FastQCFastQC Report
Mon 15 Aug 2016
SRR2920805_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920805_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3535066
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112100.3171086480422148No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC107860.30511452968629155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99800.28231438960404137No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA94370.26695399746426235No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA89140.25215936562429103No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT81100.22941580157202157No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT74910.21190552029297333No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC72680.2055972929501175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66400.18783241953615576No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61140.17295292365121331No Hit
CCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTT60530.17122735473679981No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA55570.15719649930156893No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC54490.15414139368260735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.15151060828850155No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA52600.1487949588494246No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGC49160.13906388169273218No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG48380.13685741652348218No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC45060.12746579554667437No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT42140.11920569516948198No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC41940.11863993486967428No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG40070.1133500760664723No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC39490.11170937119702998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7650.071.943272
TACCTGG64350.071.557552
ATAACGC8950.062.5182953
ACCTGGG74050.061.5027473
GTACCTG77200.061.0909271
TAACGCA9550.058.1060834
CCTAGAG34250.054.634872
CTAGAGG34150.054.2576523
CCCTAGA34950.054.0294721
GTATAAC10850.053.485351
CCTGGGG65550.053.171694
TAGAGGT39350.047.31744
TATCTAA10250.046.982462
TATCACG4350.046.3910032
CTGGGGG48600.045.0113145
ATAAGTA12250.043.0527955
ATATCTA11700.042.5141
TACCGGG5800.040.3285372
CTATAGC9900.040.3045773
GTACCCG12650.038.957161
Produced by FastQC (version 0.11.5)