##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920805_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3535066
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.514190965600076	31.0	30.0	34.0	25.0	34.0
2	29.546852024827825	31.0	30.0	34.0	25.0	34.0
3	29.66699546769424	31.0	30.0	34.0	25.0	34.0
4	33.10932525729364	37.0	35.0	37.0	27.0	37.0
5	33.10627128319528	37.0	35.0	37.0	26.0	37.0
6	33.19891651244984	37.0	35.0	37.0	27.0	37.0
7	33.122426568556286	37.0	35.0	37.0	26.0	37.0
8	33.124517618624374	37.0	35.0	37.0	26.0	37.0
9	34.48449703626467	39.0	35.0	39.0	27.0	39.0
10-11	34.324937921951104	39.0	35.0	39.0	26.0	39.0
12-13	34.14861547139431	38.0	35.0	39.0	25.0	39.0
14-15	35.08506701147871	39.0	35.0	41.0	24.5	41.0
16-17	34.96898869780649	39.0	35.0	41.0	23.5	41.0
18-19	34.85913289879171	39.0	35.0	41.0	23.0	41.0
20-21	34.7619021823072	39.0	35.0	41.0	22.0	41.0
22-23	34.51856853591984	39.0	34.0	40.5	20.0	41.0
24-25	34.32458121008207	39.0	34.0	40.0	18.0	41.0
26-27	34.354571597814584	39.0	34.0	40.0	18.0	41.0
28-29	34.15448367866399	39.0	34.0	40.0	17.0	41.0
30-31	33.89000431675109	38.0	34.0	40.0	15.5	41.0
32-33	33.57964264316423	38.0	33.5	40.0	11.5	41.0
34-35	33.13050463555702	38.0	33.0	40.0	8.0	41.0
36-37	32.807974730881966	38.0	32.5	40.0	4.0	41.0
38-39	32.6309965641377	38.0	32.0	40.0	2.0	41.0
40-41	32.42124362034542	38.0	32.0	40.0	2.0	41.0
42-43	32.200473060474685	38.0	31.5	40.0	2.0	41.0
44-45	31.93830270778537	37.5	31.0	40.0	2.0	41.0
46-47	31.593651716827917	37.0	30.5	40.0	2.0	41.0
48-49	31.399160015682874	37.0	30.0	40.0	2.0	41.0
50-51	30.649232291561177	36.0	29.0	39.0	2.0	40.0
52-53	30.953643298314656	36.5	30.5	39.0	2.0	40.0
54-55	31.326812851584666	37.0	31.0	40.0	2.0	41.0
56-57	31.189821208430054	37.0	31.0	40.0	2.0	41.0
58-59	30.922588574018135	36.0	30.5	40.0	2.0	41.0
60-61	30.468940466740932	35.5	29.5	39.0	2.0	41.0
62-63	29.974869351802766	35.0	29.0	39.0	2.0	40.0
64-65	29.50976219397318	35.0	28.0	38.5	2.0	40.0
66-67	29.117425247505988	35.0	28.0	37.5	2.0	40.0
68-69	28.69235609745334	34.5	27.5	37.0	2.0	39.0
70-71	28.254128635787847	34.0	26.5	36.5	2.0	39.0
72-73	27.79428587754797	34.0	26.0	36.0	2.0	38.5
74-75	27.390811090938612	34.0	26.0	35.5	2.0	37.5
76-77	26.915809775545917	34.0	25.0	35.0	2.0	37.0
78-79	26.517005481651545	33.5	24.0	35.0	2.0	36.0
80-81	26.146102364142564	33.0	22.5	35.0	2.0	36.0
82-83	25.62480078165443	33.0	20.0	35.0	2.0	35.5
84-85	25.256947112161413	33.0	18.5	35.0	2.0	35.0
86-87	24.880811136199437	32.0	15.0	35.0	2.0	35.0
88-89	24.466102754517173	32.0	7.0	35.0	2.0	35.0
90-91	23.972548885933108	32.0	2.0	34.0	2.0	35.0
92-93	23.422786731563143	31.0	2.0	34.0	2.0	35.0
94-95	22.779852483659425	31.0	2.0	34.0	2.0	35.0
96-97	22.232585615091768	31.0	2.0	34.0	2.0	35.0
98	21.85851409846379	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181401.0
3	25087.0
4	19213.0
5	15563.0
6	14532.0
7	14902.0
8	16378.0
9	18177.0
10	19309.0
11	21467.0
12	25643.0
13	31230.0
14	26411.0
15	24074.0
16	26792.0
17	30504.0
18	32454.0
19	28484.0
20	28079.0
21	29674.0
22	32293.0
23	34287.0
24	37516.0
25	42663.0
26	48118.0
27	54646.0
28	62998.0
29	73050.0
30	87298.0
31	106024.0
32	131368.0
33	172796.0
34	252758.0
35	316815.0
36	463370.0
37	629101.0
38	336333.0
39	24253.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.36315185246048	14.352737679404957	11.349174172850821	24.93493629528374
