##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920808_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200318
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276510348545813	32.0	32.0	33.0	29.0	34.0
2	31.8477470821394	33.0	32.0	33.0	29.0	34.0
3	32.205233678451265	33.0	32.0	34.0	32.0	34.0
4	32.36507952355754	33.0	32.0	34.0	32.0	34.0
5	32.530661248614706	33.0	32.0	34.0	32.0	34.0
6	35.3788725925778	37.0	34.0	37.0	33.0	37.0
7	35.39789734322427	37.0	34.0	37.0	33.0	37.0
8	35.27075450034445	37.0	34.0	37.0	33.0	37.0
9	35.32188819776555	37.0	34.0	37.0	33.0	37.0
10	35.357940874010325	37.0	34.0	37.0	33.0	37.0
11	35.266071945606484	37.0	34.0	37.0	33.0	37.0
12	35.23314430056211	37.0	34.0	37.0	33.0	37.0
13	36.97459040126199	38.0	38.0	38.0	36.0	38.0
14	37.10640581475454	38.0	38.0	38.0	37.0	38.0
15	37.09196876965625	38.0	38.0	38.0	37.0	38.0
16	36.98457452650286	38.0	38.0	38.0	36.0	38.0
17	36.97790513084196	38.0	38.0	38.0	36.0	38.0
18	37.077242184925964	38.0	38.0	38.0	37.0	38.0
19	37.05856188660031	38.0	38.0	38.0	37.0	38.0
20	36.99206761249613	38.0	38.0	38.0	36.0	38.0
21	37.01919448077557	38.0	38.0	38.0	36.0	38.0
22	37.05483281582284	38.0	38.0	38.0	37.0	38.0
23	37.579398755978	39.0	38.0	39.0	36.0	39.0
24	37.43995047873881	39.0	38.0	39.0	36.0	39.0
25	37.417571062011405	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	12.0
19	47.0
20	83.0
21	201.0
22	304.0
23	443.0
24	631.0
25	853.0
26	1002.0
27	1292.0
28	1609.0
29	1950.0
30	2450.0
31	2899.0
32	3916.0
33	5772.0
34	10505.0
35	32398.0
36	120740.0
37	13205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.526612685829534	15.039087850317994	11.34396309867311	28.090336365179365
2	20.935712217574057	17.867091324793577	32.50731337173893	28.68988308589343
3	20.253796463622837	21.392985153605768	29.043321119420124	29.30989726335127
4	13.264908795015925	14.975189448776446	36.32574207010853	35.4341596860991
5	13.857965834323426	31.630207969328765	30.825487474914887	23.68633872143292
6	32.38051498117993	33.2945616469813	18.707754670074582	15.617168701764195
7	28.53013708203956	30.127097914316238	20.829880490020866	20.512884513623337
8	29.51956389340948	29.44168771653072	19.816491778072866	21.222256611986943
9	27.28960952086183	17.924000838666522	20.189398855819245	34.596990784652405
10	17.947463532982557	24.25343703511417	32.12741740632394	25.67168202557933
11	35.67327948561787	19.13657284916982	24.743158378178695	20.446989287033617
12	26.662107249473337	22.53017701854052	28.61400373406284	22.1937119979233
13	27.874180053714593	21.638095428269054	25.287792410067993	25.199932107948364
14	26.481893788875688	19.721642588284627	22.623528589542627	31.172935033297055
15	24.78109805409399	26.615181860841265	22.443814335207023	26.159905749857725
16	24.792579798120986	24.086202937329645	25.95323435737178	25.16798290717759
17	26.341117622979464	22.264100080871415	23.7931688615102	27.60161343463892
18	24.79607424195529	26.217314469992715	24.651803632224762	24.334807655827234
19	26.717519144560146	22.755818248984117	25.1934424265418	25.333220179913933
20	26.538803302748633	23.513613354765923	24.113160075479986	25.834423267005462
21	24.953324214498945	23.67435777114388	25.2568416218213	26.115476392535868
22	23.67136253357162	25.624756636947254	26.006649427410416	24.697231402070706
23	26.60020567297996	22.620034145708324	25.01023372837189	25.76952645293983
24	23.95391327788816	27.098413522499225	25.24585908405635	23.701814115556267
25	24.56643936141535	24.791082179334857	24.909394063439134	25.733084395810664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	6.0
3	9.0
4	9.0
5	9.0
6	24.0
7	39.0
8	39.0
9	39.0
10	121.5
11	204.0
12	204.0
13	204.0
14	299.5
15	395.0
16	395.0
17	395.0
18	618.0
19	841.0
20	841.0
21	841.0
22	1501.0
23	2161.0
24	2161.0
25	2161.0
26	3081.0
27	4001.0
28	4001.0
29	4001.0
30	5458.0
31	6915.0
32	6915.0
33	6915.0
