##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920806_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31458916
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.508802274051654	31.0	30.0	34.0	25.0	34.0
2	29.602904562890853	31.0	30.0	34.0	25.0	34.0
3	29.738060841002913	31.0	31.0	34.0	25.0	34.0
4	33.15566178440478	37.0	35.0	37.0	27.0	37.0
5	33.12146909321351	37.0	35.0	37.0	27.0	37.0
6	33.19857864778303	37.0	35.0	37.0	27.0	37.0
7	33.09446250468389	37.0	35.0	37.0	26.0	37.0
8	33.07448209595016	37.0	35.0	37.0	26.0	37.0
9	34.49789706676479	39.0	35.0	39.0	27.0	39.0
10-11	34.38256634462548	39.0	35.0	39.0	26.5	39.0
12-13	34.24453971967756	39.0	35.0	39.0	25.0	39.0
14-15	35.20017662083461	39.0	36.0	41.0	25.0	41.0
16-17	35.08827907484161	39.0	36.0	41.0	24.5	41.0
18-19	34.971158764656735	39.0	35.0	41.0	24.0	41.0
20-21	34.86578274025717	39.0	35.0	41.0	22.5	41.0
22-23	34.64218034403983	39.0	34.5	40.0	20.5	41.0
24-25	34.44933237686893	39.0	34.0	40.0	19.0	41.0
26-27	34.48525605586664	39.0	34.5	40.0	18.5	41.0
28-29	34.335338684269985	39.0	34.0	40.0	17.5	41.0
30-31	34.147792218905444	39.0	34.0	40.0	16.5	41.0
32-33	33.93064284223907	38.0	34.0	40.0	15.5	41.0
34-35	33.56541341093889	38.0	33.0	40.0	11.0	41.0
36-37	33.24710377814671	38.0	33.0	40.0	8.5	41.0
38-39	33.07871895522401	38.0	33.0	40.0	8.0	41.0
40-41	32.86721365415134	38.0	32.5	40.0	6.5	41.0
42-43	32.62624015716244	38.0	32.0	40.0	2.0	41.0
44-45	32.34278406159958	38.0	31.5	40.0	2.0	41.0
46-47	32.04361513918661	37.5	31.0	40.0	2.0	41.0
48-49	31.885733332324612	37.0	31.0	40.0	2.0	41.0
50-51	31.182362656742526	36.0	30.0	39.0	2.0	40.5
52-53	31.52018569234871	37.0	31.0	39.0	2.0	40.0
54-55	31.939370336218833	37.0	31.0	40.0	2.0	41.0
56-57	31.814522042018233	37.0	31.0	40.0	2.0	41.0
58-59	31.54411986096406	36.5	31.0	40.0	2.0	41.0
60-61	31.17373411722133	36.0	31.0	39.5	2.0	41.0
62-63	30.766469114829007	35.5	30.0	39.0	2.0	41.0
64-65	30.29260377248854	35.0	29.5	39.0	2.0	40.0
66-67	29.90236763084907	35.0	29.0	38.0	2.0	40.0
68-69	29.465046745412334	35.0	29.0	37.5	2.0	40.0
70-71	29.021985102728905	34.5	28.0	37.0	2.0	39.0
72-73	28.554546698303273	34.0	27.5	36.0	2.0	39.0
74-75	28.118719697779795	34.0	26.5	36.0	2.0	38.5
76-77	27.62505192486607	34.0	26.0	35.0	2.0	37.5
78-79	27.19988657587566	34.0	26.0	35.0	2.0	37.0
80-81	26.775830975867066	33.0	25.0	35.0	2.0	36.5
82-83	26.248244186163312	33.0	23.5	35.0	2.0	36.0
84-85	25.86743540368651	33.0	22.0	35.0	2.0	35.5
86-87	25.472589964638324	32.5	20.0	35.0	2.0	35.0
88-89	25.0055876527977	32.0	18.0	35.0	2.0	35.0
90-91	24.479266418461464	32.0	10.0	34.0	2.0	35.0
92-93	23.912846122860685	31.5	2.0	34.0	2.0	35.0
94-95	23.24851876650804	31.0	2.0	34.0	2.0	35.0
96-97	22.717571117199334	31.0	2.0	34.0	2.0	35.0
98	22.33174048336567	31.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1601721.0
3	227809.0
4	164956.0
5	134309.0
6	124133.0
7	122248.0
8	135502.0
9	154031.0
10	163782.0
11	172679.0
12	184440.0
13	202167.0
14	175892.0
15	176548.0
16	194348.0
17	215014.0
18	227498.0
19	221038.0
20	228719.0
21	243508.0
22	263851.0
23	289423.0
24	324023.0
25	366525.0
26	416579.0
27	479651.0
28	557490.0
29	654429.0
30	779075.0
31	947348.0
32	1185156.0
33	1534049.0
34	2111928.0
35	2940181.0
36	4311339.0
37	5479935.0
38	3413433.0