2	19.082913406860083	17.524374977362452	37.61027314462675	25.78243847115071
3	20.309397562538347	27.965350053997266	27.835564163925298	23.88968821953909
4	12.411819934679546	14.710175151855056	35.9466796756728	36.93132523779259
5	12.468249332726618	34.26038490185025	28.29875849797271	24.972607267450417
6	28.517053465001418	31.03965745097601	23.985929324648893	16.457359759373684
7	28.66214584311491	32.33025615075676	21.01169770876829	17.99590029736004
8	33.56392064823967	28.138885383996907	20.130295223809828	18.166898743953592
9	33.29256087439386	17.949820455968858	19.21205997285482	29.545558696782464
10-11	29.216724666526734	22.548843501083148	26.944475718416573	21.289956113973542
12-13	27.484093257323256	21.73851486149801	27.07052148373824	23.706870397440493
14-15	25.501016764844785	22.38298505524703	25.686434728958652	26.429563450949527
16-17	25.33467315757193	22.57572722808018	26.343162483110742	25.746437131237144
18-19	24.67190431005264	23.88160180465897	27.024513331507727	24.42198055378066
20-21	25.820266671876485	23.591568986125605	25.880590130765007	24.707574211232906
22-23	25.50548817758353	22.725740354964874	27.736198779876386	24.03257268757521
24-25	23.259025046665048	24.32898736639273	27.092934007571163	25.319053579371065
26-27	23.554769580677025	23.436660999776468	26.096687651006768	26.911881768539743
28-29	24.727460386568886	24.902463231568227	25.734561844623972	24.63551453723892
30-31	26.824419718456948	24.741580191826483	26.476619423014903	21.957380666701667
32-33	26.21771517415822	24.563974795295255	25.76526208489523	23.453047945651296
34-35	25.784331601955866	23.5432117279238	26.101282173628533	24.571174496491803
36-37	25.427225234879618	24.071059888505275	27.387801711148928	23.113913165466172
38-39	26.954883796337114	25.0709509330709	24.884678068496306	23.089487202095675
40-41	26.159304010885	23.277234109359643	26.472697063052898	24.090764816702464
42-43	24.005879607070995	24.147411488914827	27.322731922098757	24.523976981915418
44-45	24.092906042954134	24.489959483240796	25.99475805901532	25.422376414789756
46-47	24.145781766409293	22.96110421183953	26.27254617223045	26.620567849520732
48-49	23.81707767486789	23.255298596511164	26.93273765554861	25.994886073072333
50-51	23.84078703776711	25.00576443677084	26.423297331708635	24.73015119375341
52-53	25.64402833608114	23.38276823509042	25.351819962341	25.62138346648744
54-55	23.60416711393394	23.636654712841615	27.798564943847637	24.960613229376808
56-57	24.380622900317245	23.502149319048314	26.173171757069387	25.944056023565054
58-59	25.5528093192373	24.932810356476313	25.724081634972855	23.790298689313524
60-61	26.65385925479507	25.165654858392077	25.571533952122977	22.608951934689873
62-63	27.797809208508863	24.291593591495797	25.155109776882355	22.75548742311298
64-65	26.520540032553864	23.06089607835668	25.36975583465184	25.04880805443761
66-67	24.92170220848966	24.756789173492663	26.191044905141236	24.13046371287644
68-69	24.27675023827941	22.885195644871892	26.687009694291923	26.151044422556772
70-71	23.654398016502256	23.309351107848713	26.907835418320197	26.128415457328835
72-73	22.647487890784813	25.852295762921386	25.25329983125415	26.246916515039654
74-75	24.09171668333376	23.710747074431417	25.683082299857485	26.514453942377337
76-77	23.350636928113023	22.867810968403944	26.864072301344972	26.91747980213806
