FastQCFastQC Report
Mon 15 Aug 2016
SRR2920808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200318
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACAC16290.8132070008686189No Hit
CCCTCTCTGATTACAACATCCAGAA9150.45677372976966624No Hit
CTCTCAGGCGAAGGACCAGGTGCAG6840.34145708323765217No Hit
AGTCCACCCTGCACCTGGTCCTTCG6680.3334697830449585No Hit
GGGTCGACTCTTTCTGGATGTTGTA6580.328477720424525No Hit
GGACAAGGAGGGCATCCCCCCTGAC5790.28904042572310024No Hit
TCTCTATGGTGTCACTGGGCTCCAC5150.2570912249523258No Hit
GCCTCAGAGGTGGCATGCAGATCTT5040.251599956069849No Hit
GGATAAAGAGGGCATCCCCCCTGAC4800.2396190057808085No Hit
TCTCTATGGTGTCACTGGGCTCGAC4620.2306332930640282No Hit
TCACAAAGATCTGCATGCCACCTCT4480.22364440539542127No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4330.21615631146477102No Hit
CCTCCAGGGTGATGGTCTTGCCTGT4020.20068091734142712No Hit
CCATCGAGAACGTGAAGGCCAAGAT3950.19718647350712368No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3780.1886999670523867No Hit
GGGTTGACTCCTTCTGGATGTTGTA3670.18320869816990984No Hit
GGGTTGACTCTTTCTGGATGTTGTA3670.18320869816990984No Hit
CTTTATCCTGGATCTTGGCCTTCAC3610.18021346059764976No Hit
CCCTGGAGGTGGAGCCCAGTGACAC3250.16224203516408908No Hit
CCTCTCAGGCGAAGGACCAGGTGCA2880.1437714034684851No Hit
TGCCTGCAAAGATCAGCCTCTGCTG2790.13927854711009494No Hit
GAGTCCACCCTGCACCTGGTCCTTC2740.13678251579987818No Hit
GCTCCACCTCCAGGGTGATGGTCTT2720.1357841032757915No Hit
AGGACAAGGAGGGCATCCCCCCTGA2650.13228965944148804No Hit
GGCGAAGGACCAGGTGCAGGGTTGA2630.13129124691740132No Hit
CTCTTTATCCTGGATCTTGGCCTTC2520.1257999780349245No Hit
TTGCCGGCAAGCAGCTGGAAGATGG2480.12380315298675107No Hit
AGGCCAAGATCCAGGACAAGGAGGG2280.11381902774588405No Hit
GGCGAAGGACCAGGTGCAGGGTGGA2190.10932617138749388No Hit
CCTCTGAGGCGAAGGACCAGGTGCA2040.10183807745684362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC250.00601415419.03
TAGAGGT1650.018.4242424
CTAGAGG1600.018.406253
CCCTAGA1950.017.0512811
CCTAGAG1900.017.02
GTCGACT651.8890023E-716.0769233
GGTCGAC651.8890023E-716.0769232
GGGTCGA704.3801992E-714.9285721
TCGACTC704.3801992E-714.9285724
GTGACAC2950.014.81355919
GGCAAGC602.5462992E-514.2500016
AAGATCT1152.3646862E-1114.0434786
TATGGTG1101.8189894E-1013.8181825
GTCCACC1101.8189894E-1013.8181822
AGGTGGA2850.013.6666677
ACGCAGA707.187309E-613.5714281
CCTCCAA707.187309E-613.5714281
AGTGACA3150.013.57142718
CAGTGAC3200.013.35937517
TGCCACC1009.8734745E-913.29999916
Produced by FastQC (version 0.11.5)