39	334115.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.00596758809395	16.713009546479913	12.372053978530483	24.908968886895646
2	16.683871779271918	18.548380070762583	38.716145657955494	26.051602492010005
3	20.19435386070639	23.12196550661004	27.450259947811684	29.23342068487189
4	13.527846929787493	14.565063482594201	34.95945537490289	36.94763421271542
5	15.259983886049222	36.2389544107727	32.78252850387965	15.718533199298435
6	35.22111458558136	32.98515754466681	17.15014353226071	14.643584337491125
7	31.603191281538763	29.8580253758648	19.606460043041384	18.93232329955505
8	28.772104827363105	33.24039651657174	18.683832071184327	19.303666584880826
9	28.041868956959608	15.229908112536364	18.065940352172337	38.662282578331684
10-11	27.012403749658944	24.344271319342567	26.486668826414846	22.156656104583647
12-13	27.43293672875895	21.392067306668384	26.869677535184504	24.30531842938816
14-15	24.45946403652626	22.971904869008185	23.942383371074634	28.626247723390925
16-17	25.03240845875039	25.89500109189846	24.524695939379107	24.54789450997204
18-19	25.01834122786003	25.34921721698831	25.78604495542885	23.84639659972281
20-21	25.68936653442974	24.509641069827058	25.552912994666354	24.248079401076847
22-23	25.318554363495753	24.282782456335006	25.47450108575326	24.92416209441598
24-25	24.97339317211271	24.514015108513977	25.53657242085955	24.97601929851376
26-27	24.770873293840985	24.687804379028723	25.551508891218088	24.9898134359122
28-29	24.894598431020153	24.944274575449004	25.303170961546872	24.857956031983967
30-31	25.271938340734874	24.713151409523455	25.304140791174074	24.7107694585676
32-33	25.003140182783763	24.738355200559262	25.370427090934033	24.888077525722945
34-35	25.089469938620784	24.83908622477754	25.444300533470503	24.627143303131177
36-37	25.123811852756084	24.760286099895506	25.379369740886553	24.736532306461854
38-39	25.224340063898744	24.792563481685253	25.24810520752711	24.734991246888892
40-41	25.267152718560908	24.58473794240183	25.398734989497495	24.749374349539764
42-43	25.00326985988327	24.68103814402146	25.3634766664236	24.952215329671663
44-45	24.882051441874133	24.75095216295689	25.402104233474354	24.964892161694625
46-47	25.10230774079029	24.552516781598506	25.271113517546546	25.074061960064654
48-49	25.00165152153454	24.712756952450786	25.248804372625717	25.03678715338896
50-51	24.894650648208298	24.883510667963687	25.296165608903276	24.925673074924738
52-53	25.004186414150574	24.790153445314637	25.303856207689968	24.90180393284482
54-55	24.742405761069826	24.63238515289176	25.612277159987862	25.01293192605055
56-57	24.853952103900394	24.714628703184996	25.32330711138081	25.108112081533797
58-59	24.9437628898994	24.719721216424002	25.38816489084027	24.94835100283633
60-61	25.09789329931732	24.6710991902144	25.47710564892494	24.75390186154334
62-63	24.972588795920593	24.686855451935063	25.509440636454663	24.83111511568968
64-65	24.860784646478493	24.571094898567786	25.503128857195616	25.06499159775811
66-67	24.87774271796428	24.78599903887422	25.32203975400752	25.014218489153983
68-69	24.82047095219256	24.58825662581334	25.539904175611444	25.051368246382655
70-71	24.831383487806175	24.460640369312582	25.549399253604864	25.15857688927638
72-73	24.725566279148264	24.78762791806536	25.310924471111484	25.175881331674887
74-75	24.66833528429756	24.782768772562562	25.482917488715152	25.06597845442473