78-79	22.7748873233957	25.000773848520502	26.655148392908313	25.569190435175493
80-81	25.4012947419391	23.67710686661943	25.580525195496378	25.34107319594509
82-83	25.350995973219177	24.003561687995848	26.530840625113168	24.114601713671814
84-85	24.29961422896124	25.420325168738444	25.007933074524612	25.2721275277757
86-87	24.470863803419295	25.154975539444948	24.76020448805357	25.61395616908219
88-89	23.203288453980946	24.408067347292107	26.525701272724973	25.862942926001974
90-91	24.94550035727228	24.70216896650047	26.464873148460548	23.8874575277667
92-93	25.420498399714063	24.932794238828635	25.342454373852185	24.304252987605114
94-95	24.988891405590437	23.85322312547351	25.362742292680394	25.795143176255653
96-97	23.773053928445936	25.285401860647706	25.098021893615673	25.843522317290684
98	25.52819286291792	23.586863824969136	23.686088571291446	27.1988547408215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1083.0
1	1009.0
2	4340.0
3	6733.5
4	3940.0
5	2197.5
6	2566.5
7	4083.0
8	5975.0
9	6253.0
10	5033.0
11	4103.5
12	4034.0
13	4406.5
14	5032.0
15	5493.0
16	5905.0
17	6305.5
18	6451.5
19	6288.0
20	6223.0
21	6367.5
22	6658.0
23	7017.0
24	7317.5
25	7990.0
26	9099.5
27	10212.5
28	11450.5
29	13526.0
30	16049.0
31	19445.5
32	23617.0
33	27389.0
34	31623.0
35	36375.0
36	39963.0
37	41670.0
38	43158.5
39	44062.0
40	45048.5
41	48066.0
42	53033.5
43	61627.0
44	73086.5
45	86896.0
46	103917.5
47	124013.5
48	145514.0
49	167388.0
50	178680.0
51	181533.5
52	182796.5
53	184826.0
54	189377.0
55	190254.5
56	192312.0
57	198173.0
58	194418.5
59	177047.5
60	133575.0
61	85793.0
62	63249.5
63	45168.0
64	30211.5
65	21728.0
66	15984.5
67	11394.0
68	7996.5
69	5892.0
70	4236.0
71	2931.0
72	2122.0
73	1535.5
74	1129.0
75	820.0
76	589.5
77	424.5
78	308.5
79	207.5
80	133.0
81	86.5
82	65.0
83	54.5
84	46.0
85	35.5
86	23.5
87	17.0
88	15.0
89	11.5
90	12.0
91	10.0
92	5.5
93	3.5
94	4.0
95	6.5
96	5.0
97	2.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5019425379893898
2	0.03151284869928878
3	0.043959575295058145
4	0.031229968549384934
5	0.03626523521767345
6	0.017623433339009793
7	0.04636405656924086
8	0.009165316856884709
9	0.0
10-11	0.0
12-13	2.404481274182717E-4
14-15	0.0022488971917355998
16-17	0.007750916107365464
18-19	0.05625071780838038
20-21	0.0925725290560346
22-23	0.1047363755019001
24-25	0.08426999665635664
26-27	0.0890082391672461
28-29	0.07219101425546227
30-31	0.048556377730995685
32-33	0.04073474158615426
34-35	0.40084117241375405
36-37	0.5889281840848233
38-39	0.5447140166548516
40-41	0.5384340773269862
42-43	0.49458765409188965
44-45	0.4673745836711394
46-47	0.45758693048446625
48-49	0.43184483684321595
50-51	0.4424669864721054
52-53	0.5010656095246878
54-55	0.518788050916164
56-57	0.4801890544617837
58-59	0.4556774894726152
60-61	0.4676150317985577
62-63	0.46474379827703355
64-65	0.48666700989458184
66-67	0.5194811072834283
68-69	0.45509758516531235
70-71	0.4192283821575043
72-73	0.39859227522201846
74-75	0.3863152767161914
76-77	0.3967394102401483
78-79	0.3876872454432251
80-81	0.3932316963813406
82-83	0.40348610181535505
84-85	0.37955444113348946
86-87	0.38993614263496074
88-89	0.4137829392718552
90-91	0.3943915049959463
92-93	0.39536744151311465
94-95	0.40009154001650893
96-97	0.41523977204386
