FastQCFastQC Report
Mon 15 Aug 2016
SRR2920806_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920806_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31458916
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA502950.044.895131
GGTATCA270100.042.3705371
TCAACGC644800.034.3885844
CAACGCA658100.033.686635
TATCAAC670150.033.2138942
ATCAACG676100.032.8265723
AACGCAG701650.031.8435386
GTACATG1297300.029.1663571
TACATGG1336150.027.9484312
ACATGGG1377200.026.5192053
ACGCAGA857450.025.9100917
CGCAGAG904050.024.4633088
CATGGGG1089100.023.4799864
TACCTGG284900.022.1517222
GTACCTG313200.021.1592961
GCAGAGT1035800.020.9211799
ATGGGGG604300.020.8548455
GAGTACA914100.019.3312631
AGTACAT888600.018.6381452
AGAGTAC894750.018.57154810-11
Produced by FastQC (version 0.11.5)