34	8549.5
35	10184.0
36	10184.0
37	10184.0
38	12772.5
39	15361.0
40	15361.0
41	15361.0
42	18110.5
43	20860.0
44	20860.0
45	20860.0
46	23335.5
47	25811.0
48	25811.0
49	25811.0
50	26060.0
51	26309.0
52	26309.0
53	26309.0
54	26404.0
55	26499.0
56	26499.0
57	26499.0
58	24834.5
59	23170.0
60	23170.0
61	23170.0
62	21625.5
63	20081.0
64	20081.0
65	20081.0
66	15439.5
67	10798.0
68	10798.0
69	10798.0
70	7507.0
71	4216.0
72	4216.0
73	4216.0
74	2929.5
75	1643.0
76	1643.0
77	1643.0
78	1088.5
79	534.0
80	534.0
81	534.0
82	369.0
83	204.0
84	204.0
85	204.0
86	134.0
87	64.0
88	64.0
89	64.0
90	38.0
91	12.0
92	12.0
93	12.0
94	8.0
95	4.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	200318.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.37412068021959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46662675082456	38.7703159002595
2	12.940935995632803	13.296584151092746
3	4.307461156407539	6.638760878239175
4	2.183000987008796	4.485990246065135
5	1.279994002259164	3.287928317100978
6	0.8439958362733048	2.6015726367784566
7	0.5809971268065145	2.0893751555193107
8	0.41299795737206046	1.6973925522173139
9	0.28099861021388717	1.2992450860892002
>10	1.5599922844592666	13.98807639540821
>50	0.07699961916882202	2.8065543318684676
>100	0.05799971314015165	5.907480919303588
>500	0.00699996537898382	2.349326919208219
>1k	9.999950541405458E-4	0.7813965108497051
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	1521	0.759292724567937	No Hit
CCCTCTCTGATTACAACATCCAGAA	918	0.4582713485557963	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	787	0.39287532822811727	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	640	0.31949200770774466	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	621	0.310007088728921	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	554	0.2765602691720165	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	542	0.2705697940274963	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	511	0.2550943999041524	No Hit
TCACAAAGATCTGCATGCCACCTCT	467	0.23312932437424494	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	445	0.2221467866092912	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	430	0.21465869267864096	No Hit
CCTCCAGGGTGATGGTCTTGCCTGT	424	0.21166345510638085	No Hit
GGGTTGACTCTTTCTGGATGTTGTA	421	0.21016583632025082	No Hit
GGGTTGACTCCTTCTGGATGTTGTA	386	0.1926936171487335	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	384	0.19169520462464681	No Hit
CTTTATCCTGGATCTTGGCCTTCAC	350	0.17472219171517286	No Hit
GAGTCCACCCTGCACCTGGTCCTTC	318	0.15874759132978564	No Hit
CCCTGGAGGTGGAGCCCAGTGACAC	313	0.15625156001956889	No Hit
TGCCTGCAAAGATCAGCCTCTGCTG	268	0.13378727822761807	No Hit
GGCGAAGGACCAGGTGCAGGGTTGA	258	0.12879521560718457	No Hit
GCTCCACCTCCAGGGTGATGGTCTT	256	0.12779680308309788	No Hit
CTCTTTATCCTGGATCTTGGCCTTC	245	0.122305534200621	No Hit
CCTCTCAGGCGAAGGACCAGGTGCA	238	0.11881109036631755	No Hit
AGGACAAGGAGGGCATCCCCCCTGA	236	0.11781267784223085	No Hit
TTGCCGGCAAGCAGCTGGAAGATGG	218	0.10882696512545054	No Hit
CCTCTGAGGCGAAGGACCAGGTGCA	213	0.10633093381523379	No Hit
CCTCCAGGGTGATGGTCTTGCCAGT	208	0.10383490250501703	No Hit
AGGCCAAGATCCAGGACAAGGAGGG	204	0.10183807745684362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	25	0.006014154	19.0	13
ATCCGTA	25	0.006014154	19.0	15
TCCGTAG	25	0.006014154	19.0	16
CGAGAAT	25	0.006014154	19.0	5
CATCGAG	90	0.0	17.944445	2
TAGAGGT	165	0.0	17.272726	4
CCATCGA	95	1.8189894E-12	17.0	1
TAACACA	40	2.746082E-4	16.625	4
AGATCTG	105	0.0	16.285715	7
CTGCATG	105	0.0	16.285715	11
GTAACAC	35	0.0021613364	16.285715	3
TATCCGT	35	0.0021613364	16.285715	14
TCGAGAA	100	1.8189894E-12	16.15	4
ATCGAGA	100	1.8189894E-12	16.15	3
CAGGCGA	95	1.8189894E-11	16.0	5
CTAGAGG	185	0.0	15.91892	3
TGCATGC	115	0.0	15.695652	12
CCTAGAG	190	0.0	15.5	2
GAGAACG	75	5.7583748E-8	15.2	6
CCCTAGA	195	0.0	15.102564	1
>>END_MODULE