98	0.38944110237262897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3535066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.23688264428284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76799017016259	32.75971699180496
2	13.38216367226773	11.572060804488498
3	4.354965432369899	5.648853879578574
4	1.8207695653832072	3.148976000830223
5	0.9962642296025809	2.153767978901182
6	0.6388572221779589	1.657331684505656
7	0.44524262232737066	1.3475632106860889
8	0.3379778698953323	1.169048759762334
9	0.26181680832811316	1.0188128354384989
>10	1.7222687209639371	14.159865824888481
>50	0.15272076790096364	4.5761222197122295
>100	0.10049009672184635	8.531502620580001
>500	0.009865413846044745	2.885050759495415
>1k	0.007614245584799122	6.118526813217012
>5k	8.607408052381616E-4	2.623109139930761
>10k+	1.3242166234433256E-4	0.6296904761802161
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11210	0.3171086480422148	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	10786	0.30511452968629155	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9980	0.28231438960404137	No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA	9437	0.26695399746426235	No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA	8914	0.25215936562429103	No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT	8110	0.22941580157202157	No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT	7491	0.21190552029297333	No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC	7268	0.2055972929501175	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6640	0.18783241953615576	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6114	0.17295292365121331	No Hit
CCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTT	6053	0.17122735473679981	No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA	5557	0.15719649930156893	No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5449	0.15414139368260735	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5356	0.15151060828850155	No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA	5260	0.1487949588494246	No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGC	4916	0.13906388169273218	No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG	4838	0.13685741652348218	No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC	4506	0.12746579554667437	No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT	4214	0.11920569516948198	No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC	4194	0.11863993486967428	No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4007	0.1133500760664723	No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC	3949	0.11170937119702998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.8288014990384904E-5	0.0
2	0.0	0.0	0.0	2.8288014990384904E-5	0.0
3	0.0	0.0	0.0	2.8288014990384904E-5	2.8288014990384904E-5
4	0.0	0.0	0.0	2.8288014990384904E-5	2.8288014990384904E-5
5	0.0	0.0	0.0	2.8288014990384904E-5	2.8288014990384904E-5
6	0.0	0.0	0.0	2.8288014990384904E-5	2.8288014990384904E-5
7	0.0	0.0	0.0	2.8288014990384904E-5	2.8288014990384904E-5
8	0.0	0.0	0.0	2.8288014990384904E-5	2.8288014990384904E-5
9	0.0	0.0	0.0	5.657602998076981E-5	2.8288014990384904E-5
10-11	0.0	0.0	0.0	1.5558408244711696E-4	2.8288014990384904E-5
12-13	0.0	0.0	0.0	2.121601124278868E-4	2.8288014990384904E-5
14-15	0.0	1.4144007495192452E-5	0.0	2.545921349134641E-4	2.8288014990384904E-5
16-17	0.0	2.8288014990384904E-5	0.0	3.3945617988461884E-4	2.8288014990384904E-5
