##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920807_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450740
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.619057549806985	33.0	32.0	33.0	29.0	34.0
2	32.026028309002974	33.0	32.0	34.0	30.0	34.0
3	32.29423836357989	33.0	32.0	34.0	32.0	34.0
4	32.43507565337001	33.0	32.0	34.0	32.0	34.0
5	32.54491946576741	33.0	32.0	34.0	32.0	34.0
6	35.17830678439899	37.0	34.0	37.0	33.0	37.0
7	35.20529573590096	37.0	34.0	37.0	33.0	37.0
8	35.30203665084084	37.0	34.0	37.0	33.0	37.0
9	35.284691840085195	37.0	34.0	37.0	33.0	37.0
10	35.2888538847229	37.0	34.0	37.0	33.0	37.0
11	35.22155566401917	37.0	34.0	37.0	33.0	37.0
12	35.15480764964281	37.0	34.0	37.0	33.0	37.0
13	36.98508009051781	38.0	38.0	38.0	36.0	38.0
14	37.01791054710032	38.0	38.0	38.0	36.0	38.0
15	36.97562452855305	38.0	38.0	38.0	36.0	38.0
16	36.95103385543772	38.0	38.0	38.0	36.0	38.0
17	36.9436282557572	38.0	38.0	38.0	36.0	38.0
18	36.92935395128012	38.0	38.0	38.0	36.0	38.0
19	36.96076452056618	38.0	38.0	38.0	36.0	38.0
20	36.97462173314993	38.0	38.0	38.0	36.0	38.0
21	37.012523849669435	38.0	38.0	38.0	36.0	38.0
22	36.88245108044549	38.0	38.0	38.0	36.0	38.0
23	37.49170918933309	39.0	38.0	39.0	36.0	39.0
24	37.53332297998846	39.0	38.0	39.0	36.0	39.0
25	37.32523184097262	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	8.0
18	30.0
19	126.0
20	290.0
21	559.0
22	1006.0
23	1452.0
24	1904.0
25	2405.0
26	2791.0
27	3215.0
28	3864.0
29	4508.0
30	5474.0
31	6681.0
32	8618.0
33	12303.0
34	21331.0
35	63853.0
36	272114.0
37	38207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.63996982739495	15.799130319030926	10.857478812619249	26.703421040954872
2	18.421262812264278	18.993876736034075	35.649154723343834	26.93570572835781
3	21.243288813950393	21.796601144784134	27.449527443759152	29.510582597506325
4	14.457114966499535	15.039268758042331	34.027599059324665	36.47601721613347
5	15.744109686293651	34.23814172250078	32.802502551359986	17.215246039845585
6	35.07609708479389	32.775879664551624	17.121844078626257	15.02617917202822
7	30.3232462173315	28.533300794249456	21.003239117895017	20.140213870524025
8	27.60904290721924	32.35834405644052	19.717797399831387	20.31481563650885
9	26.06402804277411	16.09220393131295	18.45210098948396	39.391667036428984
10	16.543461862714647	25.48808625815326	31.716954341749126	26.251497537382974
11	36.36597595065892	20.99636153880286	23.177441540577718	19.46022096996051
12	25.67089674757066	22.017571105293516	28.25642277144252	24.055109375693302
13	29.20819097484137	19.779251896880684	24.91480676221325	26.09775036606469
14	24.07041753560811	18.940630962417355	24.536983626924613	32.45196787504992
15	25.339219949416513	26.276123707680703	21.683897590628746	26.70075875227404
16	26.068243333185425	24.994897280028397	23.285042374761503	25.65181701202467
17	25.35497182411146	24.905488751830323	24.37258730088299	25.366952123175224
18	25.17903891378622	25.00199671651063	24.931446066468474	24.887518303234682
19	25.90362514975374	24.430713937081244	24.75462572658295	24.911035186582065
20	25.61365754093269	24.790122908994096	24.370812441762435	25.225407108310776
21	25.52158672405378	24.346408128854772	24.66987620357634	25.46212894351511
22	25.239384123885166	24.165594355948	24.519678750499178	26.07534276966766
23	25.221857390069662	24.008741181168745	24.850246261702978	25.919155167058616
24	25.05723920663797	24.977148688822826	24.561831654612416	25.40378044992679
25	25.76696099747083	24.092603274615076	24.5656032302436	25.574832497670496
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	2.5
3	3.0
4	3.0
5	3.0
6	29.5
7	56.0
8	56.0
9	56.0
10	129.0
11	202.0
12	202.0
13	202.0
14	456.0
15	710.0
16	710.0
17	710.0
18	1218.0
19	1726.0
20	1726.0
21	1726.0
22	2646.0
23	3566.0
24	3566.0
25	3566.0
26	5483.5
27	7401.0
28	7401.0
29	7401.0
30	10409.0
31	13417.0
32	13417.0
33	13417.0
34	17558.5
35	21700.0
36	21700.0
37	21700.0
38	28158.0
39	34616.0
40	34616.0
41	34616.0
42	41160.5
43	47705.0
44	47705.0
45	47705.0
46	53956.5
47	60208.0
48	60208.0
49	60208.0
50	63126.0
51	66044.0
52	66044.0
53	66044.0
54	65602.5
55	65161.0
56	65161.0
57	65161.0
58	59100.5
59	53040.0
60	53040.0
61	53040.0
62	45477.0
63	37914.0
64	37914.0
65	37914.0
66	29978.5
67	22043.0
68	22043.0
69	22043.0
70	16242.0
71	10441.0
72	10441.0
73	10441.0
74	6996.0
75	3551.0
76	3551.0
77	3551.0
78	2257.5
79	964.0
80	964.0
81	964.0
82	589.5
83	215.0
84	215.0
85	215.0
86	131.0
87	47.0
88	47.0
89	47.0
90	27.5
91	8.0
92	8.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	450740.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.89733647848438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.75679196748804	59.77311887977894
2	7.045594401356051	9.708453763223073
3	2.2534522749919903	4.657705788849878
4	1.100535796562326	3.0329594032948557
5	0.6756966124771757	2.327684843360602
6	0.4562438706054751	1.8860392481610908
7	0.32708999873829936	1.5774940791273748
8	0.23063236039020954	1.2711964269305072
9	0.18136364076957565	1.124592460475798
>10	0.9098081884654861	11.060476003453815
>50	0.05007374625087505	2.36677367464077
>100	0.01271714190451489	1.2135054287033946
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCTC	55	5.8867226E-7	17.272728	8
TTAGGAG	35	0.0021669867	16.285713	3
AGGGACG	55	1.1247666E-5	15.545455	5
ACGCTCT	65	3.3593042E-6	14.615386	9
ATAGCAC	55	1.9552972E-4	13.818182	3
GTATAAT	50	0.0014966683	13.299999	1
CTAGCAC	50	0.0014966683	13.299999	3
TAGCACC	65	5.435752E-5	13.153847	4
TAGCACA	65	5.435752E-5	13.153847	4
CGCTCTC	65	5.435752E-5	13.153847	10
TATGTTG	95	7.3410774E-8	13.0	5
GGACGCT	75	1.4730769E-5	12.666666	7
GGGACGC	70	1.08759064E-4	12.214285	6
GTTTAAT	55	0.0030626385	12.090909	1
ACCGTGA	55	0.0030626385	12.090909	12
AGTACCA	55	0.0030626385	12.090909	5
GTAGGGA	80	2.8557957E-5	11.875	3
TAGGGAC	80	2.8557957E-5	11.875	4
TGAGCTA	80	2.8557957E-5	11.875	18
TGCTCTA	75	2.067594E-4	11.4	10
>>END_MODULE