76-77	24.694713771264894	24.425010763357893	25.515693441697447	25.364582023679773
78-79	24.633592802299937	24.44613355205653	25.59282859164693	25.3274450539966
80-81	24.79241254810455	24.628191726126445	25.353325653527637	25.22607007224137
82-83	24.856618610178234	24.600210857660038	25.45491453537645	25.08825599678528
84-85	24.770752522349184	24.65609448581546	25.367910719523806	25.20524227231155
86-87	24.58692027422014	24.770081923221884	25.395345244217022	25.247652558340956
88-89	24.614060391406497	24.43878036185145	25.51953078953137	25.427628457210684
90-91	24.754523125593387	24.399857179527977	25.5521533485598	25.293466346318837
92-93	24.74948058114921	24.486191680114764	25.559932260717037	25.204395478018988
94-95	24.771299881468767	24.318027367618637	25.52151636754741	25.38915638336519
96-97	24.617456627690242	24.290526514206306	25.597667692452735	25.494349165650714
98	24.644703269053473	24.24744916599293	25.481422515571246	25.626425049382355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	462.0
1	459.0
2	1043.5
3	1776.0
4	1775.5
5	2019.0
6	3271.0
7	5711.5
8	8661.0
9	9875.0
10	9492.0
11	9760.5
12	10962.5
13	12373.0
14	14421.5
15	16675.0
16	18384.5
17	19600.5
18	20139.5
19	20268.5
20	20015.0
21	19795.0
22	20497.5
23	22294.0
24	25364.0
25	30615.0
26	38626.5
27	49078.0
28	63839.0
29	85639.0
30	114620.0
31	154332.0
32	205578.5
33	259648.5
34	322036.0
35	395426.5
36	465116.5
37	524465.0
38	583560.5
39	638502.5
40	690262.0
41	753137.0
42	829124.5
43	921230.5
44	1010797.0
45	1091527.0
46	1180163.0
47	1265394.5
48	1335610.5
49	1388738.5
50	1409497.0
51	1424520.5
52	1454723.0
53	1482218.5
54	1484709.5
55	1456961.5
56	1391584.5
57	1304823.0
58	1207547.5
59	1077680.5
60	934804.0
61	794330.5
62	660771.5
63	525621.0
64	397388.0
65	298525.5
66	226429.0
67	172258.0
68	131058.0
69	99902.5
70	73161.0
71	52433.0
72	37963.5
73	26601.5
74	18659.5
75	12523.0
76	7654.5
77	4412.5
78	2499.5
79	1427.5
80	872.5
81	584.0
82	441.5
83	337.0
84	262.5
85	210.5
86	165.0
87	150.0
88	126.5
89	103.5
90	95.0
91	88.0
92	79.5
93	65.0
94	62.0
95	51.0
96	39.0
97	31.0
98	19.5
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48678091768959875
2	0.02848794917154806
3	0.0418990915008006
4	0.02822093424960987
5	0.03302720284449725
6	0.01703809501891292
7	0.04249033882794945
8	0.008830564918384345
9	0.0
10-11	4.768123606039063E-6
12-13	1.3350746096909378E-4
14-15	0.0021345300009701544
16-17	0.007109272296604244
18-19	0.05331874753726416
20-21	0.0880672430035415
22-23	0.09916266663479441
24-25	0.07878688509165414
26-27	0.08449273967354756
28-29	0.06767874646411846
30-31	0.044833076893049974
32-33	0.03719772162524608
34-35	0.3891202099907066
36-37	0.5712259761270859
38-39	0.5250832546169105
40-41	0.5190468101316651
42-43	0.4775593666355191
44-45	0.4519910984854023
46-47	0.442462798146001
48-49	0.4187795281948049
50-51	0.42850491097658927
52-53	0.48409328535032803
54-55	0.5006768192521319
56-57	0.46459801729976963
58-59	0.4413693084656827
60-61	0.452315330890613
62-63	0.44957048106806985
64-65	0.4721157588519579
66-67	0.5026301605560726
68-69	0.44024721004372813
70-71	0.40706424849476697
72-73	0.38628953394325477
74-75	0.37510192658895175
76-77	0.38498465744973537
78-79	0.37584893262056457
80-81	0.38149915909372084
82-83	0.39125632936621213