18-19	0.0	2.8288014990384904E-5	0.0	4.2432022485577354E-4	8.486404497115471E-5
20-21	0.0	2.8288014990384904E-5	0.0	5.516162923125056E-4	8.486404497115471E-5
22-23	0.0	2.8288014990384904E-5	0.0	8.910724721971244E-4	8.486404497115471E-5
24-25	0.0	2.8288014990384904E-5	0.0	0.0012446726595769357	8.486404497115471E-5
26-27	0.0	2.8288014990384904E-5	0.0	0.0014851207869952076	8.486404497115471E-5
28-29	0.0	2.8288014990384904E-5	0.0	0.0020508810868029056	8.486404497115471E-5
30-31	0.0	2.8288014990384904E-5	0.0	0.0038047380162067697	8.486404497115471E-5
32-33	0.0	2.8288014990384904E-5	0.0	0.009419908991798173	8.486404497115471E-5
34-35	0.0	4.2432022485577354E-5	0.0	0.0176092893315146	1.4144007495192452E-4
36-37	0.0	9.900805246634717E-5	0.0	0.029263951507553183	1.4144007495192452E-4
38-39	0.0	1.4144007495192452E-4	0.0	0.05482217305136594	1.5558408244711696E-4
40-41	0.0	1.4144007495192452E-4	0.0	0.09654699516218368	2.2630411992307923E-4
42-43	0.0	1.4144007495192452E-4	0.0	0.13513184760906868	2.2630411992307923E-4
44-45	0.0	1.4144007495192452E-4	0.0	0.17269833151629985	2.687361424086566E-4
46-47	0.0	1.4144007495192452E-4	0.0	0.21426756954467047	3.111681648942339E-4
48-49	0.0	1.4144007495192452E-4	0.0	0.26947163079840664	3.111681648942339E-4
50-51	0.0	1.4144007495192452E-4	0.0	0.33849438737494575	3.111681648942339E-4
52-53	0.0	1.4144007495192452E-4	0.0	0.4256073295378361	3.536001873798113E-4
54-55	0.0	1.4144007495192452E-4	0.0	0.5307821692720871	3.6774419487500376E-4
56-57	0.0	1.4144007495192452E-4	0.0	0.6306954382181267	3.6774419487500376E-4
58-59	0.0	1.4144007495192452E-4	0.0	0.7581470897573058	3.6774419487500376E-4
60-61	0.0	1.4144007495192452E-4	0.0	0.8983283480421582	3.6774419487500376E-4
62-63	0.0	1.4144007495192452E-4	0.0	1.102553672265242	3.6774419487500376E-4
64-65	0.0	1.4144007495192452E-4	0.0	1.2796083580900612	3.6774419487500376E-4
66-67	0.0	1.4144007495192452E-4	0.0	1.4843711545979623	3.6774419487500376E-4
68-69	0.0	1.4144007495192452E-4	0.0	1.7103923943711377	3.6774419487500376E-4
70-71	0.0	1.4144007495192452E-4	0.0	1.952071050441491	4.2432022485577354E-4
72-73	0.0	1.4144007495192452E-4	0.0	2.1486303226021803	4.2432022485577354E-4
74-75	0.0	1.4144007495192452E-4	0.0	2.38558488016914	4.2432022485577354E-4
76-77	0.0	1.5558408244711696E-4	0.0	2.6868239518017485	4.2432022485577354E-4
78-79	0.0	1.6972808994230942E-4	0.0	3.0279066925483145	4.2432022485577354E-4
80-81	0.0	1.6972808994230942E-4	0.0	3.3407014183044956	4.5260823984615846E-4
82-83	0.0	1.6972808994230942E-4	0.0	3.6811759667287682	4.808962548365434E-4
84-85	0.0	1.6972808994230942E-4	0.0	4.08626034139108	4.808962548365434E-4
86	0.0	1.6972808994230942E-4	0.0	4.438446128021372	4.808962548365434E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	765	0.0	71.94327	2
TACCTGG	6435	0.0	71.55755	2
ATAACGC	895	0.0	62.518295	3
ACCTGGG	7405	0.0	61.502747	3
GTACCTG	7720	0.0	61.090927	1
TAACGCA	955	0.0	58.106083	4
CCTAGAG	3425	0.0	54.63487	2
CTAGAGG	3415	0.0	54.257652	3
CCCTAGA	3495	0.0	54.029472	1
GTATAAC	1085	0.0	53.48535	1
CCTGGGG	6555	0.0	53.17169	4
TAGAGGT	3935	0.0	47.3174	4
TATCTAA	1025	0.0	46.98246	2
TATCACG	435	0.0	46.391003	2
CTGGGGG	4860	0.0	45.011314	5
ATAAGTA	1225	0.0	43.052795	5
ATATCTA	1170	0.0	42.514	1
TACCGGG	580	0.0	40.328537	2
CTATAGC	990	0.0	40.304577	3
GTACCCG	1265	0.0	38.95716	1
>>END_MODULE