##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200318
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79841551932427	33.0	32.0	34.0	32.0	34.0
2	33.04945137231802	33.0	33.0	34.0	32.0	34.0
3	33.24483571121916	33.0	33.0	34.0	32.0	35.0
4	33.31196397727613	34.0	33.0	34.0	32.0	35.0
5	33.10063499036532	34.0	33.0	34.0	32.0	35.0
6	35.76404017611997	37.0	35.0	37.0	33.0	37.0
7	35.866741880410146	37.0	35.0	37.0	33.0	37.0
8	35.81584780199483	37.0	35.0	37.0	33.0	37.0
9	35.72152777084436	37.0	35.0	37.0	33.0	37.0
10	35.668047803991655	37.0	35.0	37.0	33.0	37.0
11	35.640995816651525	37.0	34.0	37.0	33.0	37.0
12	35.554578220629196	37.0	34.0	37.0	33.0	37.0
13	37.349788835751156	38.0	38.0	38.0	37.0	38.0
14	37.472967980910354	38.0	38.0	38.0	37.0	38.0
15	37.48813386715123	38.0	38.0	38.0	37.0	38.0
16	37.462150181211875	38.0	38.0	38.0	37.0	38.0
17	37.461685919388174	38.0	38.0	38.0	37.0	38.0
18	37.43045058357212	38.0	38.0	38.0	37.0	38.0
19	37.420611228147244	38.0	38.0	38.0	37.0	38.0
20	37.42895795684861	38.0	38.0	38.0	37.0	38.0
21	37.43339590051818	38.0	38.0	38.0	37.0	38.0
22	37.46338821274174	38.0	38.0	38.0	37.0	38.0
23	38.10226240277958	39.0	38.0	39.0	37.0	39.0
24	38.09205363472079	39.0	38.0	39.0	37.0	39.0
25	38.04896714224383	39.0	38.0	39.0	37.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	2.0
23	16.0
24	41.0
25	98.0
26	188.0
27	323.0
28	618.0
29	989.0
30	1401.0
31	2079.0
32	3021.0
33	4350.0
34	7777.0
35	22547.0
36	124774.0
37	32093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86666200740822	15.31564811949001	11.641490030850948	28.17619984225082
2	20.8049201769187	17.86908814984175	32.18183088888667	29.14416078435288
3	20.65465909204365	21.014586806976908	28.666919597839435	29.66383450314001
4	13.297357202048744	15.116464820934713	36.18346828542617	35.402709691590374
5	13.66177777334039	31.862338881178925	30.52197006759253	23.95391327788816
6	32.529278447268844	33.43483860661548	18.29640871015086	15.739474235964815
7	28.6055172276081	30.065695544084903	20.96915903713096	20.35962819117603
8	29.958366197745583	29.458161523178145	19.73112750726345	20.852344771812817
9	27.346519034734772	17.97641749618107	20.292235345800176	34.38482812328398
10	17.829151648878284	24.755139328467738	32.05353487954153	25.362174143112455
11	35.71321598658134	18.842041154564242	24.868459149951576	20.576283708902846
12	26.618676304675564	22.531175431064607	28.681396579438694	22.168751684821135
13	27.99249193781887	21.447897842430535	25.338212242534368	25.221397977216224
14	26.359588254675064	19.533441827494283	23.12922453299254	30.97774538483811
15	24.599886180972256	26.694555656506154	22.657474615361576	26.04808354716002
16	24.750147265847303	23.828612506115277	26.35659301710281	25.064647210934616
17	26.234287482902186	22.30803023193123	23.847582344072922	27.61009994109366
18	24.695234577022536	26.26623668367296	24.823031380105633	24.215497359198874
19	26.698549306602505	22.803742050140276	25.312752723170163	25.18495592008706
20	26.59521361035953	23.44372447807985	24.425663195519125	25.535398716041495
21	25.07013847981709	23.53857366786809	25.462514601783166	25.928773250531655
22	23.736259347637258	25.683662975868373	26.056570053614752	24.52350762287962
23	26.40152158068671	22.66995477191266	25.312253516908118	25.61627013049252
24	24.070727543206303	27.12836589822183	25.2568416218213	23.544064936750566
25	24.55296079234018	24.611867131261295	25.281302728661426	25.553869347737095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	3.5
3	5.0
4	5.0
5	5.0
6	20.5
7	36.0
8	36.0
9	36.0
10	281.0
11	526.0
12	526.0
13	526.0
14	432.5
15	339.0
16	339.0
17	339.0
18	591.0
19	843.0
20	843.0
21	843.0
22	1636.5
23	2430.0
24	2430.0
25	2430.0
26	3264.0
27	4098.0
28	4098.0
29	4098.0
30	5398.0
31	6698.0
32	6698.0
33	6698.0
34	8377.5
35	10057.0
36	10057.0
37	10057.0
