FastQCFastQC Report
Mon 15 Aug 2016
SRR2920807_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920807_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450740
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTC555.8867226E-717.2727288
TTAGGAG350.002166986716.2857133
AGGGACG551.1247666E-515.5454555
ACGCTCT653.3593042E-614.6153869
ATAGCAC551.9552972E-413.8181823
GTATAAT500.001496668313.2999991
CTAGCAC500.001496668313.2999993
TAGCACC655.435752E-513.1538474
TAGCACA655.435752E-513.1538474
CGCTCTC655.435752E-513.15384710
TATGTTG957.3410774E-813.05
GGACGCT751.4730769E-512.6666667
GGGACGC701.08759064E-412.2142856
GTTTAAT550.003062638512.0909091
ACCGTGA550.003062638512.09090912
AGTACCA550.003062638512.0909095
GTAGGGA802.8557957E-511.8753
TAGGGAC802.8557957E-511.8754
TGAGCTA802.8557957E-511.87518
TGCTCTA752.067594E-411.410
Produced by FastQC (version 0.11.5)