FastQCFastQC Report
Mon 15 Aug 2016
SRR2920805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3535066
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96640.2733753768670797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95130.2691038866035316No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC87900.2486516517654833No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT81380.23020786599175233No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA80800.22856716112231004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63780.1804209596086749No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60200.17029385024211713No Hit
CTCTCAGGCGAAGGACCAGGTGCAGGGTTGACTCCTTCTGGATGTTGTAA59790.16913404162751136No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA59280.1676913528630017No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT57670.16313698244954974No Hit
CTTTATCCTGGATCTTGGCCTTCACGTTCTCGATGGTGTCACTGGGCTCC56340.15937467645582853No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA55050.1557255225220689No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC54080.15298158506800155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52020.14715425397998227No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG49930.1412420588469918No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT48790.13801722513808795No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC48310.13665940041854946No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC47130.13332141464968406No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGCCTC46470.13145440566031866No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGCC45700.12927622850605902No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC40650.11499078093591462No Hit
TCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACT36690.10378872699972222No Hit
TCACAAAGATCTGCATGCCACCTCTGAGGCGAAGGACCAGGTGCAGGGTG36520.10330783074488567No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA35770.1011862296206068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG67300.073.3587652
TATAACG7600.072.456632
ATAACGC8250.066.750763
TAACGCA8550.066.075314
ACCTGGG77500.062.113853
GTACCTG80950.062.0461
CCTGGGG64700.058.4072954
GTATAAC11150.053.3719831
TATCTAA10600.053.293572
CTGGGGG43700.052.2387545
TATCACG3950.051.6779252
ATCGAGA30500.048.2518583
TACCCGG9950.047.7101362
TCGAGAA31350.046.944264
CCATCGA31400.046.6981051
CGAGAAC24550.046.3968935
CATCGAG32050.045.9163552
AGAACGT25500.044.85457
CCTAGAG16000.044.8013082
CCCTAGA16250.044.531611
Produced by FastQC (version 0.11.5)