FastQCFastQC Report
Mon 15 Aug 2016
SRR2920807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450740
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCA307.7111076E-419.09
GACTGAG551.9552972E-413.8181827
GTATTGG500.001496668313.2999991
CTCAATA655.435752E-513.1538472
CTTAATA604.0820174E-412.6666672
CGCCTAG701.08759064E-412.21428515
CCGCTCC658.0040435E-411.6923089
TTAAACA658.0040435E-411.6923084
CCGCCTA752.067594E-411.414
TAAACAG855.3032116E-511.1764715
TTAATAC600.00586469611.0833343
GTCAGGC600.00586469611.08333415
CGTACAT600.00586469611.0833345
CCTAGAG700.001487846810.8571422
AGGGTTG700.001487846810.8571425
CCCCGTC700.001487846810.85714211
GCCTAGG700.001487846810.8571421
ACAGTCT803.7594917E-410.68758
TACTTTT909.479816E-510.5555554
GTCCGCC909.479816E-510.55555512
Produced by FastQC (version 0.11.5)