84-85	0.3678257699661362
86-87	0.37776253956112155
88-89	0.40114700709967244
90-91	0.3823129188558182
92-93	0.3831250892433802
94-95	0.38789639159848993
96-97	0.4026982366461705
98	0.3775527421224559
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3.1458916E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.15259520581597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.66031177441158	27.970423005888843
2	14.723094892853261	11.82340938819107
3	5.837639846588027	7.0318916915217216
4	2.8202200918460303	4.529566229568113
5	1.5976655093404335	3.2075208235420116
6	0.9860015719849892	2.375431319532689
7	0.7199931230995783	2.0236714695952016
8	0.5168944840493475	1.6603723985722039
9	0.3879640612111582	1.401998751379444
>10	2.389008806614713	17.911474051843896
>50	0.21614216486302051	6.042396382947092
>100	0.1342244410906819	10.016256050147412
>500	0.00828553610347379	2.2512204206752093
>1k	0.0025138899412256595	1.5472136963324838
>5k	7.961200492454125E-6	0.023220310203125975
>10k+	3.184480196941633E-5	0.18393401005934462
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	6.357498141385418E-6	0.0	3.178749070692709E-6	2.8608741636234383E-5
2	0.0	9.536247212078128E-6	0.0	1.2714996282770836E-5	2.8608741636234383E-5
3	0.0	9.536247212078128E-6	0.0	1.5893745353463545E-5	3.178749070692709E-5
4	0.0	9.536247212078128E-6	0.0	4.4502486989697925E-5	3.49662397776198E-5
5	0.0	1.9072494424156256E-5	0.0	5.4038734201776056E-5	3.814498884831251E-5
6	6.357498141385418E-6	1.9072494424156256E-5	0.0	5.4038734201776056E-5	6.357498141385418E-5
7	6.357498141385418E-6	2.2251243494848963E-5	0.0	5.721748327246877E-5	6.675373048454689E-5
8	6.357498141385418E-6	2.2251243494848963E-5	0.0	6.039623234316148E-5	9.218372305008856E-5
9	6.357498141385418E-6	2.2251243494848963E-5	0.0	8.582622490870315E-5	9.218372305008856E-5
10-11	6.357498141385418E-6	2.8608741636234383E-5	0.0	1.048987193328594E-4	9.218372305008856E-5
12-13	1.5893745353463545E-5	2.8608741636234383E-5	0.0	1.398649591104792E-4	1.4463308271651828E-4
14-15	1.5893745353463545E-5	3.6555614312966156E-5	0.0	1.6211620260532818E-4	1.7800994795879173E-4
16-17	1.5893745353463545E-5	4.132373791900522E-5	0.0	2.3045930762522144E-4	2.1297618773641152E-4
18-19	1.74831198888099E-5	5.24493596664297E-5	0.0	2.8926616543303656E-4	2.3681680576660686E-4
20-21	1.9072494424156256E-5	5.562810873712241E-5	0.0	3.5443052138223706E-4	2.9085553996838285E-4
22-23	1.9072494424156256E-5	6.039623234316148E-5	0.0	4.3707799722024753E-4	3.353580269580808E-4
24-25	1.9072494424156256E-5	6.198560687850783E-5	0.0	5.46744840159146E-4	3.878073866245105E-4
26-27	1.9072494424156256E-5	6.357498141385418E-5	0.0	6.627691812394298E-4	3.94164884765896E-4
28-29	1.9072494424156256E-5	6.357498141385418E-5	0.0	0.0012476590102468883	4.132373791900522E-4
30-31	1.9072494424156256E-5	6.834310501989325E-5	0.0	0.0036031120716301856	4.2277362640213035E-4
32-33	1.9072494424156256E-5	7.311122862593231E-5	0.0	0.008255211336588965	4.656867388564819E-4
34-35	2.7019367100888028E-5	7.311122862593231E-5	1.5893745353463546E-6	0.013740142858069236	5.038317277047944E-4
36-37	2.8608741636234383E-5	7.787935223197138E-5	4.768123606039064E-6	0.02395028487313422	5.117786003815261E-4
38-39	2.8608741636234383E-5	8.582622490870315E-5	6.357498141385418E-6	0.04327707922294589	5.403873420177605E-4
