FastQCFastQC Report
Mon 15 Aug 2016
SRR2920808_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920808_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200318
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC15210.759292724567937No Hit
CCCTCTCTGATTACAACATCCAGAA9180.4582713485557963No Hit
GGGTCGACTCTTTCTGGATGTTGTA7870.39287532822811727No Hit
AGTCCACCCTGCACCTGGTCCTTCG6400.31949200770774466No Hit
GGACAAGGAGGGCATCCCCCCTGAC6210.310007088728921No Hit
CTCTCAGGCGAAGGACCAGGTGCAG5540.2765602691720165No Hit
GGATAAAGAGGGCATCCCCCCTGAC5420.2705697940274963No Hit
TCTCTATGGTGTCACTGGGCTCCAC5110.2550943999041524No Hit
TCACAAAGATCTGCATGCCACCTCT4670.23312932437424494No Hit
GCCTCAGAGGTGGCATGCAGATCTT4450.2221467866092912No Hit
TCTCTATGGTGTCACTGGGCTCGAC4300.21465869267864096No Hit
CCTCCAGGGTGATGGTCTTGCCTGT4240.21166345510638085No Hit
GGGTTGACTCTTTCTGGATGTTGTA4210.21016583632025082No Hit
GGGTTGACTCCTTCTGGATGTTGTA3860.1926936171487335No Hit
CCATCGAGAACGTGAAGGCCAAGAT3840.19169520462464681No Hit
CTTTATCCTGGATCTTGGCCTTCAC3500.17472219171517286No Hit
GAGTCCACCCTGCACCTGGTCCTTC3180.15874759132978564No Hit
CCCTGGAGGTGGAGCCCAGTGACAC3130.15625156001956889No Hit
TGCCTGCAAAGATCAGCCTCTGCTG2680.13378727822761807No Hit
GGCGAAGGACCAGGTGCAGGGTTGA2580.12879521560718457No Hit
GCTCCACCTCCAGGGTGATGGTCTT2560.12779680308309788No Hit
CTCTTTATCCTGGATCTTGGCCTTC2450.122305534200621No Hit
CCTCTCAGGCGAAGGACCAGGTGCA2380.11881109036631755No Hit
AGGACAAGGAGGGCATCCCCCCTGA2360.11781267784223085No Hit
TTGCCGGCAAGCAGCTGGAAGATGG2180.10882696512545054No Hit
CCTCTGAGGCGAAGGACCAGGTGCA2130.10633093381523379No Hit
CCTCCAGGGTGATGGTCTTGCCAGT2080.10383490250501703No Hit
AGGCCAAGATCCAGGACAAGGAGGG2040.10183807745684362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG250.00601415419.013
ATCCGTA250.00601415419.015
TCCGTAG250.00601415419.016
CGAGAAT250.00601415419.05
CATCGAG900.017.9444452
TAGAGGT1650.017.2727264
CCATCGA951.8189894E-1217.01
TAACACA402.746082E-416.6254
AGATCTG1050.016.2857157
CTGCATG1050.016.28571511
GTAACAC350.002161336416.2857153
TATCCGT350.002161336416.28571514
TCGAGAA1001.8189894E-1216.154
ATCGAGA1001.8189894E-1216.153
CAGGCGA951.8189894E-1116.05
CTAGAGG1850.015.918923
TGCATGC1150.015.69565212
CCTAGAG1900.015.52
GAGAACG755.7583748E-815.26
CCCTAGA1950.015.1025641
Produced by FastQC (version 0.11.5)