FastQCFastQC Report
Mon 15 Aug 2016
SRR2920809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123739
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTT6630.5358052028867213No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5560.4493328699924842No Hit
CCCTAGAGGTGGAGCCCAGTGACAC5330.43074535918344253No Hit
CCCTCTCTGATTACAACATCCAGAA2550.2060789241872005No Hit
GGGTCGACTCTTTCTGGATGTTGTA2380.1923403292413871No Hit
GGATAAAGAGGGCATCCCCCCTGAC1940.15678161291104664No Hit
CTCTCAGGCGAAGGACCAGGTGCAG1800.1454674758968474No Hit
GGACAAGGAGGGCATCCCCCCTGAC1780.14385117060910466No Hit
AGTCCACCCTGCACCTGGTCCTTCG1760.1422348653213619No Hit
TCACAAAGATCTGCATGCCACCTCT1700.13738594945813365No Hit
TCTCTATGGTGTCACTGGGCTCCAC1620.13092072830716267No Hit
GCCTCAGAGGTGGCATGCAGATCTT1530.1236473545123203No Hit
TCTCTATGGTGTCACTGGGCTCGAC1440.11637398071747793No Hit
GGCGAAGGACCAGGTGCAGGGTTGA1390.11233321749812104No Hit
CTTTATCCTGGATCTTGGCCTTCAC1300.10505984370327867No Hit
CCATCGAGAACGTGAAGGCCAAGAT1290.1042516910594073No Hit
GGGTTGACTCCTTCTGGATGTTGTA1260.10182723312779317No Hit
CCACTGTACCGTATGCGAATCTTTG1260.10182723312779317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATTA250.00600143319.08
ACGCAGA757.2759576E-1219.01
CTGCATG359.845875E-519.011
GCAGAGT853.6379788E-1217.8823533
CGCAGAG802.1827873E-1117.81252
GATCTGC504.4942262E-617.0999988
AGATCTG453.496596E-516.888897
CAGAGTA907.2759576E-1216.888894
TGCATGC402.7354324E-416.62512
GTGACAC1100.015.54545519
CTAGAGG755.6974386E-815.2000013
AGAGTAC952.928573E-1015.05
ACAACAT704.3409273E-714.92857213
TAGAGGT704.3409273E-714.9285724
CCCTAGA704.3409273E-714.9285721
TGATTAC704.3409273E-714.9285728
TACAACA704.3409273E-714.92857212
GCATGCC456.7003013E-414.77777813
TCTGCAT653.3119322E-614.61538610
TCTAGGG400.00524284614.253
Produced by FastQC (version 0.11.5)