40-41	2.8608741636234383E-5	9.218372305008856E-5	6.357498141385418E-6	0.06854018746227619	5.531023383005314E-4
42-43	2.8608741636234383E-5	1.0171997026216669E-4	6.357498141385418E-6	0.09390978379547471	5.642279600479559E-4
44-45	2.8608741636234383E-5	1.0171997026216669E-4	6.357498141385418E-6	0.122995337792313	6.150879451790393E-4
46-47	3.178749070692709E-5	1.0807746840355211E-4	6.357498141385418E-6	0.15847017742124364	6.611798067040835E-4
48-49	3.49662397776198E-5	1.0807746840355211E-4	6.357498141385418E-6	0.19981139846013768	7.056822936937814E-4
50-51	3.49662397776198E-5	1.0807746840355211E-4	6.357498141385418E-6	0.24889764160977446	7.21576039047245E-4
52-53	3.49662397776198E-5	1.1284559200959118E-4	6.357498141385418E-6	0.30152342184962766	7.803828968550601E-4
54-55	3.6555614312966156E-5	1.3350746096909378E-4	6.357498141385418E-6	0.35867574076614717	8.248853838447581E-4
56-57	3.814498884831251E-5	1.398649591104792E-4	6.357498141385418E-6	0.4242739959634973	8.503153764102997E-4
58-59	3.814498884831251E-5	1.430437081811719E-4	6.357498141385418E-6	0.5029400885904651	8.709772453698024E-4
60-61	3.814498884831251E-5	1.4622245725186462E-4	6.357498141385418E-6	0.5931418615949768	8.884603652586122E-4
62-63	3.814498884831251E-5	1.4781183178721097E-4	6.357498141385418E-6	0.6926192243877698	9.313734777129638E-4
64-65	3.814498884831251E-5	1.5734807899928912E-4	6.357498141385418E-6	0.7919932778357652	9.345522267836565E-4
66-67	3.9734363383658866E-5	1.7006307528205997E-4	6.357498141385418E-6	0.9062852006725216	9.695184665612763E-4
68-69	4.132373791900522E-5	1.7324182435275265E-4	6.357498141385418E-6	1.0397958403906862	9.99716582732857E-4
70-71	4.132373791900522E-5	1.7642057342344536E-4	6.357498141385418E-6	1.1914825672950715	0.0010553446914699795
72-73	4.132373791900522E-5	1.780099479587917E-4	6.357498141385418E-6	1.3482902589523427	0.0011522965381261072
74-75	4.132373791900522E-5	1.780099479587917E-4	6.357498141385418E-6	1.5090014544684247	0.0012047458977925366
76-77	4.132373791900522E-5	1.811886970294844E-4	6.357498141385418E-6	1.6867904793668034	0.001233354639428771
78-79	4.132373791900522E-5	1.811886970294844E-4	6.357498141385418E-6	1.894998543497176	0.0012524271338529274
80-81	5.562810873712241E-5	1.811886970294844E-4	9.536247212078128E-6	2.1230737893193776	0.0012842146245598545
82-83	5.721748327246877E-5	1.859568206355235E-4	1.2714996282770836E-5	2.36643246067347	0.0013605046022564795
84-85	5.721748327246877E-5	1.954930678476016E-4	1.2714996282770836E-5	2.614122177636381	0.0013668621003978649
86	5.721748327246877E-5	2.0026119145364067E-4	1.2714996282770836E-5	2.806031205906777	0.0013700408494685577
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	50295	0.0	44.89513	1
GGTATCA	27010	0.0	42.370537	1
TCAACGC	64480	0.0	34.388584	4
CAACGCA	65810	0.0	33.68663	5
TATCAAC	67015	0.0	33.213894	2
ATCAACG	67610	0.0	32.826572	3
AACGCAG	70165	0.0	31.843538	6
GTACATG	129730	0.0	29.166357	1
TACATGG	133615	0.0	27.948431	2
ACATGGG	137720	0.0	26.519205	3
ACGCAGA	85745	0.0	25.910091	7
CGCAGAG	90405	0.0	24.463308	8
CATGGGG	108910	0.0	23.479986	4
TACCTGG	28490	0.0	22.151722	2
GTACCTG	31320	0.0	21.159296	1
GCAGAGT	103580	0.0	20.921179	9
ATGGGGG	60430	0.0	20.854845	5
GAGTACA	91410	0.0	19.331263	1
AGTACAT	88860	0.0	18.638145	2
AGAGTAC	89475	0.0	18.571548	10-11
>>END_MODULE