##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123739
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67958363975788	33.0	32.0	34.0	32.0	34.0
2	32.93521040254083	33.0	32.0	34.0	32.0	34.0
3	33.13066211946112	33.0	33.0	34.0	32.0	34.0
4	33.19849845238769	33.0	33.0	34.0	32.0	34.0
5	32.97839807982932	33.0	33.0	34.0	32.0	34.0
6	35.7812492423569	37.0	35.0	37.0	33.0	37.0
7	35.87701533065565	37.0	37.0	37.0	33.0	37.0
8	35.82340248426123	37.0	35.0	37.0	33.0	37.0
9	35.75654401603375	37.0	35.0	37.0	33.0	37.0
10	35.721171174811495	37.0	35.0	37.0	33.0	37.0
11	35.71135212018846	37.0	35.0	37.0	33.0	37.0
12	35.62784570749723	37.0	34.0	37.0	33.0	37.0
13	37.29202595786292	38.0	38.0	38.0	37.0	38.0
14	37.43562660115243	38.0	38.0	38.0	37.0	38.0
15	37.44464558465803	38.0	38.0	38.0	37.0	38.0
16	37.41409741471969	38.0	38.0	38.0	37.0	38.0
17	37.42410234445082	38.0	38.0	38.0	37.0	38.0
18	37.391073145895795	38.0	38.0	38.0	37.0	38.0
19	37.38371895683657	38.0	38.0	38.0	37.0	38.0
20	37.39942944423343	38.0	38.0	38.0	37.0	38.0
21	37.401813494532846	38.0	38.0	38.0	37.0	38.0
22	37.43197375120213	38.0	38.0	38.0	37.0	38.0
23	38.0731458957968	39.0	38.0	39.0	37.0	39.0
24	38.0644420918223	39.0	38.0	39.0	37.0	39.0
25	38.008259320020365	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	9.0
24	27.0
25	46.0
26	120.0
27	224.0
28	393.0
29	651.0
30	1013.0
31	1398.0
32	1911.0
33	2925.0
34	4976.0
35	14286.0
36	76816.0
37	18943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.53181292882599	16.169518098578457	10.191612991861902	27.10705598073364
2	19.983190425007475	19.89186917625001	32.99363983869274	27.13130056004978
3	20.45595972167223	22.591099006780404	30.064894657302872	26.888046614244498
4	13.316739265712506	16.119412634658435	37.4918174544808	33.07203064514825
5	13.295727296971851	32.33176282336208	32.34630957095176	22.026200308714312
6	30.484325879472113	33.07122249250438	20.092291031930113	16.35216059609339
7	26.856528661133517	30.388155714851422	22.918400827548307	19.836914796466758
8	27.435974106789292	30.69525371952254	21.68920065621995	20.17957151746822
9	25.5101463564438	18.7459087272404	22.23793630140861	33.50600861490719
10	17.40599164370166	25.85603568802075	32.28165735944205	24.45631530883553
11	32.66552986528096	20.47858799570063	26.1849538140764	20.670928324942015
12	24.717348612805985	23.21418469520523	30.224908880789403	21.84355781119938
13	26.73045684868958	22.070648704127237	26.909058582985157	24.289835864198032
14	24.367418518009682	20.96186327673571	25.80027315559363	28.87044504966098
15	23.655436038759	27.297780004687283	24.44338486653359	24.603399090020122
16	23.54795173712411	25.629752947736765	27.02300810577102	23.799287209368106
17	24.046581918392747	24.556526236675584	26.024939590589867	25.3719522543418
18	22.953959543878646	26.578524151641762	27.41172952747315	23.05578677700644
19	24.794123113973768	24.43772779802649	26.938960230808394	23.829188857191347
20	24.45954791941102	25.355789201464376	26.562361098764338	23.622301780360274
21	23.606138727482847	25.241839678678506	26.797533518130905	24.35448807570774
22	22.94426171215219	26.4419463548275	27.469916517831887	23.14387541518842
23	24.150025456808283	24.818367693289908	26.96562926805615	24.06597758184566
24	22.781823030734046	27.134533170625268	27.12321903361107	22.960424765029618
25	23.31924453890851	25.46084904516765	27.256564219849842	23.963342196073995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	7.0
3	13.0
4	13.0
5	13.0
6	24.5
7	36.0
8	36.0
9	36.0
10	379.5
11	723.0
12	723.0