38	12541.5
39	15026.0
40	15026.0
41	15026.0
42	17946.5
43	20867.0
44	20867.0
45	20867.0
46	23184.5
47	25502.0
48	25502.0
49	25502.0
50	26070.0
51	26638.0
52	26638.0
53	26638.0
54	26670.0
55	26702.0
56	26702.0
57	26702.0
58	24906.5
59	23111.0
60	23111.0
61	23111.0
62	21753.5
63	20396.0
64	20396.0
65	20396.0
66	15525.0
67	10654.0
68	10654.0
69	10654.0
70	7402.0
71	4150.0
72	4150.0
73	4150.0
74	2817.5
75	1485.0
76	1485.0
77	1485.0
78	983.5
79	482.0
80	482.0
81	482.0
82	340.0
83	198.0
84	198.0
85	198.0
86	129.0
87	60.0
88	60.0
89	60.0
90	35.0
91	10.0
92	10.0
93	10.0
94	5.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	200318.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.73100682692768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92492355887728	38.01016808572438
2	13.207986524733414	13.401089091124394
3	4.509096040392869	6.862529460253297
4	2.1639991516275963	4.391274229387411
5	1.3029986936678901	3.3051217811971796
6	0.8609991220057379	2.6207611401871103
7	0.5509994378581117	1.9566929370529225
8	0.4369995541573875	1.7735541892258222
9	0.3169996765854436	1.4473541481291006
>10	1.5679984002711989	13.59995443499632
>50	0.08899990919906678	3.134665713736282
>100	0.05999993878588771	6.375866433118239
>500	0.006999992858353566	2.2945610977875392
>1k	9.999989797647953E-4	0.8264072580800136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACAC	1629	0.8132070008686189	No Hit
CCCTCTCTGATTACAACATCCAGAA	915	0.45677372976966624	No Hit
CTCTCAGGCGAAGGACCAGGTGCAG	684	0.34145708323765217	No Hit
AGTCCACCCTGCACCTGGTCCTTCG	668	0.3334697830449585	No Hit
GGGTCGACTCTTTCTGGATGTTGTA	658	0.328477720424525	No Hit
GGACAAGGAGGGCATCCCCCCTGAC	579	0.28904042572310024	No Hit
TCTCTATGGTGTCACTGGGCTCCAC	515	0.2570912249523258	No Hit
GCCTCAGAGGTGGCATGCAGATCTT	504	0.251599956069849	No Hit
GGATAAAGAGGGCATCCCCCCTGAC	480	0.2396190057808085	No Hit
TCTCTATGGTGTCACTGGGCTCGAC	462	0.2306332930640282	No Hit
TCACAAAGATCTGCATGCCACCTCT	448	0.22364440539542127	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	433	0.21615631146477102	No Hit
CCTCCAGGGTGATGGTCTTGCCTGT	402	0.20068091734142712	No Hit
CCATCGAGAACGTGAAGGCCAAGAT	395	0.19718647350712368	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	378	0.1886999670523867	No Hit
GGGTTGACTCCTTCTGGATGTTGTA	367	0.18320869816990984	No Hit
GGGTTGACTCTTTCTGGATGTTGTA	367	0.18320869816990984	No Hit
CTTTATCCTGGATCTTGGCCTTCAC	361	0.18021346059764976	No Hit
CCCTGGAGGTGGAGCCCAGTGACAC	325	0.16224203516408908	No Hit
CCTCTCAGGCGAAGGACCAGGTGCA	288	0.1437714034684851	No Hit
TGCCTGCAAAGATCAGCCTCTGCTG	279	0.13927854711009494	No Hit
GAGTCCACCCTGCACCTGGTCCTTC	274	0.13678251579987818	No Hit
GCTCCACCTCCAGGGTGATGGTCTT	272	0.1357841032757915	No Hit
AGGACAAGGAGGGCATCCCCCCTGA	265	0.13228965944148804	No Hit
GGCGAAGGACCAGGTGCAGGGTTGA	263	0.13129124691740132	No Hit
CTCTTTATCCTGGATCTTGGCCTTC	252	0.1257999780349245	No Hit
TTGCCGGCAAGCAGCTGGAAGATGG	248	0.12380315298675107	No Hit
AGGCCAAGATCCAGGACAAGGAGGG	228	0.11381902774588405	No Hit
GGCGAAGGACCAGGTGCAGGGTGGA	219	0.10932617138749388	No Hit
CCTCTGAGGCGAAGGACCAGGTGCA	204	0.10183807745684362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	25	0.006014154	19.0	3
TAGAGGT	165	0.0	18.424242	4
CTAGAGG	160	0.0	18.40625	3
CCCTAGA	195	0.0	17.051281	1
CCTAGAG	190	0.0	17.0	2
GTCGACT	65	1.8890023E-7	16.076923	3
GGTCGAC	65	1.8890023E-7	16.076923	2
GGGTCGA	70	4.3801992E-7	14.928572	1
TCGACTC	70	4.3801992E-7	14.928572	4
GTGACAC	295	0.0	14.813559	19
GGCAAGC	60	2.5462992E-5	14.250001	6
AAGATCT	115	2.3646862E-11	14.043478	6
TATGGTG	110	1.8189894E-10	13.818182	5
GTCCACC	110	1.8189894E-10	13.818182	2
AGGTGGA	285	0.0	13.666667	7
ACGCAGA	70	7.187309E-6	13.571428	1
CCTCCAA	70	7.187309E-6	13.571428	1
AGTGACA	315	0.0	13.571427	18
CAGTGAC	320	0.0	13.359375	17
TGCCACC	100	9.8734745E-9	13.299999	16
>>END_MODULE