
87	20.0
88	20.0
89	20.0
90	11.5
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	123739.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.13696571008332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97276781478318	56.53512635466586
2	10.429120409906064	14.212172395121991
3	2.8287788215200687	5.782332166899684
4	1.2358857576620172	3.3683802196558887
5	0.6962235506215011	2.371928009762484
6	0.41512477464655095	1.6971205521298862
7	0.2894012714678812	1.3803247157323075
8	0.21112059967738875	1.1508093648728372
9	0.16011955593509822	0.9819054623037199
>10	0.7033399753297277	8.207598251157679
>50	0.034396052756428507	1.5791302661246656
>100	0.023721415694088623	2.7331722415729884
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	481	0.3887214217021311	No Hit
CCCTCTCTGATTACAACATCCAGAA	310	0.2505273196001261	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	308	0.24891101431238333	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	215	0.1737528184323455	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	182	0.14708378118459015	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	177	0.14304301796523328	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	164	0.1325370335949054	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	143	0.11556582807360655	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	141	0.11394952278586379	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	137	0.11071691221037828	No Hit
CTTTATCCTGGATCTTGGCCTTCAC	134	0.10829245427876417	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	130	0.10505984370327867	No Hit
TCACAAAGATCTGCATGCCACCTCT	128	0.10344353841553593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGGT	50	2.1332744E-7	19.0	4
CCTAGAG	50	2.1332744E-7	19.0	2
CTAGAGG	55	5.8020123E-7	17.272728	3
AGGGTGA	45	3.496596E-5	16.88889	6
CCCCCCT	65	1.8719038E-7	16.076923	16
TCCCCCC	65	1.8719038E-7	16.076923	15
GATAAAG	50	8.611091E-5	15.2	2
CCCTAGA	70	4.3409273E-7	14.928572	1
TGCCAGT	45	6.7003013E-4	14.777778	19
AGAGGTG	110	1.0913936E-11	14.681818	5
AAAGAGG	60	2.5307821E-5	14.250001	5
GTTGACT	55	1.9373038E-4	13.818182	3
GGGTGAT	55	1.9373038E-4	13.818182	7
AAAGTGT	55	1.9373038E-4	13.818182	6
GGTTGAC	55	1.9373038E-4	13.818182	2
GATGTTG	105	1.3187673E-9	13.571429	17
GGAGCCC	100	9.735231E-9	13.299999	11
GGCGAGA	50	0.0014859859	13.299999	19
AGTGACA	115	3.619789E-10	13.217391	18
GTGACAC	115	3.619789E-10	13.217391	19
>>END_MODULE