13	723.0
14	543.5
15	364.0
16	364.0
17	364.0
18	612.0
19	860.0
20	860.0
21	860.0
22	1671.5
23	2483.0
24	2483.0
25	2483.0
26	3073.0
27	3663.0
28	3663.0
29	3663.0
30	4887.0
31	6111.0
32	6111.0
33	6111.0
34	7271.5
35	8432.0
36	8432.0
37	8432.0
38	10015.5
39	11599.0
40	11599.0
41	11599.0
42	13050.0
43	14501.0
44	14501.0
45	14501.0
46	15318.5
47	16136.0
48	16136.0
49	16136.0
50	15863.5
51	15591.0
52	15591.0
53	15591.0
54	14996.0
55	14401.0
56	14401.0
57	14401.0
58	13086.5
59	11772.0
60	11772.0
61	11772.0
62	10555.5
63	9339.0
64	9339.0
65	9339.0
66	7052.5
67	4766.0
68	4766.0
69	4766.0
70	3324.0
71	1882.0
72	1882.0
73	1882.0
74	1299.5
75	717.0
76	717.0
77	717.0
78	482.5
79	248.0
80	248.0
81	248.0
82	161.5
83	75.0
84	75.0
85	75.0
86	45.5
87	16.0
88	16.0
89	16.0
90	12.0
91	8.0
92	8.0
93	8.0
94	5.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	123739.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.94978947623628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.49097690812742	55.22753537688199
2	10.69734316719578	14.32369745997624
3	2.9175669640162716	5.859914820711336
4	1.301256593797907	3.484754200373367
5	0.7339183757227528	2.456784037368978
6	0.41765749671064545	1.6777248886769731
7	0.3078111608705624	1.4425524693104033
8	0.18830800429728523	1.0085744995514754
9	0.16054464468935215	0.9673587147140351
>10	0.7266757601728571	8.362763558780982
>50	0.03017756479123161	1.320521420085826
>100	0.024142051832985286	2.45193512150575
>500	0.0036213077749477933	1.415883432062648
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACTTTTTTTTTTTTTT	663	0.5358052028867213	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	556	0.4493328699924842	No Hit
CCCTAGAGGTGGAGCCCAGTGACAC	533	0.43074535918344253	No Hit
CCCTCTCTGATTACAACATCCAGAA	255	0.2060789241872005	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	238	0.1923403292413871	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	194	0.15678161291104664	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	180	0.1454674758968474	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	178	0.14385117060910466	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	176	0.1422348653213619	No Hit
TCACAAAGATCTGCATGCCACCTCT	170	0.13738594945813365	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	162	0.13092072830716267	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	153	0.1236473545123203	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	144	0.11637398071747793	No Hit
GGCGAAGGACCAGGTGCAGGGTTGA	139	0.11233321749812104	No Hit
CTTTATCCTGGATCTTGGCCTTCAC	130	0.10505984370327867	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	129	0.1042516910594073	No Hit
GGGTTGACTCCTTCTGGATGTTGTA	126	0.10182723312779317	No Hit
CCACTGTACCGTATGCGAATCTTTG	126	0.10182723312779317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATTA	25	0.006001433	19.0	8
ACGCAGA	75	7.2759576E-12	19.0	1
CTGCATG	35	9.845875E-5	19.0	11
GCAGAGT	85	3.6379788E-12	17.882353	3
CGCAGAG	80	2.1827873E-11	17.8125	2
GATCTGC	50	4.4942262E-6	17.099998	8
AGATCTG	45	3.496596E-5	16.88889	7
CAGAGTA	90	7.2759576E-12	16.88889	4
TGCATGC	40	2.7354324E-4	16.625	12
GTGACAC	110	0.0	15.545455	19
CTAGAGG	75	5.6974386E-8	15.200001	3
AGAGTAC	95	2.928573E-10	15.0	5
ACAACAT	70	4.3409273E-7	14.928572	13
TAGAGGT	70	4.3409273E-7	14.928572	4
CCCTAGA	70	4.3409273E-7	14.928572	1
TGATTAC	70	4.3409273E-7	14.928572	8
TACAACA	70	4.3409273E-7	14.928572	12
GCATGCC	45	6.7003013E-4	14.777778	13
TCTGCAT	65	3.3119322E-6	14.615386	10
TCTAGGG	40	0.005242846	14.25	3
>>END_MODULE