FastQCFastQC Report
Mon 15 Aug 2016
SRR2920806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31458916
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA519300.046.0272141
GGTATCA282500.044.655011
TCAACGC675600.034.621584
CAACGCA689550.033.86445
TATCAAC700950.033.6993142
ATCAACG709750.032.9423
AACGCAG741150.031.661966
GTACATG1320450.027.709021
TACATGG1364550.026.4428922
ACGCAGA911900.025.6134157
ACATGGG1396700.025.1149123
CGCAGAG952800.024.4442678
CATGGGG1092900.022.0319424
TACCTGG283950.021.3991132
GCAGAGT1102350.020.746099
GTACCTG316700.020.0366361
GAGTACA964400.019.3594571
ATGGGGG592850.019.1576355
AGAGTAC964300.018.86734410-11
AGTACAT929550.018.8239292